TY - JOUR
T1 - Glucocorticoid induced the expression of mRNA and the secretion of lipocortin 1 in rat astrocytoma cells
AU - Mizuno, Haruo
AU - Uemura, Kenji
AU - Moriyama, Akihiko
AU - Wada, Yoshiro
AU - Asai, Kiyofumi
AU - Kimura, Shigeki
AU - Kato, Taiji
N1 - Funding Information:
This work was supported by the following sources: a Grant-in-Aid for Scientific Research on General Research (B) and on Priority Area, the Ministry of Education, Science, Sports and Culture, Japan, a Grant for Nervous and Mental Disorders from the Ministry of Health and Welfare, Japan, and Special Coordination Funds of the Science and Technology Agency of Japanese Government.
PY - 1997/1/23
Y1 - 1997/1/23
N2 - The lipocortins area family of structurally related proteins that have been shown to be implicated in multiple aspects of cell biology. Subsequent research has shown that lipocortin 1 (LC1) participates in the physiological and pathological functioning of the CNS and neuroendocrine system. In the present study, the effects of 12-O-tetradecanoylphorbol 13-acetate (TPA), dibutyryl cyclic AMP (Bt2cAMP) or dexamethasone (DEX) on expression of LC1 were investigated by a sandwich enzyme-immunoassay and reverse transcription polymerase chain reaction (RT-PCR) in rat astrocytoma (C6) cells time-dependent experiments revealed that the intracellular protein content and the mRNA of rat LC1 increased significantly 4 h after TPA (10 nM) or DEX (1 μM) addition. TPA and DEX elicited a prominent induction of LC1 at 10-8 M and 10-6 M, respectively. Bt2cAMP (0.5 mM) also appeared to induce, but the induction was not statistically significant. In addition, DEX increased the extracellular secretion of LC1 without cytotoxicity. These results suggest that LC1 synthesis is chemically induced and selectively released from C6 cells by dexamethasone.
AB - The lipocortins area family of structurally related proteins that have been shown to be implicated in multiple aspects of cell biology. Subsequent research has shown that lipocortin 1 (LC1) participates in the physiological and pathological functioning of the CNS and neuroendocrine system. In the present study, the effects of 12-O-tetradecanoylphorbol 13-acetate (TPA), dibutyryl cyclic AMP (Bt2cAMP) or dexamethasone (DEX) on expression of LC1 were investigated by a sandwich enzyme-immunoassay and reverse transcription polymerase chain reaction (RT-PCR) in rat astrocytoma (C6) cells time-dependent experiments revealed that the intracellular protein content and the mRNA of rat LC1 increased significantly 4 h after TPA (10 nM) or DEX (1 μM) addition. TPA and DEX elicited a prominent induction of LC1 at 10-8 M and 10-6 M, respectively. Bt2cAMP (0.5 mM) also appeared to induce, but the induction was not statistically significant. In addition, DEX increased the extracellular secretion of LC1 without cytotoxicity. These results suggest that LC1 synthesis is chemically induced and selectively released from C6 cells by dexamethasone.
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U2 - 10.1016/S0006-8993(96)01259-0
DO - 10.1016/S0006-8993(96)01259-0
M3 - Article
C2 - 9037504
AN - SCOPUS:0031019877
SN - 0006-8993
VL - 746
SP - 256
EP - 264
JO - Brain Research
JF - Brain Research
IS - 1-2
ER -