Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain

Hidekazu Tomimoto, Ichiro Akiguchi, Hideaki Wakita, Sinichi Nakamura, Jun Kimura

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We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction products were associated with membranous structure, likely the endoplasmic reticulum and cytoplasmic vesicles. These membranous localization of imunoreactivities at electron microscopic level corresponded to the perinuclear granular structures observed by light microscopic immunohistochemistry for endothelial nitric oxide synthase as well as NADPH diaphorase histochemistry.

Original languageEnglish
Pages (from-to)107-110
Number of pages4
JournalBrain Research
Issue number1
Publication statusPublished - 19-12-1994


All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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