TY - JOUR
T1 - Histopathological assessment of localized proliferation in cases of Bowen's disease using immunostaining and a laser cytometer
AU - Murakami, Masamoto
AU - Hashimoto, Yoshiko
AU - Tsukinoki, Keiichi
AU - Matsunaga, Kayoko
AU - Mizoguchi, Yoshikazu
AU - Horibe, Yoshimune
AU - Tashiro, Kazuhiro
AU - Watanabe, Yoshihisa
AU - Kasahara, Masao
N1 - Funding Information:
Acknowledgements The authors thank Mr. Yahachi Yagi (Clinical Laboratory, Shizuoka, Japan), Dr. Yutaka Hirasawa and Ms. Yuko Ito (Clinical Laboratory, Fujita Health University Hospital, Aichi, Japan) for their expert technical assistance. The authors also acknowledge the kind linguistic help of Dr. Malcolm Moore (National Cancer Center, Tokyo, Japan). This investigation was supported by a grant from the Fujita Health University, School of Medicine.
PY - 1998
Y1 - 1998
N2 - In order to evaluate the localized proliferative activity of intratumor cells in Bowen's disease using tissue sections, skin specimens from ten patients were compared with skin samples from seven normal individuals for their expression of proliferating cell nuclear antigen (PCNA), Ki-67 immunostaining and intranuclear DNA contents, quantitated with a laser cytometer (LCM). In normal epidermis, the largest proportion of PCNA- and Ki-67-positive cells was observed in the basal cell layer, with the amounts decreasing through the suprabasal cell layer towards the prickle cell layer. Examination by LCM also revealed the highest average fluorescence intensity of individual nuclei in the basal cell layer and, as with the immunohistological parameters, reducing towards the upper layer of the epidermis. In the Bowen's disease tissue sections, the largest proportion of PCNA- and Ki-67-positive cells was found in contact with the basement membrane (base of the tumor), with lower amounts in the center of the tumor nest and in the marginal epidermis. The average fluorescence intensities of individual nuclei were in line with these results. These results show that tumor cells distributed in Bowen's disease tumor nests have different proliferative activities depending on their location.
AB - In order to evaluate the localized proliferative activity of intratumor cells in Bowen's disease using tissue sections, skin specimens from ten patients were compared with skin samples from seven normal individuals for their expression of proliferating cell nuclear antigen (PCNA), Ki-67 immunostaining and intranuclear DNA contents, quantitated with a laser cytometer (LCM). In normal epidermis, the largest proportion of PCNA- and Ki-67-positive cells was observed in the basal cell layer, with the amounts decreasing through the suprabasal cell layer towards the prickle cell layer. Examination by LCM also revealed the highest average fluorescence intensity of individual nuclei in the basal cell layer and, as with the immunohistological parameters, reducing towards the upper layer of the epidermis. In the Bowen's disease tissue sections, the largest proportion of PCNA- and Ki-67-positive cells was found in contact with the basement membrane (base of the tumor), with lower amounts in the center of the tumor nest and in the marginal epidermis. The average fluorescence intensities of individual nuclei were in line with these results. These results show that tumor cells distributed in Bowen's disease tumor nests have different proliferative activities depending on their location.
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U2 - 10.1007/s004030050332
DO - 10.1007/s004030050332
M3 - Article
C2 - 9763306
AN - SCOPUS:0031659167
SN - 0340-3696
VL - 290
SP - 435
EP - 440
JO - Archives of Dermatological Research
JF - Archives of Dermatological Research
IS - 8
ER -