Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly

Mitsuru Seishima, Masao Takemura, Kuniaki Saito, Hirotake Sano, Shinya Minatoguchi, Hisayoshi Fujiwara, Takahisa Hachiya, Akio Noma

Research output: Contribution to journalArticle

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Abstract

We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 μg/L and the detection limit was 0.01 μg/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sfas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sfas in 155 healthy subjects, ages 20-69. The mean value of sfas in men (2.50 ± 0.63 μg/L, n = 78) was significantly higher than that in women (2.01 ± 0.53 μg/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sfas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sfas tended to increase with aging, it remains to be clarified how Fas-mediated apoptosis relates to aging.

Original languageEnglish
Pages (from-to)1911-1914
Number of pages4
JournalClinical Chemistry
Volume42
Issue number12
Publication statusPublished - 21-12-1996
Externally publishedYes

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Aging of materials
Enzyme-Linked Immunosorbent Assay
Serum
Bilirubin
Hemoglobins
Apoptosis
Recovery
Limit of Detection
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Seishima, M., Takemura, M., Saito, K., Sano, H., Minatoguchi, S., Fujiwara, H., ... Noma, A. (1996). Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly. Clinical Chemistry, 42(12), 1911-1914.
Seishima, Mitsuru ; Takemura, Masao ; Saito, Kuniaki ; Sano, Hirotake ; Minatoguchi, Shinya ; Fujiwara, Hisayoshi ; Hachiya, Takahisa ; Noma, Akio. / Highly sensitive ELISA for soluble Fas in serum : Increased soluble Fas in the elderly. In: Clinical Chemistry. 1996 ; Vol. 42, No. 12. pp. 1911-1914.
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Seishima, M, Takemura, M, Saito, K, Sano, H, Minatoguchi, S, Fujiwara, H, Hachiya, T & Noma, A 1996, 'Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly', Clinical Chemistry, vol. 42, no. 12, pp. 1911-1914.

Highly sensitive ELISA for soluble Fas in serum : Increased soluble Fas in the elderly. / Seishima, Mitsuru; Takemura, Masao; Saito, Kuniaki; Sano, Hirotake; Minatoguchi, Shinya; Fujiwara, Hisayoshi; Hachiya, Takahisa; Noma, Akio.

In: Clinical Chemistry, Vol. 42, No. 12, 21.12.1996, p. 1911-1914.

Research output: Contribution to journalArticle

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T1 - Highly sensitive ELISA for soluble Fas in serum

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AU - Seishima, Mitsuru

AU - Takemura, Masao

AU - Saito, Kuniaki

AU - Sano, Hirotake

AU - Minatoguchi, Shinya

AU - Fujiwara, Hisayoshi

AU - Hachiya, Takahisa

AU - Noma, Akio

PY - 1996/12/21

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N2 - We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 μg/L and the detection limit was 0.01 μg/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sfas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sfas in 155 healthy subjects, ages 20-69. The mean value of sfas in men (2.50 ± 0.63 μg/L, n = 78) was significantly higher than that in women (2.01 ± 0.53 μg/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sfas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sfas tended to increase with aging, it remains to be clarified how Fas-mediated apoptosis relates to aging.

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Seishima M, Takemura M, Saito K, Sano H, Minatoguchi S, Fujiwara H et al. Highly sensitive ELISA for soluble Fas in serum: Increased soluble Fas in the elderly. Clinical Chemistry. 1996 Dec 21;42(12):1911-1914.