Heat shock protein 60 elicits abnormal response in macrophages of diabetes-prone non-obese diabetic mice

Thure Adler, Hidehiko Akiyama, Christian Herder, Hubert Kolb, Volker Burkart

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Heat shock protein 60 (hsp60) is a target antigen in autoimmune diabetes and injections of human hsp60 for tolerance induction were found to protect non-obese diabetic (NOD) mice an animal model of human type 1 diabetes from disease development. We tested whether innate immune cells of NOD mice exhibit an abnormal response to extracellular hsp60. Bone marrow derived macrophages (BMM) were grown from NOD C57BL/6J non-obese non-diabetic (NON) mice and NOD-related congenic variants differing in the Idd-3 Idd-10/18 or major histocompatibility complex (MHC) region. Hsp60-stimulated BMM of NOD mice were found to produce high levels of interleukin (IL)-12(p70). The addition of IL-10 downregulated whereas cyclooxygenase inhibitors elevated IL-12(p70) production of activated BMM. BMM of NON NON-NOD-H-2g7 as well as of NOD-NON-H-2nbl mice produced significantly less IL-12(p70) than BMM of NOD mice indicating that an interaction between the MHC haplotype and non-MHC genes of the NOD mouse is required for hyperresponsiveness to hsp60.

Original languageEnglish
Pages (from-to)592-596
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume294
Issue number3
DOIs
Publication statusPublished - 01-01-2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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