TY - JOUR
T1 - HF10, an attenuated herpes simplex virus (HSV) type 1 clone, lacks neuroinvasiveness and protects mice against lethal challenge with HSV types 1 and 2
AU - Mori, Isamu
AU - Liu, Beixing
AU - Goshima, Fumi
AU - Ito, Hiroyasu
AU - Koide, Naoki
AU - Yoshida, Tomoaki
AU - Yokochi, Takashi
AU - Kimura, Yoshinobu
AU - Nishiyama, Yukihiro
N1 - Funding Information:
We thank Professor S. Kikuchi for helpful advice on statistic analyses and E. Iwata, and T. Tsuruguchi for technical assistance. This work was supported by a Grant-in-Aid for Science Research of Japan Society of the Promotion of Science (JSPS) (15590424). I. Mori is the recipient of an Encouragement of Young Scientist Award (2004) from the Nakanihon Infectious Diseases Research Foundation, Japan.
PY - 2005/12
Y1 - 2005/12
N2 - Herpes simplex virus (HSV), a neurotropic virus, establishes life-long and, although rare, life-threatening infection in humans, and it may precipitate substantial medical and psychosocial morbidity. Here we show that HSV-1 strain HF clone 10 (HF10) exhibits impaired neuroinvasiveness in peripheral olfactory, vomeronasal and trigeminal conduits following intranasal as well as corneal inoculation. HF10 attenuation likely arises from multiple defects of HSV genes, so that HF10 will not revert to a virulent phenotype. Intranasal vaccination of mice with HF10 conferred significant protection against lethal challenge with HSV-1 and HSV-2 via the intranasal and intravaginal routes. Thus, we propose that HF10 explicitly meets the prerequisites for a candidate live attenuated HSV vaccine.
AB - Herpes simplex virus (HSV), a neurotropic virus, establishes life-long and, although rare, life-threatening infection in humans, and it may precipitate substantial medical and psychosocial morbidity. Here we show that HSV-1 strain HF clone 10 (HF10) exhibits impaired neuroinvasiveness in peripheral olfactory, vomeronasal and trigeminal conduits following intranasal as well as corneal inoculation. HF10 attenuation likely arises from multiple defects of HSV genes, so that HF10 will not revert to a virulent phenotype. Intranasal vaccination of mice with HF10 conferred significant protection against lethal challenge with HSV-1 and HSV-2 via the intranasal and intravaginal routes. Thus, we propose that HF10 explicitly meets the prerequisites for a candidate live attenuated HSV vaccine.
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U2 - 10.1016/j.micinf.2005.05.007
DO - 10.1016/j.micinf.2005.05.007
M3 - Article
C2 - 16054416
AN - SCOPUS:28944445461
SN - 1286-4579
VL - 7
SP - 1492
EP - 1500
JO - Microbes and Infection
JF - Microbes and Infection
IS - 15
ER -
