High prevalence of equine-like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

Ratana Tacharoenmuang, Satoshi Komoto, Ratigorn Guntapong, Sompong Upachai, Phakapun Singchai, Tomihiko Ide, Saori Fukuda, Kriangsak Ruchusatsawast, Busarawan Sriwantana, Masashi Tatsumi, Kazushi Motomura, Naokazu Takeda, Takayuki Murata, Somchai Sangkitporn, Koki Taniguchi, Tetsushi Yoshikawa

Research output: Contribution to journalArticle

Abstract

Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5%) stool specimens. G1P[8] (44.7%) was the most predominant genotype, followed by G3P[8] (33.7%), G2P[4] (11.5%), G8P[8] (7.0%), and G9P[8] (1.3%). Unusual G3P[9] (0.8%), G3P[10] (0.4%), G4P[6] (0.4%), and G10P[14] (0.2%) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4%), in 2014 decreased to 6.1% in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5% in 2014 to 65.3% in 2015 and 89.8% in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4%) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine-like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine-like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine-like and the other human-like. Thus, we found that characteristic equine-like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.

Original languageEnglish
JournalJournal of Medical Virology
DOIs
Publication statusAccepted/In press - 01-01-2019

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Rotavirus
Gastroenteritis
Thailand
Horses
RNA
Genotype
Genes
Polyacrylamide Gel Electrophoresis
Genome

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Tacharoenmuang, Ratana ; Komoto, Satoshi ; Guntapong, Ratigorn ; Upachai, Sompong ; Singchai, Phakapun ; Ide, Tomihiko ; Fukuda, Saori ; Ruchusatsawast, Kriangsak ; Sriwantana, Busarawan ; Tatsumi, Masashi ; Motomura, Kazushi ; Takeda, Naokazu ; Murata, Takayuki ; Sangkitporn, Somchai ; Taniguchi, Koki ; Yoshikawa, Tetsushi. / High prevalence of equine-like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand. In: Journal of Medical Virology. 2019.
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title = "High prevalence of equine-like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand",
abstract = "Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5{\%}) stool specimens. G1P[8] (44.7{\%}) was the most predominant genotype, followed by G3P[8] (33.7{\%}), G2P[4] (11.5{\%}), G8P[8] (7.0{\%}), and G9P[8] (1.3{\%}). Unusual G3P[9] (0.8{\%}), G3P[10] (0.4{\%}), G4P[6] (0.4{\%}), and G10P[14] (0.2{\%}) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4{\%}), in 2014 decreased to 6.1{\%} in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5{\%} in 2014 to 65.3{\%} in 2015 and 89.8{\%} in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4{\%}) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine-like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine-like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine-like and the other human-like. Thus, we found that characteristic equine-like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.",
author = "Ratana Tacharoenmuang and Satoshi Komoto and Ratigorn Guntapong and Sompong Upachai and Phakapun Singchai and Tomihiko Ide and Saori Fukuda and Kriangsak Ruchusatsawast and Busarawan Sriwantana and Masashi Tatsumi and Kazushi Motomura and Naokazu Takeda and Takayuki Murata and Somchai Sangkitporn and Koki Taniguchi and Tetsushi Yoshikawa",
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Tacharoenmuang, R, Komoto, S, Guntapong, R, Upachai, S, Singchai, P, Ide, T, Fukuda, S, Ruchusatsawast, K, Sriwantana, B, Tatsumi, M, Motomura, K, Takeda, N, Murata, T, Sangkitporn, S, Taniguchi, K & Yoshikawa, T 2019, 'High prevalence of equine-like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand', Journal of Medical Virology. https://doi.org/10.1002/jmv.25591

High prevalence of equine-like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand. / Tacharoenmuang, Ratana; Komoto, Satoshi; Guntapong, Ratigorn; Upachai, Sompong; Singchai, Phakapun; Ide, Tomihiko; Fukuda, Saori; Ruchusatsawast, Kriangsak; Sriwantana, Busarawan; Tatsumi, Masashi; Motomura, Kazushi; Takeda, Naokazu; Murata, Takayuki; Sangkitporn, Somchai; Taniguchi, Koki; Yoshikawa, Tetsushi.

In: Journal of Medical Virology, 01.01.2019.

Research output: Contribution to journalArticle

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T1 - High prevalence of equine-like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

AU - Tacharoenmuang, Ratana

AU - Komoto, Satoshi

AU - Guntapong, Ratigorn

AU - Upachai, Sompong

AU - Singchai, Phakapun

AU - Ide, Tomihiko

AU - Fukuda, Saori

AU - Ruchusatsawast, Kriangsak

AU - Sriwantana, Busarawan

AU - Tatsumi, Masashi

AU - Motomura, Kazushi

AU - Takeda, Naokazu

AU - Murata, Takayuki

AU - Sangkitporn, Somchai

AU - Taniguchi, Koki

AU - Yoshikawa, Tetsushi

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N2 - Group A rotavirus (RVA) is a major cause of acute gastroenteritis in infants and young children worldwide. This study aims to clarify the distribution of G/P types and genetic characteristics of RVAs circulating in Thailand. Between January 2014 and September 2016, 1867 stool specimens were collected from children and adults with acute gastroenteritis in six provinces in Thailand. RVAs were detected in 514/1867 (27.5%) stool specimens. G1P[8] (44.7%) was the most predominant genotype, followed by G3P[8] (33.7%), G2P[4] (11.5%), G8P[8] (7.0%), and G9P[8] (1.3%). Unusual G3P[9] (0.8%), G3P[10] (0.4%), G4P[6] (0.4%), and G10P[14] (0.2%) were also detected at low frequencies. The predominant genotype, G1P[8] (64.4%), in 2014 decreased to 6.1% in 2016. In contrast, the frequency of G3P[8] markedly increased from 5.5% in 2014 to 65.3% in 2015 and 89.8% in 2016. On polyacrylamide gel electrophoresis, most (135/140; 96.4%) of the G3P[8] strains exhibited a short RNA profile. Successful determination of the nucleotide sequences of the VP7 genes of 98 G3P[8] strains with a short RNA profile showed that they are all equine-like G3P[8] strains. On phylogenetic analysis of genome segments of two representative Thai equine-like G3P[8] strains, it was noteworthy that they possessed distinct NSP4 genes, one bovine-like and the other human-like. Thus, we found that characteristic equine-like G3P[8] strains with a short RNA electropherotype are becoming highly prevalent in children and adults in Thailand.

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