Abstract
GPA1 protein of Saccharomyces cerevisiae is homologous to the α subunit of mammalian G protein. GPA1 transcript was found in haploid cells but was not detected in diploid cells. Disruption of GPA1 resulted in a haploid-specific lethal phenotype, indicating that GPA1 is a haploid-specific essential gene for cell growth. Upon regulation of expression of GPA1 by the galactose-inducible GAL1 promoter, the loss of GPA1 function was found to lead to cell-cycle arrest at the late G1 phase. Mutants that suppress the lethality of the gpa1::HIS3 mutation showed a sterile phenotype that was not cell-type-specific. These results suggest that GPA1 protein may control the signal for mating-factor-mediated cell-cycle arrest.
Original language | English |
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Pages (from-to) | 1011-1019 |
Number of pages | 9 |
Journal | Cell |
Volume | 50 |
Issue number | 7 |
DOIs | |
Publication status | Published - 25-09-1987 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- General Biochemistry,Genetics and Molecular Biology