High-performance liquid chromatographic assay for prostaglandin E1 in various ointment vehicles. Separation and stability testing

Keiko Yamamura, Toshihisa Yotsuyanagi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A high-performance liquid chromatographic method for the prostaglandin E1 (PGE1) incorporated in white petrolatum, white ointment, hydrophilic petrolatum and Plastibase was investigated. The prostaglandin was separated from the oleaginous vehicles with n-hexane-aqueous acetonitrile. Most of white petrolatum, which is a principal component in the vehicles, remained in the n-hexane layer, and the recovery of the drug from any vehicle attained 100%. The method was applied to stability studies of PGE1 in white petrolatum and macrogol ointment. In which pure PGE1 and PGE1-α-cyclodextrin complex (PGE1-CD) were incorporated. The drug remained intact for up to 6 months when stored at 5°C. At 25 and 40°C, pure PGE1 was more stable than PGE1-CD and both PGE1 species were more stable in white petrolatum than in macrogol ointment.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalJournal of Chromatography A
Volume303
Issue numberC
DOIs
Publication statusPublished - 1984
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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