Herpes simplex virus type 2 UL34 protein requires UL31 protein for its relocation to the internal nuclear membrane in transfected cells

Y. Yamauchi, C. Shiba, F. Goshima, A. Nawa, Takayuki Murata, Y. Nishiyama

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Herpes simplex virus type 2 UL34 protein is expressed late in infection and is required for envelopment of nucleocapsids at the nuclear membrane and possibly at the endoplasmic reticulum (ER). It is a type II membrane protein with a C-terminal anchor that localizes mainly to the nuclear membrane in infected cells. However, in single transient expression, UL34 protein localizes predominantly to the ER. Relocation of UL34 protein from the ER to the internal nuclear membrane and the nucleus was observed in the presence of UL31 protein, a phosphoprotein known to interact physically with UL34. It is suggested here that interaction with UL31 protein is important for the nuclear targetting of UL34 protein and also that the trans-membrane region of UL34 protein is responsible for its localization at the internal nuclear membrane. The results also suggest possible sites for the interaction.

Original languageEnglish
Pages (from-to)1423-1428
Number of pages6
JournalJournal of General Virology
Volume82
Issue number6
DOIs
Publication statusPublished - 01-01-2001
Externally publishedYes

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Human Herpesvirus 2
Nuclear Envelope
Endoplasmic Reticulum
Proteins
Nucleocapsid
Phosphoproteins
Protein Transport
Membrane Proteins
Membranes
Infection

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

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title = "Herpes simplex virus type 2 UL34 protein requires UL31 protein for its relocation to the internal nuclear membrane in transfected cells",
abstract = "Herpes simplex virus type 2 UL34 protein is expressed late in infection and is required for envelopment of nucleocapsids at the nuclear membrane and possibly at the endoplasmic reticulum (ER). It is a type II membrane protein with a C-terminal anchor that localizes mainly to the nuclear membrane in infected cells. However, in single transient expression, UL34 protein localizes predominantly to the ER. Relocation of UL34 protein from the ER to the internal nuclear membrane and the nucleus was observed in the presence of UL31 protein, a phosphoprotein known to interact physically with UL34. It is suggested here that interaction with UL31 protein is important for the nuclear targetting of UL34 protein and also that the trans-membrane region of UL34 protein is responsible for its localization at the internal nuclear membrane. The results also suggest possible sites for the interaction.",
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Herpes simplex virus type 2 UL34 protein requires UL31 protein for its relocation to the internal nuclear membrane in transfected cells. / Yamauchi, Y.; Shiba, C.; Goshima, F.; Nawa, A.; Murata, Takayuki; Nishiyama, Y.

In: Journal of General Virology, Vol. 82, No. 6, 01.01.2001, p. 1423-1428.

Research output: Contribution to journalArticle

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AU - Yamauchi, Y.

AU - Shiba, C.

AU - Goshima, F.

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AU - Murata, Takayuki

AU - Nishiyama, Y.

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