Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method

Ryota Suzuki; Masaru Ihira; Yoshihiko Enomoto; Hiroaki Yano; Fumio Maruyama; Nobuhiko Emi; Yoshizo Asano; Tetsushi Yoshikawa

doi:10.1111/j.1348-0421.2010.00236.x

Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method

Ryota Suzuki
, Masaru Ihira
, Yoshihiko Enomoto
, Hiroaki Yano
, Fumio Maruyama
, Nobuhiko Emi
, Yoshizo Asano
, Tetsushi Yoshikawa

Clinical Science for biological monitoring

Research output: Contribution to journal › Comment/debate › peer-review

28 Citations (Scopus)

Abstract

A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4%) by the original CMV LAMP procedure and 148 of350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.

Original language	English
Pages (from-to)	466-470
Number of pages	5
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	54
Issue number	8
DOIs	https://doi.org/10.1111/j.1348-0421.2010.00236.x
Publication status	Published - 08-2010

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.2010.00236.x

Cite this

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title = "Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method",

abstract = "A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4\%) by the original CMV LAMP procedure and 148 of350 samples (42.3\%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9\%, 77.7\%, 62.2\%, and 96.5\%, respectively.",

keywords = "CMV, Heat denaturation, LAMP",

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T1 - Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method

AU - Suzuki, Ryota

AU - Ihira, Masaru

AU - Enomoto, Yoshihiko

AU - Yano, Hiroaki

AU - Maruyama, Fumio

AU - Emi, Nobuhiko

AU - Asano, Yoshizo

AU - Yoshikawa, Tetsushi

PY - 2010/8

Y1 - 2010/8

N2 - A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4%) by the original CMV LAMP procedure and 148 of350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.

AB - A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of350 samples (29.4%) by the original CMV LAMP procedure and 148 of350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.

KW - CMV

KW - Heat denaturation

KW - LAMP

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DO - 10.1111/j.1348-0421.2010.00236.x

M3 - Comment/debate

C2 - 20646211

AN - SCOPUS:77955000280

SN - 0385-5600

VL - 54

SP - 466

EP - 470

JO - MICROBIOLOGY and IMMUNOLOGY

JF - MICROBIOLOGY and IMMUNOLOGY

IS - 8

ER -

Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method

Abstract

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