High mobility group box chromosomal protein 1 in patients with renal diseases

Fumihiko Sato, Shoichi Maruyama, Hiroki Hayashi, Izumi Sakamoto, Shingo Yamada, Tomonori Uchimura, Yoshiki Morita, Yasuhiko Ito, Yukio Yuzawa, Ikuro Maruyama, Seiichi Matsuo

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24 Citations (Scopus)

Abstract

Background/Aim: The high mobility group box chromosomal protein 1 (HMGB1), a nuclear DNA-binding protein, has recently been recognized as a new proinflammatory cytokine. The purpose of this study was to examine the significance of HMGB1 in patients with renal diseases. Methods: HMGB1 concentrations in sera were measured by enzyme-linked immunosorbent assay, and antibodies against HMGB1 were examined by Western blotting in patients who underwent renal biopsies and in healthy controls. Immunohistochemistry for HMGB1 was also performed. Results: Serum HMGB1 was more likely to be positive in patients who underwent renal biopsies as compared with the controls. Patients with anti-neutrophil cytoplasmic antibody-related glomerulonephritis (ANCA-GN) and those with Henoch-Schönlein purpura nephritis showed a significantly higher tendency to be HMGB1 positive. The presence of anti-HMGB1 antibody was not associated with the presence of serum HMGB1. Immunohistochemistry revealed that HMGB1 was expressed in mononuclear cells in the interstitium or in the glomeruli of some patients with ANCA-GN or IgA nephropathy (IgAN). Subanalysis demonstrated that among patients with IgAN, those who had crescent formation showed a higher tendency to be HMGB1 positive than those who did not. Conclusions: HMGB1 was expressed in the sera of patients with renal diseases who underwent renal biopsies, especially among those who had vasculitis including ANCA-GN, Henoch-Schönlein purpura nephritis, and IgAN with glomerular crescents.

Original languageEnglish
JournalNephron - Clinical Practice
Volume108
Issue number3
DOIs
Publication statusPublished - 01-04-2008
Externally publishedYes

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High Mobility Group Proteins
HMGB1 Protein
Kidney
Antineutrophil Cytoplasmic Antibodies
Glomerulonephritis
Immunoglobulin A
Schoenlein-Henoch Purpura
Nephritis
Serum
Biopsy
Immunohistochemistry
Antibodies
DNA-Binding Proteins
Nuclear Proteins
Vasculitis

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Sato, Fumihiko ; Maruyama, Shoichi ; Hayashi, Hiroki ; Sakamoto, Izumi ; Yamada, Shingo ; Uchimura, Tomonori ; Morita, Yoshiki ; Ito, Yasuhiko ; Yuzawa, Yukio ; Maruyama, Ikuro ; Matsuo, Seiichi. / High mobility group box chromosomal protein 1 in patients with renal diseases. In: Nephron - Clinical Practice. 2008 ; Vol. 108, No. 3.
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abstract = "Background/Aim: The high mobility group box chromosomal protein 1 (HMGB1), a nuclear DNA-binding protein, has recently been recognized as a new proinflammatory cytokine. The purpose of this study was to examine the significance of HMGB1 in patients with renal diseases. Methods: HMGB1 concentrations in sera were measured by enzyme-linked immunosorbent assay, and antibodies against HMGB1 were examined by Western blotting in patients who underwent renal biopsies and in healthy controls. Immunohistochemistry for HMGB1 was also performed. Results: Serum HMGB1 was more likely to be positive in patients who underwent renal biopsies as compared with the controls. Patients with anti-neutrophil cytoplasmic antibody-related glomerulonephritis (ANCA-GN) and those with Henoch-Sch{\"o}nlein purpura nephritis showed a significantly higher tendency to be HMGB1 positive. The presence of anti-HMGB1 antibody was not associated with the presence of serum HMGB1. Immunohistochemistry revealed that HMGB1 was expressed in mononuclear cells in the interstitium or in the glomeruli of some patients with ANCA-GN or IgA nephropathy (IgAN). Subanalysis demonstrated that among patients with IgAN, those who had crescent formation showed a higher tendency to be HMGB1 positive than those who did not. Conclusions: HMGB1 was expressed in the sera of patients with renal diseases who underwent renal biopsies, especially among those who had vasculitis including ANCA-GN, Henoch-Sch{\"o}nlein purpura nephritis, and IgAN with glomerular crescents.",
author = "Fumihiko Sato and Shoichi Maruyama and Hiroki Hayashi and Izumi Sakamoto and Shingo Yamada and Tomonori Uchimura and Yoshiki Morita and Yasuhiko Ito and Yukio Yuzawa and Ikuro Maruyama and Seiichi Matsuo",
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Sato, F, Maruyama, S, Hayashi, H, Sakamoto, I, Yamada, S, Uchimura, T, Morita, Y, Ito, Y, Yuzawa, Y, Maruyama, I & Matsuo, S 2008, 'High mobility group box chromosomal protein 1 in patients with renal diseases', Nephron - Clinical Practice, vol. 108, no. 3. https://doi.org/10.1159/000118942

High mobility group box chromosomal protein 1 in patients with renal diseases. / Sato, Fumihiko; Maruyama, Shoichi; Hayashi, Hiroki; Sakamoto, Izumi; Yamada, Shingo; Uchimura, Tomonori; Morita, Yoshiki; Ito, Yasuhiko; Yuzawa, Yukio; Maruyama, Ikuro; Matsuo, Seiichi.

In: Nephron - Clinical Practice, Vol. 108, No. 3, 01.04.2008.

Research output: Contribution to journalArticle

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AU - Sato, Fumihiko

AU - Maruyama, Shoichi

AU - Hayashi, Hiroki

AU - Sakamoto, Izumi

AU - Yamada, Shingo

AU - Uchimura, Tomonori

AU - Morita, Yoshiki

AU - Ito, Yasuhiko

AU - Yuzawa, Yukio

AU - Maruyama, Ikuro

AU - Matsuo, Seiichi

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N2 - Background/Aim: The high mobility group box chromosomal protein 1 (HMGB1), a nuclear DNA-binding protein, has recently been recognized as a new proinflammatory cytokine. The purpose of this study was to examine the significance of HMGB1 in patients with renal diseases. Methods: HMGB1 concentrations in sera were measured by enzyme-linked immunosorbent assay, and antibodies against HMGB1 were examined by Western blotting in patients who underwent renal biopsies and in healthy controls. Immunohistochemistry for HMGB1 was also performed. Results: Serum HMGB1 was more likely to be positive in patients who underwent renal biopsies as compared with the controls. Patients with anti-neutrophil cytoplasmic antibody-related glomerulonephritis (ANCA-GN) and those with Henoch-Schönlein purpura nephritis showed a significantly higher tendency to be HMGB1 positive. The presence of anti-HMGB1 antibody was not associated with the presence of serum HMGB1. Immunohistochemistry revealed that HMGB1 was expressed in mononuclear cells in the interstitium or in the glomeruli of some patients with ANCA-GN or IgA nephropathy (IgAN). Subanalysis demonstrated that among patients with IgAN, those who had crescent formation showed a higher tendency to be HMGB1 positive than those who did not. Conclusions: HMGB1 was expressed in the sera of patients with renal diseases who underwent renal biopsies, especially among those who had vasculitis including ANCA-GN, Henoch-Schönlein purpura nephritis, and IgAN with glomerular crescents.

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