TY - JOUR
T1 - Highly sensitive ELISA for soluble Fas in serum
T2 - Increased soluble Fas in the elderly
AU - Seishima, Mitsuru
AU - Takemura, Masao
AU - Saito, Kuniaki
AU - Sano, Hirotake
AU - Minatoguchi, Shinya
AU - Fujiwara, Hisayoshi
AU - Hachiya, Takahisa
AU - Noma, Akio
PY - 1996
Y1 - 1996
N2 - We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 μg/L and the detection limit was 0.01 μg/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sfas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sfas in 155 healthy subjects, ages 20-69. The mean value of sfas in men (2.50 ± 0.63 μg/L, n = 78) was significantly higher than that in women (2.01 ± 0.53 μg/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sfas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sfas tended to increase with aging, it remains to be clarified how Fas-mediated apoptosis relates to aging.
AB - We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 μg/L and the detection limit was 0.01 μg/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sfas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sfas in 155 healthy subjects, ages 20-69. The mean value of sfas in men (2.50 ± 0.63 μg/L, n = 78) was significantly higher than that in women (2.01 ± 0.53 μg/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sfas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sfas tended to increase with aging, it remains to be clarified how Fas-mediated apoptosis relates to aging.
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U2 - 10.1093/clinchem/42.12.1911
DO - 10.1093/clinchem/42.12.1911
M3 - Article
C2 - 8969625
AN - SCOPUS:0029852934
VL - 42
SP - 1911
EP - 1914
JO - Clinical Chemistry
JF - Clinical Chemistry
SN - 0009-9147
IS - 12
ER -