Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival

Megumi Shiota; Masayuki Naya; Takehiro Yamamoto; Takako Hishiki; Takeharu Tani; Hiroyuki Takahashi; Akiko Kubo; Daisuke Koike; Mai Itoh; Mitsuyo Ohmura; Yasuaki Kabe; Yuki Sugiura; Nobuyoshi Hiraoka; Takayuki Morikawa; Keiyo Takubo; Kentaro Suina; Hideaki Nagashima; Oltea Sampetrean; Osamu Nagano; Hideyuki Saya; Shogo Yamazoe; Hiroyuki Watanabe; Makoto Suematsu

doi:10.1038/s41467-018-03899-1

Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival

Megumi Shiota
, Masayuki Naya
, Takehiro Yamamoto
, Takako Hishiki
, Takeharu Tani
, Hiroyuki Takahashi
, Akiko Kubo
, Daisuke Koike
, Mai Itoh
, Mitsuyo Ohmura
, Yasuaki Kabe
, Yuki Sugiura
, Nobuyoshi Hiraoka
, Takayuki Morikawa
, Keiyo Takubo
, Kentaro Suina
, Hideaki Nagashima
, Oltea Sampetrean
, Osamu Nagano
, Hideyuki Saya

Shogo Yamazoe, Hiroyuki Watanabe, Makoto Suematsu

Research output: Contribution to journal › Article › peer-review

103 Citations (Scopus)

Abstract

Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanofève (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues. Furthermore, GNF-SERS combined with quantum chemical calculation identified cysteine-derived glutathione and hypotaurine (HT) as tumour-dominant and parenchyma-dominant metabolites, respectively. CD44 knockdown in cancer diminished glutathione, but not HT in tumours. Mechanisms whereby tumours sustained HT under CD44-knockdown conditions include upregulation of PHGDH, PSAT1 and PSPH that drove glycolysis-dependent activation of serine/glycine-cleavage systems to provide one-methyl group for HT synthesis. HT was rapidly converted into taurine in cancer cells, suggesting that HT is a robust anti-oxidant for their survival under glutathione-suppressed conditions.

Original language	English
Article number	1561
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-03899-1
Publication status	Published - 01-12-2018
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General
General Physics and Astronomy

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10.1038/s41467-018-03899-1

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Shiota, M., Naya, M., Yamamoto, T., Hishiki, T., Tani, T., Takahashi, H., Kubo, A., Koike, D., Itoh, M., Ohmura, M., Kabe, Y., Sugiura, Y., Hiraoka, N., Morikawa, T., Takubo, K., Suina, K., Nagashima, H., Sampetrean, O., Nagano, O., ... Suematsu, M. (2018). Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival. Nature communications, 9(1), Article 1561. https://doi.org/10.1038/s41467-018-03899-1

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title = "Gold-nanof{\`e}ve surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival",

abstract = "Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanof{\`e}ve (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues. Furthermore, GNF-SERS combined with quantum chemical calculation identified cysteine-derived glutathione and hypotaurine (HT) as tumour-dominant and parenchyma-dominant metabolites, respectively. CD44 knockdown in cancer diminished glutathione, but not HT in tumours. Mechanisms whereby tumours sustained HT under CD44-knockdown conditions include upregulation of PHGDH, PSAT1 and PSPH that drove glycolysis-dependent activation of serine/glycine-cleavage systems to provide one-methyl group for HT synthesis. HT was rapidly converted into taurine in cancer cells, suggesting that HT is a robust anti-oxidant for their survival under glutathione-suppressed conditions.",

author = "Megumi Shiota and Masayuki Naya and Takehiro Yamamoto and Takako Hishiki and Takeharu Tani and Hiroyuki Takahashi and Akiko Kubo and Daisuke Koike and Mai Itoh and Mitsuyo Ohmura and Yasuaki Kabe and Yuki Sugiura and Nobuyoshi Hiraoka and Takayuki Morikawa and Keiyo Takubo and Kentaro Suina and Hideaki Nagashima and Oltea Sampetrean and Osamu Nagano and Hideyuki Saya and Shogo Yamazoe and Hiroyuki Watanabe and Makoto Suematsu",

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Shiota, M, Naya, M, Yamamoto, T, Hishiki, T, Tani, T, Takahashi, H, Kubo, A, Koike, D, Itoh, M, Ohmura, M, Kabe, Y, Sugiura, Y, Hiraoka, N, Morikawa, T, Takubo, K, Suina, K, Nagashima, H, Sampetrean, O, Nagano, O , Saya, H, Yamazoe, S, Watanabe, H & Suematsu, M 2018, 'Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival', Nature communications, vol. 9, no. 1, 1561. https://doi.org/10.1038/s41467-018-03899-1

TY - JOUR

T1 - Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival

AU - Shiota, Megumi

AU - Naya, Masayuki

AU - Yamamoto, Takehiro

AU - Hishiki, Takako

AU - Tani, Takeharu

AU - Takahashi, Hiroyuki

AU - Kubo, Akiko

AU - Koike, Daisuke

AU - Itoh, Mai

AU - Ohmura, Mitsuyo

AU - Kabe, Yasuaki

AU - Sugiura, Yuki

AU - Hiraoka, Nobuyoshi

AU - Morikawa, Takayuki

AU - Takubo, Keiyo

AU - Suina, Kentaro

AU - Nagashima, Hideaki

AU - Sampetrean, Oltea

AU - Nagano, Osamu

AU - Saya, Hideyuki

AU - Yamazoe, Shogo

AU - Watanabe, Hiroyuki

AU - Suematsu, Makoto

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanofève (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues. Furthermore, GNF-SERS combined with quantum chemical calculation identified cysteine-derived glutathione and hypotaurine (HT) as tumour-dominant and parenchyma-dominant metabolites, respectively. CD44 knockdown in cancer diminished glutathione, but not HT in tumours. Mechanisms whereby tumours sustained HT under CD44-knockdown conditions include upregulation of PHGDH, PSAT1 and PSPH that drove glycolysis-dependent activation of serine/glycine-cleavage systems to provide one-methyl group for HT synthesis. HT was rapidly converted into taurine in cancer cells, suggesting that HT is a robust anti-oxidant for their survival under glutathione-suppressed conditions.

AB - Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanofève (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues. Furthermore, GNF-SERS combined with quantum chemical calculation identified cysteine-derived glutathione and hypotaurine (HT) as tumour-dominant and parenchyma-dominant metabolites, respectively. CD44 knockdown in cancer diminished glutathione, but not HT in tumours. Mechanisms whereby tumours sustained HT under CD44-knockdown conditions include upregulation of PHGDH, PSAT1 and PSPH that drove glycolysis-dependent activation of serine/glycine-cleavage systems to provide one-methyl group for HT synthesis. HT was rapidly converted into taurine in cancer cells, suggesting that HT is a robust anti-oxidant for their survival under glutathione-suppressed conditions.

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DO - 10.1038/s41467-018-03899-1

M3 - Article

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AN - SCOPUS:85045910060

SN - 2041-1723

VL - 9

JO - Nature communications

JF - Nature communications

IS - 1

M1 - 1561

ER -

Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival

Abstract

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