Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly

Mitsuru Seishima; Masao Takemura; Kuniaki Saito; Hirotake Sano; Shinya Minatoguchi; Hisayoshi Fujiwara; Takahisa Hachiya; Akio Noma

Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly

Mitsuru Seishima, Masao Takemura, Kuniaki Saito, Hirotake Sano, Shinya Minatoguchi, Hisayoshi Fujiwara, Takahisa Hachiya, Akio Noma

Research output: Contribution to journal › Article

40 Citations (Scopus)

Abstract

We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 μg/L and the detection limit was 0.01 μg/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sfas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sfas in 155 healthy subjects, ages 20-69. The mean value of sfas in men (2.50 ± 0.63 μg/L, n = 78) was significantly higher than that in women (2.01 ± 0.53 μg/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sfas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sfas tended to increase with aging, it remains to be clarified how Fas-mediated apoptosis relates to aging.

Original language	English
Pages (from-to)	1911-1914
Number of pages	4
Journal	Clinical Chemistry
Volume	42
Issue number	12
Publication status	Published - 21-12-1996
Externally published	Yes

All Science Journal Classification (ASJC) codes

Clinical Biochemistry
Biochemistry, medical

Access to Document

Fingerprint Dive into the research topics of 'Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly'. Together they form a unique fingerprint.

Cite this

Seishima, M., Takemura, M., Saito, K., Sano, H., Minatoguchi, S., Fujiwara, H., Hachiya, T., & Noma, A. (1996). Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly. Clinical Chemistry, 42(12), 1911-1914.

@article{5995d2febece47afa7804cefee7c9215,

title = "Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly",

abstract = "We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 μg/L and the detection limit was 0.01 μg/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sfas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sfas in 155 healthy subjects, ages 20-69. The mean value of sfas in men (2.50 ± 0.63 μg/L, n = 78) was significantly higher than that in women (2.01 ± 0.53 μg/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sfas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sfas tended to increase with aging, it remains to be clarified how Fas-mediated apoptosis relates to aging.",

author = "Mitsuru Seishima and Masao Takemura and Kuniaki Saito and Hirotake Sano and Shinya Minatoguchi and Hisayoshi Fujiwara and Takahisa Hachiya and Akio Noma",

year = "1996",

month = dec,

day = "21",

language = "English",

volume = "42",

pages = "1911--1914",

journal = "Clinical Chemistry",

issn = "0009-9147",

publisher = "American Association for Clinical Chemistry Inc.",

number = "12",

}

TY - JOUR

T1 - Highly sensitive ELISA for soluble Fas in serum

T2 - Increased soluble Fas in the elderly

AU - Seishima, Mitsuru

AU - Takemura, Masao

AU - Saito, Kuniaki

AU - Sano, Hirotake

AU - Minatoguchi, Shinya

AU - Fujiwara, Hisayoshi

AU - Hachiya, Takahisa

AU - Noma, Akio

PY - 1996/12/21

Y1 - 1996/12/21

N2 - We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 μg/L and the detection limit was 0.01 μg/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sfas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sfas in 155 healthy subjects, ages 20-69. The mean value of sfas in men (2.50 ± 0.63 μg/L, n = 78) was significantly higher than that in women (2.01 ± 0.53 μg/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sfas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sfas tended to increase with aging, it remains to be clarified how Fas-mediated apoptosis relates to aging.

AB - We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 μg/L and the detection limit was 0.01 μg/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sfas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sfas in 155 healthy subjects, ages 20-69. The mean value of sfas in men (2.50 ± 0.63 μg/L, n = 78) was significantly higher than that in women (2.01 ± 0.53 μg/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sfas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sfas tended to increase with aging, it remains to be clarified how Fas-mediated apoptosis relates to aging.

UR - http://www.scopus.com/inward/record.url?scp=0029852934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029852934&partnerID=8YFLogxK

M3 - Article

C2 - 8969625

AN - SCOPUS:0029852934

VL - 42

SP - 1911

EP - 1914

JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

IS - 12

ER -