Homologous recombinational repair factors are recruited and loaded onto the viral DNA genome in Epstein-Barr virus replication compartments

Ayumi Kudoh, Satoko Iwahori, Yoshitaka Sato, Sanae Nakayama, Hiroki Isomura, Takayuki Murata, Tatsuya Tsurumi

Research output: Contribution to journalArticle

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Abstract

Homologous recombination is an important biological process that facilitates genome rearrangement and repair of DNA double-strand breaks (DSBs). The induction of Epstein-Barr virus (EBV) lytic replication induces ataxia telangiectasia-mutated (ATM)-dependent DNA damage checkpoint signaling, leading to the clustering of phosphorylated ATM and Mre11/Rad50/Nbs1 (MRN) complexes to sites of viral genome synthesis in nuclei. Here we report that homologous recombinational repair (HRR) factors such as replication protein A (RPA), Rad51, and Rad52 as well as MRN complexes are recruited and loaded onto the newly synthesized viral genome in replication compartments. The 32-kDa subunit of RPA is extensively phosphorylated at sites in accordance with those with ATM. The hyperphosphorylation of RPA32 causes a change in RPA conformation, resulting in a switch from the catalysis of DNA replication to the participation in DNA repair. The levels of Rad51 and phosphorylated RPA were found to increase with the progression of viral productive replication, while that of Rad52 proved constant. Furthermore, biochemical fractionation revealed increases in levels of DNA-bound forms of these HRRs. Bromodeoxyuridine-labeled chromatin immunoprecipitation and PCR analyses confirmed the loading of RPA, Rad 51, Rad52, and Mre11 onto newly synthesized viral DNA, and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling analysis demonstrated DSBs in the EBV replication compartments. HRR factors might be recruited to repair DSBs on the viral genome in viral replication compartments. RNA interference knockdown of RPA32 and Rad51 prevented viral DNA synthesis remarkably, suggesting that homologous recombination and/or repair of viral DNA genome might occur, coupled with DNA replication to facilitate viral genome synthesis.

Original languageEnglish
Pages (from-to)6641-6651
Number of pages11
JournalJournal of Virology
Volume83
Issue number13
DOIs
Publication statusPublished - 01-07-2009
Externally publishedYes

Fingerprint

Replication Protein A
Recombinational DNA Repair
Human herpesvirus 4
Viral Genome
Viral DNA
Virus Replication
virus replication
Human Herpesvirus 4
Ataxia Telangiectasia
genome
DNA
DNA Replication
homologous recombination
DNA replication
DNA repair
proteins
synthesis
Biological Phenomena
Protein Conformation
Double-Stranded DNA Breaks

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Kudoh, Ayumi ; Iwahori, Satoko ; Sato, Yoshitaka ; Nakayama, Sanae ; Isomura, Hiroki ; Murata, Takayuki ; Tsurumi, Tatsuya. / Homologous recombinational repair factors are recruited and loaded onto the viral DNA genome in Epstein-Barr virus replication compartments. In: Journal of Virology. 2009 ; Vol. 83, No. 13. pp. 6641-6651.
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Homologous recombinational repair factors are recruited and loaded onto the viral DNA genome in Epstein-Barr virus replication compartments. / Kudoh, Ayumi; Iwahori, Satoko; Sato, Yoshitaka; Nakayama, Sanae; Isomura, Hiroki; Murata, Takayuki; Tsurumi, Tatsuya.

In: Journal of Virology, Vol. 83, No. 13, 01.07.2009, p. 6641-6651.

Research output: Contribution to journalArticle

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