TY - JOUR
T1 - High prevalence and phylogenetic analysis of TT-virus infection in Mongolia
AU - Kato, Hideaki
AU - Mizokami, Masashi
AU - Nakano, Tatsunori
AU - Kondo, Yutaka
AU - Dashnyam, Bumbein
AU - Oyunsuren, Tsendsuren
AU - Ueda, Ryuzo
N1 - Funding Information:
M.M. was supported in part by the Japanese Ministry of Education, Science, Sports and Culture (Grant-in-Aid for Scientific Research 06670571), by the Japanese Ministry of Health and Welfare, Health Science Research Grants (Non-A, Non-B Hepatitis Research Grants), from the Japanese International Cooperation Agency, and by the Viral Hepatitis Research Foundation of Japan. The nucleotide sequences data reported in this paper obtained from DDBJ/EMBL/GenBank nucleotide sequence database (accession numbers, AB017881, AB017885, AB017892-3, AB020060, AB020166-85, AF079541-3).
PY - 1999/4
Y1 - 1999/4
N2 - A novel DNA virus, TT-virus (TTV), was isolated from a post-transfusion hepatitis patient in Japan. The prevalence of TTV infection was investigated among patients with chronic liver disease and normal alanine aminotransferase (ALT) volunteers as controls in Mongolia. Polymerase chain reaction (PCR) was employed to detect TTV DNA using specific primers derived from open reading frame 1 (ORF1) of the TTV genome. Nucleotide sequences of samples positive for TTV DNA were determined. The sequences were analyzed by a molecular evolutionary method. Fifty (60.2%) hepatitis patients and 12 (42.9%) volunteers were positive for TTV DNA. The serum ALT levels did not differ significantly between patients with single TTV infection and without TTV, HBV and HCV infection. Similarly, the serum ALT levels did not differ significantly between controls with and without TTV infection. Dual infection of TTV with either HBV or HCV did not affect the ALT levels of hepatitis patients. The molecular evolutionary tree showed that TTV was a heterogeneous virus and all strains could be divided into three genotypes in Mongolia. A new genotype was identified that was distinct from those previously reported. Copyright (C) 1999 Elsevier Science B.V.
AB - A novel DNA virus, TT-virus (TTV), was isolated from a post-transfusion hepatitis patient in Japan. The prevalence of TTV infection was investigated among patients with chronic liver disease and normal alanine aminotransferase (ALT) volunteers as controls in Mongolia. Polymerase chain reaction (PCR) was employed to detect TTV DNA using specific primers derived from open reading frame 1 (ORF1) of the TTV genome. Nucleotide sequences of samples positive for TTV DNA were determined. The sequences were analyzed by a molecular evolutionary method. Fifty (60.2%) hepatitis patients and 12 (42.9%) volunteers were positive for TTV DNA. The serum ALT levels did not differ significantly between patients with single TTV infection and without TTV, HBV and HCV infection. Similarly, the serum ALT levels did not differ significantly between controls with and without TTV infection. Dual infection of TTV with either HBV or HCV did not affect the ALT levels of hepatitis patients. The molecular evolutionary tree showed that TTV was a heterogeneous virus and all strains could be divided into three genotypes in Mongolia. A new genotype was identified that was distinct from those previously reported. Copyright (C) 1999 Elsevier Science B.V.
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U2 - 10.1016/S0168-1702(99)00016-7
DO - 10.1016/S0168-1702(99)00016-7
M3 - Article
C2 - 10392725
AN - SCOPUS:0033043154
SN - 0168-1702
VL - 60
SP - 171
EP - 179
JO - Virus Research
JF - Virus Research
IS - 2
ER -
