Highly sensitive ELISA for soluble Fas in serum

Increased soluble Fas in the elderly

Mitsuru Seishima, Masao Takemura, Kuniaki Saito, Hirotake Sano, Shinya Minatoguchi, Hisayoshi Fujiwara, Takahisa Hachiya, Akio Noma

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 μg/L and the detection limit was 0.01 μg/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sfas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sfas in 155 healthy subjects, ages 20-69. The mean value of sfas in men (2.50 ± 0.63 μg/L, n = 78) was significantly higher than that in women (2.01 ± 0.53 μg/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sfas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sfas tended to increase with aging, it remains to be clarified how Fas-mediated apoptosis relates to aging.

Original languageEnglish
Pages (from-to)1911-1914
Number of pages4
JournalClinical Chemistry
Volume42
Issue number12
Publication statusPublished - 21-12-1996
Externally publishedYes

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Aging of materials
Enzyme-Linked Immunosorbent Assay
Serum
Bilirubin
Hemoglobins
Apoptosis
Recovery
Limit of Detection
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Seishima, M., Takemura, M., Saito, K., Sano, H., Minatoguchi, S., Fujiwara, H., ... Noma, A. (1996). Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly. Clinical Chemistry, 42(12), 1911-1914.
Seishima, Mitsuru ; Takemura, Masao ; Saito, Kuniaki ; Sano, Hirotake ; Minatoguchi, Shinya ; Fujiwara, Hisayoshi ; Hachiya, Takahisa ; Noma, Akio. / Highly sensitive ELISA for soluble Fas in serum : Increased soluble Fas in the elderly. In: Clinical Chemistry. 1996 ; Vol. 42, No. 12. pp. 1911-1914.
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Seishima, M, Takemura, M, Saito, K, Sano, H, Minatoguchi, S, Fujiwara, H, Hachiya, T & Noma, A 1996, 'Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly', Clinical Chemistry, vol. 42, no. 12, pp. 1911-1914.

Highly sensitive ELISA for soluble Fas in serum : Increased soluble Fas in the elderly. / Seishima, Mitsuru; Takemura, Masao; Saito, Kuniaki; Sano, Hirotake; Minatoguchi, Shinya; Fujiwara, Hisayoshi; Hachiya, Takahisa; Noma, Akio.

In: Clinical Chemistry, Vol. 42, No. 12, 21.12.1996, p. 1911-1914.

Research output: Contribution to journalArticle

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AU - Seishima, Mitsuru

AU - Takemura, Masao

AU - Saito, Kuniaki

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AU - Hachiya, Takahisa

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Seishima M, Takemura M, Saito K, Sano H, Minatoguchi S, Fujiwara H et al. Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly. Clinical Chemistry. 1996 Dec 21;42(12):1911-1914.