Glucose induces FGF21 mRNA expression through ChREBP activation in rat hepatocytes

Katsumi Iizuka; Jun Takeda; Yukio Horikawa

doi:10.1016/j.febslet.2009.07.053

Glucose induces FGF21 mRNA expression through ChREBP activation in rat hepatocytes

Katsumi Iizuka, Jun Takeda, Yukio Horikawa

Research output: Contribution to journal › Article › peer-review

156 Citations (Scopus)

Abstract

Fibroblast growth factor 21 (FGF21) has beneficial effects of improving the plasma glucose and lipid profiles in diabetic rodents. Here, we investigated carbohydrate response element binding protein (ChREBP) involvement in the regulation of FGF21 mRNA expression in liver. Glucose stimulation and adenoviral overexpression of dominant active ChREBP increased FGF21 mRNA. Consistently, adenoviral expression of dominant negative Mlx inhibited glucose induction of FGF21 mRNA. Furthermore, deletion studies of mouse FGF21 gene promoter (-2000 to +65 bp) revealed a glucose responsive region between -74 and -52 bp. These findings suggest that FGF21 expression is regulated by ChREBP.

Original language	English
Pages (from-to)	2882-2886
Number of pages	5
Journal	FEBS Letters
Volume	583
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2009.07.053
Publication status	Published - 03-09-2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2009.07.053

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@article{2b2d119834c14b25acdd684c7b8b0aab,

title = "Glucose induces FGF21 mRNA expression through ChREBP activation in rat hepatocytes",

abstract = "Fibroblast growth factor 21 (FGF21) has beneficial effects of improving the plasma glucose and lipid profiles in diabetic rodents. Here, we investigated carbohydrate response element binding protein (ChREBP) involvement in the regulation of FGF21 mRNA expression in liver. Glucose stimulation and adenoviral overexpression of dominant active ChREBP increased FGF21 mRNA. Consistently, adenoviral expression of dominant negative Mlx inhibited glucose induction of FGF21 mRNA. Furthermore, deletion studies of mouse FGF21 gene promoter (-2000 to +65 bp) revealed a glucose responsive region between -74 and -52 bp. These findings suggest that FGF21 expression is regulated by ChREBP.",

author = "Katsumi Iizuka and Jun Takeda and Yukio Horikawa",

note = "Funding Information: This work was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (K. Iizuka), a New Energy and Industrial Technology Development Organization grant (Y. Horikawa), a Health and Labor Science Research Grant for Research on Human Genome (J. Takeda) and Tissue Engineering from the Japanese Ministry of Health, Labor and Welfare , the ONO Medical Research Foundation (K. Iizuka), and the Kao Research Council for the Study of Healthcare Science (K. Iizuka). ",

year = "2009",

month = sep,

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doi = "10.1016/j.febslet.2009.07.053",

language = "English",

volume = "583",

pages = "2882--2886",

journal = "FEBS Letters",

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T1 - Glucose induces FGF21 mRNA expression through ChREBP activation in rat hepatocytes

AU - Iizuka, Katsumi

AU - Takeda, Jun

AU - Horikawa, Yukio

N1 - Funding Information: This work was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (K. Iizuka), a New Energy and Industrial Technology Development Organization grant (Y. Horikawa), a Health and Labor Science Research Grant for Research on Human Genome (J. Takeda) and Tissue Engineering from the Japanese Ministry of Health, Labor and Welfare , the ONO Medical Research Foundation (K. Iizuka), and the Kao Research Council for the Study of Healthcare Science (K. Iizuka).

PY - 2009/9/3

Y1 - 2009/9/3

N2 - Fibroblast growth factor 21 (FGF21) has beneficial effects of improving the plasma glucose and lipid profiles in diabetic rodents. Here, we investigated carbohydrate response element binding protein (ChREBP) involvement in the regulation of FGF21 mRNA expression in liver. Glucose stimulation and adenoviral overexpression of dominant active ChREBP increased FGF21 mRNA. Consistently, adenoviral expression of dominant negative Mlx inhibited glucose induction of FGF21 mRNA. Furthermore, deletion studies of mouse FGF21 gene promoter (-2000 to +65 bp) revealed a glucose responsive region between -74 and -52 bp. These findings suggest that FGF21 expression is regulated by ChREBP.

AB - Fibroblast growth factor 21 (FGF21) has beneficial effects of improving the plasma glucose and lipid profiles in diabetic rodents. Here, we investigated carbohydrate response element binding protein (ChREBP) involvement in the regulation of FGF21 mRNA expression in liver. Glucose stimulation and adenoviral overexpression of dominant active ChREBP increased FGF21 mRNA. Consistently, adenoviral expression of dominant negative Mlx inhibited glucose induction of FGF21 mRNA. Furthermore, deletion studies of mouse FGF21 gene promoter (-2000 to +65 bp) revealed a glucose responsive region between -74 and -52 bp. These findings suggest that FGF21 expression is regulated by ChREBP.

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DO - 10.1016/j.febslet.2009.07.053

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SN - 0014-5793

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EP - 2886

JO - FEBS Letters

JF - FEBS Letters

IS - 17

ER -

Glucose induces FGF21 mRNA expression through ChREBP activation in rat hepatocytes

Abstract

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