Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain

Hidekazu Tomimoto, Ichiro Akiguchi, Hideaki Wakita, Sinichi Nakamura, Jun Kimura

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction products were associated with membranous structure, likely the endoplasmic reticulum and cytoplasmic vesicles. These membranous localization of imunoreactivities at electron microscopic level corresponded to the perinuclear granular structures observed by light microscopic immunohistochemistry for endothelial nitric oxide synthase as well as NADPH diaphorase histochemistry.

Original languageEnglish
Pages (from-to)107-110
Number of pages4
JournalBrain Research
Volume667
Issue number1
DOIs
Publication statusPublished - 19-12-1994

Fingerprint

Nitric Oxide Synthase Type III
Endothelial Cells
Cytoplasmic Vesicles
NADPH Dehydrogenase
Immunoelectron Microscopy
Brain
Endoplasmic Reticulum
Immunohistochemistry
Electrons
Light

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Tomimoto, Hidekazu ; Akiguchi, Ichiro ; Wakita, Hideaki ; Nakamura, Sinichi ; Kimura, Jun. / Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain. In: Brain Research. 1994 ; Vol. 667, No. 1. pp. 107-110.
@article{4c4433eead534f0ba4f52a432edd99bb,
title = "Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain",
abstract = "We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction products were associated with membranous structure, likely the endoplasmic reticulum and cytoplasmic vesicles. These membranous localization of imunoreactivities at electron microscopic level corresponded to the perinuclear granular structures observed by light microscopic immunohistochemistry for endothelial nitric oxide synthase as well as NADPH diaphorase histochemistry.",
author = "Hidekazu Tomimoto and Ichiro Akiguchi and Hideaki Wakita and Sinichi Nakamura and Jun Kimura",
year = "1994",
month = "12",
day = "19",
doi = "10.1016/0006-8993(94)91718-3",
language = "English",
volume = "667",
pages = "107--110",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain. / Tomimoto, Hidekazu; Akiguchi, Ichiro; Wakita, Hideaki; Nakamura, Sinichi; Kimura, Jun.

In: Brain Research, Vol. 667, No. 1, 19.12.1994, p. 107-110.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain

AU - Tomimoto, Hidekazu

AU - Akiguchi, Ichiro

AU - Wakita, Hideaki

AU - Nakamura, Sinichi

AU - Kimura, Jun

PY - 1994/12/19

Y1 - 1994/12/19

N2 - We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction products were associated with membranous structure, likely the endoplasmic reticulum and cytoplasmic vesicles. These membranous localization of imunoreactivities at electron microscopic level corresponded to the perinuclear granular structures observed by light microscopic immunohistochemistry for endothelial nitric oxide synthase as well as NADPH diaphorase histochemistry.

AB - We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction products were associated with membranous structure, likely the endoplasmic reticulum and cytoplasmic vesicles. These membranous localization of imunoreactivities at electron microscopic level corresponded to the perinuclear granular structures observed by light microscopic immunohistochemistry for endothelial nitric oxide synthase as well as NADPH diaphorase histochemistry.

UR - http://www.scopus.com/inward/record.url?scp=0028575341&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028575341&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(94)91718-3

DO - 10.1016/0006-8993(94)91718-3

M3 - Article

VL - 667

SP - 107

EP - 110

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -