Histone deacetylase inhibitor attenuates neurotoxicity of clioquinol in PC12 cells

Takao Fukui, Kunihiko Asakura, Chika Hikichi, Tomomasa Ishikawa, Rie Murai, Seiko Hirota, Ken ichiro Murate, Madoko Kizawa, Akihiro Ueda, Shinji Ito, Tatsuro Mutoh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Clioquinol is considered to be a causative agent of subacute myelo-optico neuropathy (SMON), although the pathogenesis of SMON is yet to be elucidated. We have previously shown that clioquinol inhibits nerve growth factor (NGF)-induced Trk autophosphorylation in PC12 cells transformed with human Trk cDNA. To explore the further mechanism of neuronal damage by clioquinol, we evaluated the acetylation status of histones in PC12 cells. Clioquinol reduced the level of histone acetylation, and the histone deacetylase (HDAC) inhibitor Trichostatin A upregulated acetylated histones and prevented the neuronal cell damage caused by clioquinol. In addition, treatment with HDAC inhibitor decreased neurite retraction and restored the inhibition of NGF-induced Trk autophosphorylation by clioquinol. Thus, clioquinol induced neuronal cell death via deacetylation of histones, and HDAC inhibitor alleviates the neurotoxicity of clioquinol. Clioquinol is now used as a potential medicine for malignancies and neurodegenerative diseases. Therefore, HDAC inhibitors can be used as a candidate medicine for the prevention of its side effects on neuronal cells.

Original languageEnglish
Pages (from-to)112-118
Number of pages7
JournalToxicology
Volume331
DOIs
Publication statusPublished - 04-05-2015

Fingerprint

Clioquinol
Histone Deacetylase Inhibitors
PC12 Cells
Histones
Acetylation
Nerve Growth Factor
Medicine
trichostatin A
Neurodegenerative diseases
Bioelectric potentials
Neurites
Cell death
Neurodegenerative Diseases
Cell Death
Complementary DNA
Cells

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Medicine(all)

Cite this

Fukui, T., Asakura, K., Hikichi, C., Ishikawa, T., Murai, R., Hirota, S., ... Mutoh, T. (2015). Histone deacetylase inhibitor attenuates neurotoxicity of clioquinol in PC12 cells. Toxicology, 331, 112-118. https://doi.org/10.1016/j.tox.2015.01.013
Fukui, Takao ; Asakura, Kunihiko ; Hikichi, Chika ; Ishikawa, Tomomasa ; Murai, Rie ; Hirota, Seiko ; Murate, Ken ichiro ; Kizawa, Madoko ; Ueda, Akihiro ; Ito, Shinji ; Mutoh, Tatsuro. / Histone deacetylase inhibitor attenuates neurotoxicity of clioquinol in PC12 cells. In: Toxicology. 2015 ; Vol. 331. pp. 112-118.
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Fukui, T, Asakura, K, Hikichi, C, Ishikawa, T, Murai, R, Hirota, S, Murate, KI, Kizawa, M, Ueda, A, Ito, S & Mutoh, T 2015, 'Histone deacetylase inhibitor attenuates neurotoxicity of clioquinol in PC12 cells', Toxicology, vol. 331, pp. 112-118. https://doi.org/10.1016/j.tox.2015.01.013

Histone deacetylase inhibitor attenuates neurotoxicity of clioquinol in PC12 cells. / Fukui, Takao; Asakura, Kunihiko; Hikichi, Chika; Ishikawa, Tomomasa; Murai, Rie; Hirota, Seiko; Murate, Ken ichiro; Kizawa, Madoko; Ueda, Akihiro; Ito, Shinji; Mutoh, Tatsuro.

In: Toxicology, Vol. 331, 04.05.2015, p. 112-118.

Research output: Contribution to journalArticle

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AU - Fukui, Takao

AU - Asakura, Kunihiko

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AU - Ishikawa, Tomomasa

AU - Murai, Rie

AU - Hirota, Seiko

AU - Murate, Ken ichiro

AU - Kizawa, Madoko

AU - Ueda, Akihiro

AU - Ito, Shinji

AU - Mutoh, Tatsuro

PY - 2015/5/4

Y1 - 2015/5/4

N2 - Clioquinol is considered to be a causative agent of subacute myelo-optico neuropathy (SMON), although the pathogenesis of SMON is yet to be elucidated. We have previously shown that clioquinol inhibits nerve growth factor (NGF)-induced Trk autophosphorylation in PC12 cells transformed with human Trk cDNA. To explore the further mechanism of neuronal damage by clioquinol, we evaluated the acetylation status of histones in PC12 cells. Clioquinol reduced the level of histone acetylation, and the histone deacetylase (HDAC) inhibitor Trichostatin A upregulated acetylated histones and prevented the neuronal cell damage caused by clioquinol. In addition, treatment with HDAC inhibitor decreased neurite retraction and restored the inhibition of NGF-induced Trk autophosphorylation by clioquinol. Thus, clioquinol induced neuronal cell death via deacetylation of histones, and HDAC inhibitor alleviates the neurotoxicity of clioquinol. Clioquinol is now used as a potential medicine for malignancies and neurodegenerative diseases. Therefore, HDAC inhibitors can be used as a candidate medicine for the prevention of its side effects on neuronal cells.

AB - Clioquinol is considered to be a causative agent of subacute myelo-optico neuropathy (SMON), although the pathogenesis of SMON is yet to be elucidated. We have previously shown that clioquinol inhibits nerve growth factor (NGF)-induced Trk autophosphorylation in PC12 cells transformed with human Trk cDNA. To explore the further mechanism of neuronal damage by clioquinol, we evaluated the acetylation status of histones in PC12 cells. Clioquinol reduced the level of histone acetylation, and the histone deacetylase (HDAC) inhibitor Trichostatin A upregulated acetylated histones and prevented the neuronal cell damage caused by clioquinol. In addition, treatment with HDAC inhibitor decreased neurite retraction and restored the inhibition of NGF-induced Trk autophosphorylation by clioquinol. Thus, clioquinol induced neuronal cell death via deacetylation of histones, and HDAC inhibitor alleviates the neurotoxicity of clioquinol. Clioquinol is now used as a potential medicine for malignancies and neurodegenerative diseases. Therefore, HDAC inhibitors can be used as a candidate medicine for the prevention of its side effects on neuronal cells.

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Fukui T, Asakura K, Hikichi C, Ishikawa T, Murai R, Hirota S et al. Histone deacetylase inhibitor attenuates neurotoxicity of clioquinol in PC12 cells. Toxicology. 2015 May 4;331:112-118. https://doi.org/10.1016/j.tox.2015.01.013