Abstract
The long noncoding RNA Gomafu/MIAT/Rncr2 is thought to function in retinal cell specification, stem cell differentiation and the control of alternative splicing. To further investigate physiological functions of Gomafu, we created mouse knockout (KO) model that completely lacks the Gomafu gene. The KO mice did not exhibit any developmental deficits. However, behavioral tests revealed that the KO mice are hyperactive. This hyperactive behavior was enhanced when the KO mice were treated with the psychostimulant methamphetamine, which was associated with an increase in dopamine release in the nucleus accumbens. RNA sequencing analyses identified a small number of genes affected by the deficiency of Gomafu, a subset of which are known to have important neurobiological functions. These observations suggest that Gomafu modifies mouse behavior thorough a mild modulation of gene expression and/or alternative splicing of target genes.
| Original language | English |
|---|---|
| Article number | 27204 |
| Journal | Scientific reports |
| Volume | 6 |
| DOIs | |
| Publication status | Published - 02-06-2016 |
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Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine. / Ip, Joanna Y.; Sone, Masamitsu; Nashiki, Chieko; Pan, Qun; Kitaichi, Kiyoyuki; Yanaka, Kaori; Abe, Takaya; Takao, Keizo; Miyakawa, Tsuyoshi; Blencowe, Benjamin J.; Nakagawa, Shinichi.
In: Scientific reports, Vol. 6, 27204, 02.06.2016.Research output: Contribution to journal › Article
TY - JOUR
T1 - Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine
AU - Ip, Joanna Y.
AU - Sone, Masamitsu
AU - Nashiki, Chieko
AU - Pan, Qun
AU - Kitaichi, Kiyoyuki
AU - Yanaka, Kaori
AU - Abe, Takaya
AU - Takao, Keizo
AU - Miyakawa, Tsuyoshi
AU - Blencowe, Benjamin J.
AU - Nakagawa, Shinichi
PY - 2016/6/2
Y1 - 2016/6/2
N2 - The long noncoding RNA Gomafu/MIAT/Rncr2 is thought to function in retinal cell specification, stem cell differentiation and the control of alternative splicing. To further investigate physiological functions of Gomafu, we created mouse knockout (KO) model that completely lacks the Gomafu gene. The KO mice did not exhibit any developmental deficits. However, behavioral tests revealed that the KO mice are hyperactive. This hyperactive behavior was enhanced when the KO mice were treated with the psychostimulant methamphetamine, which was associated with an increase in dopamine release in the nucleus accumbens. RNA sequencing analyses identified a small number of genes affected by the deficiency of Gomafu, a subset of which are known to have important neurobiological functions. These observations suggest that Gomafu modifies mouse behavior thorough a mild modulation of gene expression and/or alternative splicing of target genes.
AB - The long noncoding RNA Gomafu/MIAT/Rncr2 is thought to function in retinal cell specification, stem cell differentiation and the control of alternative splicing. To further investigate physiological functions of Gomafu, we created mouse knockout (KO) model that completely lacks the Gomafu gene. The KO mice did not exhibit any developmental deficits. However, behavioral tests revealed that the KO mice are hyperactive. This hyperactive behavior was enhanced when the KO mice were treated with the psychostimulant methamphetamine, which was associated with an increase in dopamine release in the nucleus accumbens. RNA sequencing analyses identified a small number of genes affected by the deficiency of Gomafu, a subset of which are known to have important neurobiological functions. These observations suggest that Gomafu modifies mouse behavior thorough a mild modulation of gene expression and/or alternative splicing of target genes.
UR - http://www.scopus.com/inward/record.url?scp=84973281950&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973281950&partnerID=8YFLogxK
U2 - 10.1038/srep27204
DO - 10.1038/srep27204
M3 - Article
C2 - 27251103
AN - SCOPUS:84973281950
VL - 6
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 27204
ER -