GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction

Ikuko Miyajima, Masato Nakafuku, Naoki Nakayama, Charles Brenner, Atsushi Miyajima, Kozo Kaibuchi, Ken ichi Arai, Yoshito Kaziro, Kunihiro Matsumoto

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

GPA1 protein of Saccharomyces cerevisiae is homologous to the α subunit of mammalian G protein. GPA1 transcript was found in haploid cells but was not detected in diploid cells. Disruption of GPA1 resulted in a haploid-specific lethal phenotype, indicating that GPA1 is a haploid-specific essential gene for cell growth. Upon regulation of expression of GPA1 by the galactose-inducible GAL1 promoter, the loss of GPA1 function was found to lead to cell-cycle arrest at the late G1 phase. Mutants that suppress the lethality of the gpa1::HIS3 mutation showed a sterile phenotype that was not cell-type-specific. These results suggest that GPA1 protein may control the signal for mating-factor-mediated cell-cycle arrest.

Original languageEnglish
Pages (from-to)1011-1019
Number of pages9
JournalCell
Volume50
Issue number7
DOIs
Publication statusPublished - 25-09-1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction'. Together they form a unique fingerprint.

  • Cite this

    Miyajima, I., Nakafuku, M., Nakayama, N., Brenner, C., Miyajima, A., Kaibuchi, K., Arai, K. I., Kaziro, Y., & Matsumoto, K. (1987). GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction. Cell, 50(7), 1011-1019. https://doi.org/10.1016/0092-8674(87)90167-X