Glucocorticoid induced the expression of mRNA and the secretion of lipocortin 1 in rat astrocytoma cells

Haruo Mizuno, Kenji Uemura, Akihiko Moriyama, Yoshiro Wada, Kiyofumi Asai, Shigeki Kimura, Taiji Kato

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24 Citations (Scopus)

Abstract

The lipocortins area family of structurally related proteins that have been shown to be implicated in multiple aspects of cell biology. Subsequent research has shown that lipocortin 1 (LC1) participates in the physiological and pathological functioning of the CNS and neuroendocrine system. In the present study, the effects of 12-O-tetradecanoylphorbol 13-acetate (TPA), dibutyryl cyclic AMP (Bt2cAMP) or dexamethasone (DEX) on expression of LC1 were investigated by a sandwich enzyme-immunoassay and reverse transcription polymerase chain reaction (RT-PCR) in rat astrocytoma (C6) cells time-dependent experiments revealed that the intracellular protein content and the mRNA of rat LC1 increased significantly 4 h after TPA (10 nM) or DEX (1 μM) addition. TPA and DEX elicited a prominent induction of LC1 at 10-8 M and 10-6 M, respectively. Bt2cAMP (0.5 mM) also appeared to induce, but the induction was not statistically significant. In addition, DEX increased the extracellular secretion of LC1 without cytotoxicity. These results suggest that LC1 synthesis is chemically induced and selectively released from C6 cells by dexamethasone.

Original languageEnglish
Pages (from-to)256-264
Number of pages9
JournalBrain Research
Volume746
Issue number1-2
DOIs
Publication statusPublished - 23-01-1997

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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