H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency

Akira Kunitomi; Ryoko Hirohata; Mitsujiro Osawa; Kaho Washizu; Vanessa Arreola; Norikazu Saiki; Tomoaki M. Kato; Masaki Nomura; Haruko Kunitomi; Tokiko Ohkame; Yusuke Ohkame; Jitsutaro Kawaguchi; Hiroto Hara; Kohji Kusano; Takuya Yamamoto; Yasuhiro Takashima; Shugo Tohyama; Shinsuke Yuasa; Keiichi Fukuda; Naoko Takasu; Shinya Yamanaka

doi:10.1016/j.stemcr.2024.04.005

H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency

Akira Kunitomi
, Ryoko Hirohata
, Mitsujiro Osawa
, Kaho Washizu
, Vanessa Arreola
, Norikazu Saiki
, Tomoaki M. Kato
, Masaki Nomura
, Haruko Kunitomi
, Tokiko Ohkame
, Yusuke Ohkame
, Jitsutaro Kawaguchi
, Hiroto Hara
, Kohji Kusano
, Takuya Yamamoto
, Yasuhiro Takashima
, Shugo Tohyama
, Shinsuke Yuasa
, Keiichi Fukuda
, Naoko Takasu

Shinya Yamanaka

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Heterogeneity among both primed and naive pluripotent stem cell lines remains a major unresolved problem. Here we show that expressing the maternal-specific linker histone H1FOO fused to a destabilizing domain (H1FOO-DD), together with OCT4, SOX2, KLF4, and LMYC, in human somatic cells improves the quality of reprogramming to both primed and naive pluripotency. H1FOO-DD expression was associated with altered chromatin accessibility around pluripotency genes and with suppression of the innate immune response. Notably, H1FOO-DD generates naive induced pluripotent stem cells with lower variation in transcriptome and methylome among clones and a more uniform and superior differentiation potency. Furthermore, we elucidated that upregulation of FKBP1A, driven by these five factors, plays a key role in H1FOO-DD-mediated reprogramming.

Original language	English
Pages (from-to)	710-728
Number of pages	19
Journal	Stem Cell Reports
Volume	19
Issue number	5
DOIs	https://doi.org/10.1016/j.stemcr.2024.04.005
Publication status	Published - 14-05-2024
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Genetics
Developmental Biology
Cell Biology

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10.1016/j.stemcr.2024.04.005

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Kunitomi, A., Hirohata, R., Osawa, M., Washizu, K., Arreola, V., Saiki, N., Kato, T. M., Nomura, M., Kunitomi, H., Ohkame, T., Ohkame, Y., Kawaguchi, J., Hara, H., Kusano, K., Yamamoto, T., Takashima, Y., Tohyama, S., Yuasa, S., Fukuda, K., ... Yamanaka, S. (2024). H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency. Stem Cell Reports, 19(5), 710-728. https://doi.org/10.1016/j.stemcr.2024.04.005

@article{e62f6b30ef2c4698aba9b27113f2f59b,

title = "H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency",

abstract = "Heterogeneity among both primed and naive pluripotent stem cell lines remains a major unresolved problem. Here we show that expressing the maternal-specific linker histone H1FOO fused to a destabilizing domain (H1FOO-DD), together with OCT4, SOX2, KLF4, and LMYC, in human somatic cells improves the quality of reprogramming to both primed and naive pluripotency. H1FOO-DD expression was associated with altered chromatin accessibility around pluripotency genes and with suppression of the innate immune response. Notably, H1FOO-DD generates naive induced pluripotent stem cells with lower variation in transcriptome and methylome among clones and a more uniform and superior differentiation potency. Furthermore, we elucidated that upregulation of FKBP1A, driven by these five factors, plays a key role in H1FOO-DD-mediated reprogramming.",

keywords = "FKBP1A, H1FOO, Sendai virus vector, destabilized domain, heterogeneity, induced pluripotent stem cell, innate immune response, naive pluripotency, primed pluripotency, reprogramming",

author = "Akira Kunitomi and Ryoko Hirohata and Mitsujiro Osawa and Kaho Washizu and Vanessa Arreola and Norikazu Saiki and Kato, \{Tomoaki M.\} and Masaki Nomura and Haruko Kunitomi and Tokiko Ohkame and Yusuke Ohkame and Jitsutaro Kawaguchi and Hiroto Hara and Kohji Kusano and Takuya Yamamoto and Yasuhiro Takashima and Shugo Tohyama and Shinsuke Yuasa and Keiichi Fukuda and Naoko Takasu and Shinya Yamanaka",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s)",

year = "2024",

month = may,

day = "14",

doi = "10.1016/j.stemcr.2024.04.005",

language = "English",

volume = "19",

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journal = "Stem Cell Reports",

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Kunitomi, A, Hirohata, R, Osawa, M, Washizu, K, Arreola, V, Saiki, N, Kato, TM, Nomura, M, Kunitomi, H, Ohkame, T, Ohkame, Y, Kawaguchi, J, Hara, H, Kusano, K, Yamamoto, T, Takashima, Y, Tohyama, S, Yuasa, S, Fukuda, K, Takasu, N & Yamanaka, S 2024, 'H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency', Stem Cell Reports, vol. 19, no. 5, pp. 710-728. https://doi.org/10.1016/j.stemcr.2024.04.005

TY - JOUR

T1 - H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency

AU - Kunitomi, Akira

AU - Hirohata, Ryoko

AU - Osawa, Mitsujiro

AU - Washizu, Kaho

AU - Arreola, Vanessa

AU - Saiki, Norikazu

AU - Kato, Tomoaki M.

AU - Nomura, Masaki

AU - Kunitomi, Haruko

AU - Ohkame, Tokiko

AU - Ohkame, Yusuke

AU - Kawaguchi, Jitsutaro

AU - Hara, Hiroto

AU - Kusano, Kohji

AU - Yamamoto, Takuya

AU - Takashima, Yasuhiro

AU - Tohyama, Shugo

AU - Yuasa, Shinsuke

AU - Fukuda, Keiichi

AU - Takasu, Naoko

AU - Yamanaka, Shinya

PY - 2024/5/14

Y1 - 2024/5/14

N2 - Heterogeneity among both primed and naive pluripotent stem cell lines remains a major unresolved problem. Here we show that expressing the maternal-specific linker histone H1FOO fused to a destabilizing domain (H1FOO-DD), together with OCT4, SOX2, KLF4, and LMYC, in human somatic cells improves the quality of reprogramming to both primed and naive pluripotency. H1FOO-DD expression was associated with altered chromatin accessibility around pluripotency genes and with suppression of the innate immune response. Notably, H1FOO-DD generates naive induced pluripotent stem cells with lower variation in transcriptome and methylome among clones and a more uniform and superior differentiation potency. Furthermore, we elucidated that upregulation of FKBP1A, driven by these five factors, plays a key role in H1FOO-DD-mediated reprogramming.

AB - Heterogeneity among both primed and naive pluripotent stem cell lines remains a major unresolved problem. Here we show that expressing the maternal-specific linker histone H1FOO fused to a destabilizing domain (H1FOO-DD), together with OCT4, SOX2, KLF4, and LMYC, in human somatic cells improves the quality of reprogramming to both primed and naive pluripotency. H1FOO-DD expression was associated with altered chromatin accessibility around pluripotency genes and with suppression of the innate immune response. Notably, H1FOO-DD generates naive induced pluripotent stem cells with lower variation in transcriptome and methylome among clones and a more uniform and superior differentiation potency. Furthermore, we elucidated that upregulation of FKBP1A, driven by these five factors, plays a key role in H1FOO-DD-mediated reprogramming.

KW - FKBP1A

KW - H1FOO

KW - Sendai virus vector

KW - destabilized domain

KW - heterogeneity

KW - induced pluripotent stem cell

KW - innate immune response

KW - naive pluripotency

KW - primed pluripotency

KW - reprogramming

UR - https://www.scopus.com/pages/publications/85192450424

UR - https://www.scopus.com/pages/publications/85192450424#tab=citedBy

U2 - 10.1016/j.stemcr.2024.04.005

DO - 10.1016/j.stemcr.2024.04.005

M3 - Article

C2 - 38701780

AN - SCOPUS:85192450424

SN - 2213-6711

VL - 19

SP - 710

EP - 728

JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 5

ER -

H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency

Abstract

All Science Journal Classification (ASJC) codes

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