Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

Hiroaki Sugiyama, Kazuhisa Maeda, Masayuki Yamato, Ryuhei Hayashi, Takeshi Soma, Yasutaka Hayashida, Joseph Yang, Masayuki Shirakabe, Akifumi Matsuyama, Akihiko Kikuchi, Yoshiki Sawa, Teruo Okano, Yasuo Tano, Kohji Nishida

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We examined a novel human feeder cell layer of mesenchymal stem cells harvested from human adipose tissues. Gene expression analyses and colony-forming assay with human primary epithelial cells showed that the adipose tissue-derived mesenchymal stem cells produced various factors to support epithelial stem/progenitor maintenance and cell growth. Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces.

Original languageEnglish
Pages (from-to)445-449
Number of pages5
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume2
Issue number7
DOIs
Publication statusPublished - 01-10-2008

Fingerprint

Feeder Cells
Stem cells
Mesenchymal Stromal Cells
Adipose Tissue
Epithelial Cells
Tissue
Cell growth
Cell culture
Gene expression
Assays
Stem Cells
Cell Culture Techniques
Cells
Maintenance
Gene Expression
Temperature
Growth

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Sugiyama, Hiroaki ; Maeda, Kazuhisa ; Yamato, Masayuki ; Hayashi, Ryuhei ; Soma, Takeshi ; Hayashida, Yasutaka ; Yang, Joseph ; Shirakabe, Masayuki ; Matsuyama, Akifumi ; Kikuchi, Akihiko ; Sawa, Yoshiki ; Okano, Teruo ; Tano, Yasuo ; Nishida, Kohji. / Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells. In: Journal of Tissue Engineering and Regenerative Medicine. 2008 ; Vol. 2, No. 7. pp. 445-449.
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Sugiyama, H, Maeda, K, Yamato, M, Hayashi, R, Soma, T, Hayashida, Y, Yang, J, Shirakabe, M, Matsuyama, A, Kikuchi, A, Sawa, Y, Okano, T, Tano, Y & Nishida, K 2008, 'Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells', Journal of Tissue Engineering and Regenerative Medicine, vol. 2, no. 7, pp. 445-449. https://doi.org/10.1002/term.111

Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells. / Sugiyama, Hiroaki; Maeda, Kazuhisa; Yamato, Masayuki; Hayashi, Ryuhei; Soma, Takeshi; Hayashida, Yasutaka; Yang, Joseph; Shirakabe, Masayuki; Matsuyama, Akifumi; Kikuchi, Akihiko; Sawa, Yoshiki; Okano, Teruo; Tano, Yasuo; Nishida, Kohji.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 2, No. 7, 01.10.2008, p. 445-449.

Research output: Contribution to journalArticle

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AU - Sugiyama, Hiroaki

AU - Maeda, Kazuhisa

AU - Yamato, Masayuki

AU - Hayashi, Ryuhei

AU - Soma, Takeshi

AU - Hayashida, Yasutaka

AU - Yang, Joseph

AU - Shirakabe, Masayuki

AU - Matsuyama, Akifumi

AU - Kikuchi, Akihiko

AU - Sawa, Yoshiki

AU - Okano, Teruo

AU - Tano, Yasuo

AU - Nishida, Kohji

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