Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method

Ryota Suzuki, Masaru Ihira, Yoshihiko Enomoto, Hiroaki Yano, Fumio Maruyama, Nobuhiko Emi, Yoshizo Asano, Tetsushi Yoshikawa

Research output: Contribution to journalComment/debate

18 Citations (Scopus)

Abstract

A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4%) by the original CMV LAMP procedure and 148 of350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.

Original languageEnglish
Pages (from-to)466-470
Number of pages5
JournalMicrobiology and Immunology
Volume54
Issue number8
DOIs
Publication statusPublished - 01-01-2010

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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