High-performance liquid chromatographic determination of diacetylpolyamines using benzoyl chloride as a derivatizing agent: Application to human urine

K. Shimpo, Takeshi Chihara, C. Ida, K. Kawai, K. Wakamatsu, S. Ito, T. Matsui, M. Hirano, T. Watanabe, T. Nagatsu, H. Kuzuya

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We developed a simple reversed-phase high-performance liquid chromatographic method, using pre-column derivatization with benzoyl chloride and ultraviolet detection, for the determination of N1,N12-diacetylspermine in human urine. N1,N12-diacetylspermine in the urine of 42 healthy subjects, 86 non-cancer patients, 11 pregnant women and 104 cancer patients (82 blood cancers and 22 solid cancers) were determined by our method. When 20-μ1 aliquots (equivalent to 50 μ1 of original urine) were injected into HPLC, N1,N12-diacetylspermine was detected in only one (2.4%) of the healthy subjects and in 4(4.7%) of the non-cancer patients, but was detected in all of the pregnant women. In cancer patients, urinary N1,N12-diacetuylspermine was detected in 39 cases (47.6%) of blood cancer and in 8 cases (36.4%) of solid cancer. In addition, we also measured urinary N1,N8-diacetylspermidine in a proportion of the healthy subjects and cancer patients using our HPLC method with minor modifications. Finally, free, monoacetylated and diacetylated polyamines were determined in the urine of a woman during pregnancy, and our results were consistent with those reported by Van den Berg et al. (1986).

Original languageEnglish
Pages (from-to)211-223
Number of pages13
JournalBiogenic Amines
Volume16
Issue number3
Publication statusPublished - 01-01-2001

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Urine
Neoplasms
Healthy Volunteers
Pregnant Women
High Pressure Liquid Chromatography
Polyamines
benzoyl chloride
Pregnancy
N',N''-diacetylspermine

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Pharmacology

Cite this

Shimpo, K. ; Chihara, Takeshi ; Ida, C. ; Kawai, K. ; Wakamatsu, K. ; Ito, S. ; Matsui, T. ; Hirano, M. ; Watanabe, T. ; Nagatsu, T. ; Kuzuya, H. / High-performance liquid chromatographic determination of diacetylpolyamines using benzoyl chloride as a derivatizing agent : Application to human urine. In: Biogenic Amines. 2001 ; Vol. 16, No. 3. pp. 211-223.
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Shimpo, K, Chihara, T, Ida, C, Kawai, K, Wakamatsu, K, Ito, S, Matsui, T, Hirano, M, Watanabe, T, Nagatsu, T & Kuzuya, H 2001, 'High-performance liquid chromatographic determination of diacetylpolyamines using benzoyl chloride as a derivatizing agent: Application to human urine', Biogenic Amines, vol. 16, no. 3, pp. 211-223.

High-performance liquid chromatographic determination of diacetylpolyamines using benzoyl chloride as a derivatizing agent : Application to human urine. / Shimpo, K.; Chihara, Takeshi; Ida, C.; Kawai, K.; Wakamatsu, K.; Ito, S.; Matsui, T.; Hirano, M.; Watanabe, T.; Nagatsu, T.; Kuzuya, H.

In: Biogenic Amines, Vol. 16, No. 3, 01.01.2001, p. 211-223.

Research output: Contribution to journalArticle

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T1 - High-performance liquid chromatographic determination of diacetylpolyamines using benzoyl chloride as a derivatizing agent

T2 - Application to human urine

AU - Shimpo, K.

AU - Chihara, Takeshi

AU - Ida, C.

AU - Kawai, K.

AU - Wakamatsu, K.

AU - Ito, S.

AU - Matsui, T.

AU - Hirano, M.

AU - Watanabe, T.

AU - Nagatsu, T.

AU - Kuzuya, H.

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