Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

Hiroaki Sugiyama, Kazuhisa Maeda, Masayuki Yamato, Ryuhei Hayashi, Takeshi Soma, Yasutaka Hayashida, Joseph Yang, Masayuki Shirakabe, Akifumi Matsuyama, Akihiko Kikuchi, Yoshiki Sawa, Teruo Okano, Yasuo Tano, Kohji Nishida

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22 Citations (Scopus)

Abstract

We examined a novel human feeder cell layer of mesenchymal stem cells harvested from human adipose tissues. Gene expression analyses and colony-forming assay with human primary epithelial cells showed that the adipose tissue-derived mesenchymal stem cells produced various factors to support epithelial stem/progenitor maintenance and cell growth. Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces.

Original languageEnglish
Pages (from-to)445-449
Number of pages5
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume2
Issue number7
DOIs
Publication statusPublished - 10-2008

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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    Sugiyama, H., Maeda, K., Yamato, M., Hayashi, R., Soma, T., Hayashida, Y., Yang, J., Shirakabe, M., Matsuyama, A., Kikuchi, A., Sawa, Y., Okano, T., Tano, Y., & Nishida, K. (2008). Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells. Journal of Tissue Engineering and Regenerative Medicine, 2(7), 445-449. https://doi.org/10.1002/term.111