Growth factor midkine is involved in the pathogenesis of diabetic nephropathy

Tomoki Kosugi, Yukio Yuzawa, Waichi Sato, Hanayo Kawai, Seiichi Matsuo, Yoshifumi Takei, Takashi Muramatsu, Kenji Kadomatsu

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)


Diabetic nephropathy is a life-threatening disease associated with diabetes mellitus. Longstanding hyperglycemia induces pathological reactions of glomerular mesangial cells, such as overproduction of extracellular matrix, which finally lead to nephropathy. However, the mechanisms underlying its pathogenesis have not been completely elucidated. Using the Streptozotocin- induced model of diabetes, we report that mice deficient in the growth factor midkine (Mdk-/-) exhibited strikingly milder nephropathy than Mdk +/+ mice, even though both mice showed similar extents of hyperglycemia after Streptozotocin injection. Midkine expression was induced in the glomerular mesangium of Mdk +/+ mice with diabetic nephropathy and in primary cultured mesangial cells exposed to high glucose. Mdk -/- mesangial cells exhibited reduced phosphorylation of protein kinase C and extracellular signal-regulated kinase as well as reduced production of transforming growth factor-β1 on high glucose loading. Addition of exogenous midkine restored extracellular signal-regulated kinase phosphorylation in Mdk -/- cells under high glucose conditions, whereas a midkine antisense oligodeoxynucleotide suppressed midkine in Mdk +/+ cells. Therefore, this study identifies midkine as a key molecule in diabetic nephropathy and suggests that midkine accelerates the intracellular signaling network evoked by hyperglycemia in nephropathy.

Original languageEnglish
Pages (from-to)9-19
Number of pages11
JournalAmerican Journal of Pathology
Issue number1
Publication statusPublished - 01-2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine


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