Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain

Hidekazu Tomimoto; Ichiro Akiguchi; Hideaki Wakita; Sinichi Nakamura; Jun Kimura

doi:10.1016/0006-8993(94)91718-3

Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain

Hidekazu Tomimoto, Ichiro Akiguchi, Hideaki Wakita, Sinichi Nakamura, Jun Kimura

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction products were associated with membranous structure, likely the endoplasmic reticulum and cytoplasmic vesicles. These membranous localization of imunoreactivities at electron microscopic level corresponded to the perinuclear granular structures observed by light microscopic immunohistochemistry for endothelial nitric oxide synthase as well as NADPH diaphorase histochemistry.

Original language	English
Pages (from-to)	107-110
Number of pages	4
Journal	Brain Research
Volume	667
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(94)91718-3
Publication status	Published - 19-12-1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(94)91718-3

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title = "Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain",

abstract = "We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction products were associated with membranous structure, likely the endoplasmic reticulum and cytoplasmic vesicles. These membranous localization of imunoreactivities at electron microscopic level corresponded to the perinuclear granular structures observed by light microscopic immunohistochemistry for endothelial nitric oxide synthase as well as NADPH diaphorase histochemistry.",

author = "Hidekazu Tomimoto and Ichiro Akiguchi and Hideaki Wakita and Sinichi Nakamura and Jun Kimura",

note = "Funding Information: Sclerosis and Peripheral Neuropathy from the Japanese Ministry of Health and Welfare and a grant from Kanae Foundation of Research for New Medicine (to H.T.). Funding Information: This work was supported by a Grand-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Grants-in-Aid for Amyotrophic Lateral",

year = "1994",

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doi = "10.1016/0006-8993(94)91718-3",

language = "English",

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T1 - Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain

AU - Tomimoto, Hidekazu

AU - Akiguchi, Ichiro

AU - Wakita, Hideaki

AU - Nakamura, Sinichi

AU - Kimura, Jun

N1 - Funding Information: Sclerosis and Peripheral Neuropathy from the Japanese Ministry of Health and Welfare and a grant from Kanae Foundation of Research for New Medicine (to H.T.). Funding Information: This work was supported by a Grand-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Grants-in-Aid for Amyotrophic Lateral

PY - 1994/12/19

Y1 - 1994/12/19

N2 - We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction products were associated with membranous structure, likely the endoplasmic reticulum and cytoplasmic vesicles. These membranous localization of imunoreactivities at electron microscopic level corresponded to the perinuclear granular structures observed by light microscopic immunohistochemistry for endothelial nitric oxide synthase as well as NADPH diaphorase histochemistry.

AB - We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction products were associated with membranous structure, likely the endoplasmic reticulum and cytoplasmic vesicles. These membranous localization of imunoreactivities at electron microscopic level corresponded to the perinuclear granular structures observed by light microscopic immunohistochemistry for endothelial nitric oxide synthase as well as NADPH diaphorase histochemistry.

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Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain

Abstract

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