High affinity D- and L-serine transporter Asc-1: Cloning and dendritic localization in the rat cerebral and cerebellar cortices

Hirotaka Matsuo, Yoshikatsu Kanai, Motohide Tokunaga, Takahiro Nakata, Arthit Chairoungdua, Hisako Ishimine, Shingo Tsukada, Hidetoshi Ooigawa, Hiroshi Nawashiro, Yasushi Kobayashi, Jun Fukuda, Hitoshi Endou

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

System asc transporter Asc-1, expressed in the brain, transports D- and L-serine with high affinity. To determine the localization of Asc-1 in the rat brain, we isolated a cDNA for the rat orthologue of Asc-1. The encoded protein designated as rAsc-1 (rat Asc-1) exhibited 98% sequence identity to mouse Asc-1 (mAsc-1). Based on amino acid sequences of rAsc-1 and mAsc-1, two polyclonal antibodies against Asc-1 were generated and used for the immunohistochemical analysis on the cerebral and cerebellar cortices of rats and mice. Asc-1 immunoreactivity was detected in neurons, including cerebellar Purkinje neurons and pyramidal neurons in the neocortex and hippocampus. It was clearly localized in dendrites as well as somata. The localization of Asc-1 in brain suggests the significant contribution of Asc-1 to amino acid mobilization in brains including the synaptic clearance of D-serine and the neuronal uptake of L-serine that is essential for survival and dendrite growth of Purkinje neurons in particular.

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalNeuroscience Letters
Volume358
Issue number2
DOIs
Publication statusPublished - 25-03-2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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