HSP47: A tissue-specific, transformation-sensitive, collagen-binding heat shock protein of chicken embryo fibroblasts

Kazunori Hirayoshi, Hitoshi Kudo, Hajime Takechi, Akira Nakai, Akihiro Iwamatsu, Kenneth M. Yamada, Kazuhiro Nagata

Research output: Contribution to journalArticle

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Abstract

We report the isolation and characterization of a cDNA clone encoding HSP47, a transformation-sensitive heat shock protein that binds to collagen. A cDNA library was prepared from total RNA isolated from heat-shocked chicken embryo fibroblasts and screened by using oligonucleotide mixtures prepared on the basis of the N-terminal amino acid sequence of biochemically purified HSP47. The cDNA insert contained 3,278 bp, which encoded a 15-amino-acid signal peptide and a mature protein coding region consisting of 390 amino acid residues; it also included part of the 5′ noncoding region and a long 3′ noncoding region. The deduced amino acid sequence revealed an RDEL sequence at the C terminus, which is a variant of the KDEL retention signal for retention of proteins in the endoplasmic reticulum. Northern (RNA) blot analyses and nuclear run-on assays established that the induction of HSP47 by heat shock and its suppression after transformation of chicken embryo fibroblasts by Rous sarcoma virus are regulated at the transcriptional level. A homology search revealed that this protein belongs to the serpin family, the superfamily of plasma serine protease inhibitors. Although structurally homologous to the serpins, HSP47 lacks the active site thought to be essential for the inhibition of proteases and does not appear to bind to intracellular proteases. HSP47 is the first heat shock protein found to be a member of the serpin superfamily. Conversely, it is the first serpin family member that is not secreted from cells, which could be explained by acquisition of the RDEL retention signal during evolution.

Original languageEnglish
Pages (from-to)4036-4044
Number of pages9
JournalMolecular and Cellular Biology
Volume11
Issue number8
DOIs
Publication statusPublished - 01-01-1991

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Serpins
Heat-Shock Proteins
Chickens
Carrier Proteins
Collagen
Embryonic Structures
Fibroblasts
Amino Acid Sequence
Complementary DNA
Hot Temperature
RNA
Amino Acids
Rous sarcoma virus
Serine Proteinase Inhibitors
Protein Sorting Signals
Gene Library
Oligonucleotides
Endoplasmic Reticulum
Northern Blotting
Open Reading Frames

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Hirayoshi, Kazunori ; Kudo, Hitoshi ; Takechi, Hajime ; Nakai, Akira ; Iwamatsu, Akihiro ; Yamada, Kenneth M. ; Nagata, Kazuhiro. / HSP47 : A tissue-specific, transformation-sensitive, collagen-binding heat shock protein of chicken embryo fibroblasts. In: Molecular and Cellular Biology. 1991 ; Vol. 11, No. 8. pp. 4036-4044.
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HSP47 : A tissue-specific, transformation-sensitive, collagen-binding heat shock protein of chicken embryo fibroblasts. / Hirayoshi, Kazunori; Kudo, Hitoshi; Takechi, Hajime; Nakai, Akira; Iwamatsu, Akihiro; Yamada, Kenneth M.; Nagata, Kazuhiro.

In: Molecular and Cellular Biology, Vol. 11, No. 8, 01.01.1991, p. 4036-4044.

Research output: Contribution to journalArticle

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