Histologic study of collagen and stem cells after radiofrequency treatment for aging skin

Yusuke Yokoyama, Hirotaka Akita, Seiji Hasegawa, Kei Negishi, Hirohiko Akamatsu, Kayoko Matsunaga

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Background Monopolar radiofrequency (mRF) devices have been shown to be clinically effective for treating aging skin, but there are few histologic studies about the mechanisms. Objective To histologically analyze chronologic and quantitative change in collagens after mRF treatment to determine the mechanisms of the antiaging effect. Methods Five patients were enrolled in this study. Skin specimens were taken before and 1 and 3 months after treatment. Immunostaining was performed to determine change in type I and III collagen levels and stem and other cell counts in skin layers. Results In all cases, both types of collagen significantly increased after irradiation in the dermis (p <.05), and their changes were noticed uniformly in all layers. No significant change was noticed in stem and other cell counts. Conclusions This study histologically demonstrated that type I and III collagen increased significantly in the dermis after mRF treatment. The amount of stem cells did not affect the increase in collagens.

Original languageEnglish
Pages (from-to)390-397
Number of pages8
JournalDermatologic Surgery
Issue number4
Publication statusPublished - 04-2014

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology


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