Hexane Extract of Citrus sphaerocarpa Suppresses the Accumulation of Lipid Droplets in 3T3-L1 Adipocytes

Takehiko Tsuji; Akihiro Ikuno; Takuya Hayakawa; Hirotaka Katsuzaki; Shin Ichiro Takebayashi; Kazuhiro Kagotani; Katsuzumi Okumura

doi:10.1021/acsfoodscitech.2c00200

Hexane Extract of Citrus sphaerocarpa Suppresses the Accumulation of Lipid Droplets in 3T3-L1 Adipocytes

Takehiko Tsuji
, Akihiro Ikuno
, Takuya Hayakawa
, Hirotaka Katsuzaki
, Shin Ichiro Takebayashi
, Kazuhiro Kagotani
, Katsuzumi Okumura

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

In this study, the hexane extract of Citrus sphaerocarpa (CSHE) was demonstrated in a dose-dependent manner to suppress the accumulation of lipid droplets in 3T3-L1 adipocytes. Furthermore, RNA-Seq analysis showed that CSHE treatment affected the expression of 1180 genes that are associated with adipocyte differentiation. Information on gene expression indicated that CSHE treatment may suppress adipogenesis because of the down- and upregulation of glycolysis/gluconeogenesis and β-oxidation, respectively, via insulin, PI3K/Akt, PKA/AMPK, and glucagon signaling pathways. In addition, the elution fraction of n-hexane/ethanol = 9:1 (v/v) from CSHE using silica gel column chromatography contained active components. Liquid chromatography/mass spectrometry (LC/MS) analysis of this fraction revealed the presence of polymethoxyflavones (PMFs) with hydroxyl groups. Based on previous reports, we hypothesize that hydroxylated PMFs have a particularly strong anti-obesity effect among the PMFs contained in citrus fruits.

Original language	English
Pages (from-to)	1507-1516
Number of pages	10
Journal	ACS Food Science and Technology
Volume	2
Issue number	9
DOIs	https://doi.org/10.1021/acsfoodscitech.2c00200
Publication status	Published - 16-09-2022
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Food Science
Chemistry (miscellaneous)
Organic Chemistry

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10.1021/acsfoodscitech.2c00200

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title = "Hexane Extract of Citrus sphaerocarpa Suppresses the Accumulation of Lipid Droplets in 3T3-L1 Adipocytes",

abstract = "In this study, the hexane extract of Citrus sphaerocarpa (CSHE) was demonstrated in a dose-dependent manner to suppress the accumulation of lipid droplets in 3T3-L1 adipocytes. Furthermore, RNA-Seq analysis showed that CSHE treatment affected the expression of 1180 genes that are associated with adipocyte differentiation. Information on gene expression indicated that CSHE treatment may suppress adipogenesis because of the down- and upregulation of glycolysis/gluconeogenesis and β-oxidation, respectively, via insulin, PI3K/Akt, PKA/AMPK, and glucagon signaling pathways. In addition, the elution fraction of n-hexane/ethanol = 9:1 (v/v) from CSHE using silica gel column chromatography contained active components. Liquid chromatography/mass spectrometry (LC/MS) analysis of this fraction revealed the presence of polymethoxyflavones (PMFs) with hydroxyl groups. Based on previous reports, we hypothesize that hydroxylated PMFs have a particularly strong anti-obesity effect among the PMFs contained in citrus fruits.",

keywords = "Citrus sphaerocarpa, hydroxylated polymethoxyflavones, Obesity, RNA-Seq method",

author = "Takehiko Tsuji and Akihiro Ikuno and Takuya Hayakawa and Hirotaka Katsuzaki and Takebayashi, \{Shin Ichiro\} and Kazuhiro Kagotani and Katsuzumi Okumura",

note = "Publisher Copyright: {\textcopyright} 2022 American Chemical Society.",

year = "2022",

month = sep,

day = "16",

doi = "10.1021/acsfoodscitech.2c00200",

language = "English",

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T1 - Hexane Extract of Citrus sphaerocarpa Suppresses the Accumulation of Lipid Droplets in 3T3-L1 Adipocytes

AU - Tsuji, Takehiko

AU - Ikuno, Akihiro

AU - Hayakawa, Takuya

AU - Katsuzaki, Hirotaka

AU - Takebayashi, Shin Ichiro

AU - Kagotani, Kazuhiro

AU - Okumura, Katsuzumi

PY - 2022/9/16

Y1 - 2022/9/16

N2 - In this study, the hexane extract of Citrus sphaerocarpa (CSHE) was demonstrated in a dose-dependent manner to suppress the accumulation of lipid droplets in 3T3-L1 adipocytes. Furthermore, RNA-Seq analysis showed that CSHE treatment affected the expression of 1180 genes that are associated with adipocyte differentiation. Information on gene expression indicated that CSHE treatment may suppress adipogenesis because of the down- and upregulation of glycolysis/gluconeogenesis and β-oxidation, respectively, via insulin, PI3K/Akt, PKA/AMPK, and glucagon signaling pathways. In addition, the elution fraction of n-hexane/ethanol = 9:1 (v/v) from CSHE using silica gel column chromatography contained active components. Liquid chromatography/mass spectrometry (LC/MS) analysis of this fraction revealed the presence of polymethoxyflavones (PMFs) with hydroxyl groups. Based on previous reports, we hypothesize that hydroxylated PMFs have a particularly strong anti-obesity effect among the PMFs contained in citrus fruits.

AB - In this study, the hexane extract of Citrus sphaerocarpa (CSHE) was demonstrated in a dose-dependent manner to suppress the accumulation of lipid droplets in 3T3-L1 adipocytes. Furthermore, RNA-Seq analysis showed that CSHE treatment affected the expression of 1180 genes that are associated with adipocyte differentiation. Information on gene expression indicated that CSHE treatment may suppress adipogenesis because of the down- and upregulation of glycolysis/gluconeogenesis and β-oxidation, respectively, via insulin, PI3K/Akt, PKA/AMPK, and glucagon signaling pathways. In addition, the elution fraction of n-hexane/ethanol = 9:1 (v/v) from CSHE using silica gel column chromatography contained active components. Liquid chromatography/mass spectrometry (LC/MS) analysis of this fraction revealed the presence of polymethoxyflavones (PMFs) with hydroxyl groups. Based on previous reports, we hypothesize that hydroxylated PMFs have a particularly strong anti-obesity effect among the PMFs contained in citrus fruits.

KW - Citrus sphaerocarpa

KW - hydroxylated polymethoxyflavones

KW - Obesity

KW - RNA-Seq method

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U2 - 10.1021/acsfoodscitech.2c00200

DO - 10.1021/acsfoodscitech.2c00200

M3 - Article

AN - SCOPUS:85138179853

SN - 2692-1944

VL - 2

SP - 1507

EP - 1516

JO - ACS Food Science and Technology

JF - ACS Food Science and Technology

IS - 9

ER -

Hexane Extract of Citrus sphaerocarpa Suppresses the Accumulation of Lipid Droplets in 3T3-L1 Adipocytes

Abstract

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