Glycoproteins M and N of pseudorabies virus form a disulfide-linked complex

Alice Jöns, J・m Dijkstra, Thomas C. Mettenleiter

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Genes homologous to the herpes simplex virus UL49.5 open reading frame are conserved throughout the Herpesviridae. In the alphaherpesvirus pseudorabies virus (PrV), the UL49.5 product is an O-glycosylated structural protein of the viral envelope, glycoprotein N (gN) (A. Jons, H. Granzow, R. Kuchling, and T. C. Mettenleiter, J. Virol. 70:1237-1241, 1996). For functional characterization of gN, a gN-negative PrV mutant, PrV-gNβ, and the corresponding rescuant, PrV-gNOR, were constructed, gN-negative PrV was able to productively replicate on noncomplementing cells, and one-step growth in cell culture was only slightly reduced compared to that of wild-type PrV. However, penetration was significantly delayed. In indirect immunofluorescence assays with rabbit serum directed against baculovirus- expressed gN, specific staining of wild-type PrV-infected cells occurred only after permeabilization of cells, whereas live cells failed to react with the antiserum. This indicates the lack of surface accessibility of gN in the plasma membrane of a PrV-infected cell. Western blot analyses and radioimmunoprecipitation experiments under reducing and nonreducing conditions led to the discovery of a heteromeric complex composed of gM and gN. The complex was stable in the absence of 2-mercaptoethanol but dissociated after the addition of the reducing agent, indicating that the partners are linked by disulfide bonds. Finally, gN was absent from gM- negative PrV virions, whereas gM was readily detected in virions in the absence of gN. Thus, gM appears to be required for virion localization of gN.

Original languageEnglish
Pages (from-to)550-557
Number of pages8
JournalJournal of Virology
Volume72
Issue number1
Publication statusPublished - 01-01-1998

Fingerprint

Suid herpesvirus 1
Suid Herpesvirus 1
sulfides
Disulfides
glycoproteins
Glycoproteins
Virion
virion
Radioimmunoprecipitation Assay
Viral Envelope Proteins
Viral Structural Proteins
Mercaptoethanol
Baculoviridae
Herpesviridae
cells
Reducing Agents
Membrane Glycoproteins
Simplexvirus
Indirect Fluorescent Antibody Technique
Pseudorabies virus glycoprotein N

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Jöns, Alice ; Dijkstra, J・m ; Mettenleiter, Thomas C. / Glycoproteins M and N of pseudorabies virus form a disulfide-linked complex. In: Journal of Virology. 1998 ; Vol. 72, No. 1. pp. 550-557.
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Glycoproteins M and N of pseudorabies virus form a disulfide-linked complex. / Jöns, Alice; Dijkstra, J・m; Mettenleiter, Thomas C.

In: Journal of Virology, Vol. 72, No. 1, 01.01.1998, p. 550-557.

Research output: Contribution to journalArticle

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AB - Genes homologous to the herpes simplex virus UL49.5 open reading frame are conserved throughout the Herpesviridae. In the alphaherpesvirus pseudorabies virus (PrV), the UL49.5 product is an O-glycosylated structural protein of the viral envelope, glycoprotein N (gN) (A. Jons, H. Granzow, R. Kuchling, and T. C. Mettenleiter, J. Virol. 70:1237-1241, 1996). For functional characterization of gN, a gN-negative PrV mutant, PrV-gNβ, and the corresponding rescuant, PrV-gNOR, were constructed, gN-negative PrV was able to productively replicate on noncomplementing cells, and one-step growth in cell culture was only slightly reduced compared to that of wild-type PrV. However, penetration was significantly delayed. In indirect immunofluorescence assays with rabbit serum directed against baculovirus- expressed gN, specific staining of wild-type PrV-infected cells occurred only after permeabilization of cells, whereas live cells failed to react with the antiserum. This indicates the lack of surface accessibility of gN in the plasma membrane of a PrV-infected cell. Western blot analyses and radioimmunoprecipitation experiments under reducing and nonreducing conditions led to the discovery of a heteromeric complex composed of gM and gN. The complex was stable in the absence of 2-mercaptoethanol but dissociated after the addition of the reducing agent, indicating that the partners are linked by disulfide bonds. Finally, gN was absent from gM- negative PrV virions, whereas gM was readily detected in virions in the absence of gN. Thus, gM appears to be required for virion localization of gN.

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