TY - JOUR
T1 - GTP cyclohydrolase I from Tetrahymena pyriformis
T2 - Cloning of cDNA and expression
AU - Tazawa, Masahiro
AU - Ohtsuki, Masatsugu
AU - Sumi-Ichinose, Chiho
AU - Shiraishi, Hiroaki
AU - Kuroda, Risa
AU - Hagino, Yasumichi
AU - Nakashima, Shigeru
AU - Nozawa, Yoshinori
AU - Ichinose, Hiroshi
AU - Nagatsu, Toshiharu
AU - Nomura, Takahide
N1 - Funding Information:
This study was supported by a grant-in-aid from Fujita Health University.
PY - 2000/9/1
Y1 - 2000/9/1
N2 - A full-length cDNA clone for GTP cyclohydrolase I (EC 3.5.4.16) was isolated from a Tetrahymena pyriformis cDNA library by plaque hybridization. The nucleotide sequence determination revealed that the length of the cDNA insert was 1516 bp. The coding region encoded a protein of 223 amino acid residues with a calculated molecular mass of 25 416 Da. The deduced amino acid sequence of Tetrahymena GTP cyclohydrolase I showed sequence identity with that of Escherichia coli (55%). The identity of T. pyriformis GTP cyclohydrolase I with sequences of Dictyostelium discoideum, Saccharomyces cerevisiae, Drosophila melanogaster, mouse, rat, and human enzymes was less marked and was 30, 30, 25, 28, 28, and 27%, respectively. RNA blot analysis showed a single mRNA species of 2.1 kb in this protozoan. The mRNA level of GTP cyclohydrolase I increased during synchronous cell division induced by intermittent heat treatment. The results suggest that the mRNA expression is associated with the cell cycle of T. pyriformis. (C) 2000 Elsevier Science Inc.
AB - A full-length cDNA clone for GTP cyclohydrolase I (EC 3.5.4.16) was isolated from a Tetrahymena pyriformis cDNA library by plaque hybridization. The nucleotide sequence determination revealed that the length of the cDNA insert was 1516 bp. The coding region encoded a protein of 223 amino acid residues with a calculated molecular mass of 25 416 Da. The deduced amino acid sequence of Tetrahymena GTP cyclohydrolase I showed sequence identity with that of Escherichia coli (55%). The identity of T. pyriformis GTP cyclohydrolase I with sequences of Dictyostelium discoideum, Saccharomyces cerevisiae, Drosophila melanogaster, mouse, rat, and human enzymes was less marked and was 30, 30, 25, 28, 28, and 27%, respectively. RNA blot analysis showed a single mRNA species of 2.1 kb in this protozoan. The mRNA level of GTP cyclohydrolase I increased during synchronous cell division induced by intermittent heat treatment. The results suggest that the mRNA expression is associated with the cell cycle of T. pyriformis. (C) 2000 Elsevier Science Inc.
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U2 - 10.1016/S0305-0491(00)00239-X
DO - 10.1016/S0305-0491(00)00239-X
M3 - Article
C2 - 11126753
AN - SCOPUS:12944273554
SN - 0305-0491
VL - 127
SP - 65
EP - 73
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
IS - 1
ER -