TY - JOUR
T1 - High affinity D- and L-serine transporter Asc-1
T2 - Cloning and dendritic localization in the rat cerebral and cerebellar cortices
AU - Matsuo, Hirotaka
AU - Kanai, Yoshikatsu
AU - Tokunaga, Motohide
AU - Nakata, Takahiro
AU - Chairoungdua, Arthit
AU - Ishimine, Hisako
AU - Tsukada, Shingo
AU - Ooigawa, Hidetoshi
AU - Nawashiro, Hiroshi
AU - Kobayashi, Yasushi
AU - Fukuda, Jun
AU - Endou, Hitoshi
N1 - Funding Information:
This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan, the Japan Society for the Promotion of Science, the Promotion and Mutual Aid Corporation for Private Schools of Japan, the Japan Health Sciences Foundation (to Y.K. and H.E.), Uehara Memorial Foundation, YASUDA Medical Research Foundation (to Y.K.), Health and Labour Sciences Research Grants for Research on Advanced Medical Technology: Toxicogenomics Project (to H.E.), and Kowa Life Sciences Foundation (to H.M.). The authors also thank Michi Takahashi, Ryo Kinoshita, Akiyoshi Nakayama and Satoru Watanabe for technical assistance. Anti-Asc-1 antibodies were supplied by Kumamoto Immunochemical Laboratory, Transgenic Inc., Kumamoto, Japan. The nucleotide sequence for rAsc-1 has been submitted to the GenBank/EMBL/DDBJ Data Bank with an accession number AB126813 .
PY - 2004/3/25
Y1 - 2004/3/25
N2 - System asc transporter Asc-1, expressed in the brain, transports D- and L-serine with high affinity. To determine the localization of Asc-1 in the rat brain, we isolated a cDNA for the rat orthologue of Asc-1. The encoded protein designated as rAsc-1 (rat Asc-1) exhibited 98% sequence identity to mouse Asc-1 (mAsc-1). Based on amino acid sequences of rAsc-1 and mAsc-1, two polyclonal antibodies against Asc-1 were generated and used for the immunohistochemical analysis on the cerebral and cerebellar cortices of rats and mice. Asc-1 immunoreactivity was detected in neurons, including cerebellar Purkinje neurons and pyramidal neurons in the neocortex and hippocampus. It was clearly localized in dendrites as well as somata. The localization of Asc-1 in brain suggests the significant contribution of Asc-1 to amino acid mobilization in brains including the synaptic clearance of D-serine and the neuronal uptake of L-serine that is essential for survival and dendrite growth of Purkinje neurons in particular.
AB - System asc transporter Asc-1, expressed in the brain, transports D- and L-serine with high affinity. To determine the localization of Asc-1 in the rat brain, we isolated a cDNA for the rat orthologue of Asc-1. The encoded protein designated as rAsc-1 (rat Asc-1) exhibited 98% sequence identity to mouse Asc-1 (mAsc-1). Based on amino acid sequences of rAsc-1 and mAsc-1, two polyclonal antibodies against Asc-1 were generated and used for the immunohistochemical analysis on the cerebral and cerebellar cortices of rats and mice. Asc-1 immunoreactivity was detected in neurons, including cerebellar Purkinje neurons and pyramidal neurons in the neocortex and hippocampus. It was clearly localized in dendrites as well as somata. The localization of Asc-1 in brain suggests the significant contribution of Asc-1 to amino acid mobilization in brains including the synaptic clearance of D-serine and the neuronal uptake of L-serine that is essential for survival and dendrite growth of Purkinje neurons in particular.
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U2 - 10.1016/j.neulet.2004.01.014
DO - 10.1016/j.neulet.2004.01.014
M3 - Article
C2 - 15026164
AN - SCOPUS:12144289325
SN - 0304-3940
VL - 358
SP - 123
EP - 126
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -