High-performance liquid chromatographic assay of anti-inflammatory drugs incorporated in gel ointments Separation and stability testing

Keiko Yamamura, Jun Ichiro Yamada, Toshihisa Yotsuyanagi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

High-performance liquid chromatographic determinations of gabexate mesilate (FOY) prostaglandin E1 (PGE1, PGE1-α-cyclodextrin (PGE1,-CD), prednisolone (PD) and butyl flufenamate (BF) incorporated in gel ointment were investigated. The gel ointment is composed of a carboxy vinyl polymer (1.3%, w/w) and a large amount of an aqueous organic solvent. A methanol extraction system offered simultaneous advantages of the removal of the polymer and the recovery of active ingredients from the gel phase. The recoveries of the drugs were 100%. The stabilities of PGE1, PGE1-CD, FOY, PD and BF incorporated in gel ointment, stored at 5, 25 and 40°C for up to 90 days, were investigated.

Original languageEnglish
Pages (from-to)383-388
Number of pages6
JournalJournal of Chromatography A
Volume331
Issue numberC
DOIs
Publication statusPublished - 1985
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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