Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

Hiroaki Sugiyama; Kazuhisa Maeda; Masayuki Yamato; Ryuhei Hayashi; Takeshi Soma; Yasutaka Hayashida; Joseph Yang; Masayuki Shirakabe; Akifumi Matsuyama; Akihiko Kikuchi; Yoshiki Sawa; Teruo Okano; Yasuo Tano; Kohji Nishida

doi:10.1002/term.111

Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

Hiroaki Sugiyama, Kazuhisa Maeda, Masayuki Yamato, Ryuhei Hayashi, Takeshi Soma, Yasutaka Hayashida, Joseph Yang, Masayuki Shirakabe, Akifumi Matsuyama, Akihiko Kikuchi, Yoshiki Sawa, Teruo Okano, Yasuo Tano, Kohji Nishida

Regenerative Medicine

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

We examined a novel human feeder cell layer of mesenchymal stem cells harvested from human adipose tissues. Gene expression analyses and colony-forming assay with human primary epithelial cells showed that the adipose tissue-derived mesenchymal stem cells produced various factors to support epithelial stem/progenitor maintenance and cell growth. Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces.

Original language	English
Pages (from-to)	445-449
Number of pages	5
Journal	Journal of Tissue Engineering and Regenerative Medicine
Volume	2
Issue number	7
DOIs	https://doi.org/10.1002/term.111
Publication status	Published - 10-2008

All Science Journal Classification (ASJC) codes

Medicine (miscellaneous)
Biomaterials
Biomedical Engineering

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Sugiyama, H., Maeda, K., Yamato, M., Hayashi, R., Soma, T., Hayashida, Y., Yang, J., Shirakabe, M., Matsuyama, A., Kikuchi, A., Sawa, Y., Okano, T., Tano, Y., & Nishida, K. (2008). Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells. Journal of Tissue Engineering and Regenerative Medicine, 2(7), 445-449. https://doi.org/10.1002/term.111

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abstract = "We examined a novel human feeder cell layer of mesenchymal stem cells harvested from human adipose tissues. Gene expression analyses and colony-forming assay with human primary epithelial cells showed that the adipose tissue-derived mesenchymal stem cells produced various factors to support epithelial stem/progenitor maintenance and cell growth. Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces.",

author = "Hiroaki Sugiyama and Kazuhisa Maeda and Masayuki Yamato and Ryuhei Hayashi and Takeshi Soma and Yasutaka Hayashida and Joseph Yang and Masayuki Shirakabe and Akifumi Matsuyama and Akihiko Kikuchi and Yoshiki Sawa and Teruo Okano and Yasuo Tano and Kohji Nishida",

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Sugiyama, H, Maeda, K, Yamato, M, Hayashi, R, Soma, T, Hayashida, Y, Yang, J, Shirakabe, M, Matsuyama, A, Kikuchi, A, Sawa, Y, Okano, T, Tano, Y & Nishida, K 2008, 'Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells', Journal of Tissue Engineering and Regenerative Medicine, vol. 2, no. 7, pp. 445-449. https://doi.org/10.1002/term.111

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T1 - Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

AU - Sugiyama, Hiroaki

AU - Maeda, Kazuhisa

AU - Yamato, Masayuki

AU - Hayashi, Ryuhei

AU - Soma, Takeshi

AU - Hayashida, Yasutaka

AU - Yang, Joseph

AU - Shirakabe, Masayuki

AU - Matsuyama, Akifumi

AU - Kikuchi, Akihiko

AU - Sawa, Yoshiki

AU - Okano, Teruo

AU - Tano, Yasuo

AU - Nishida, Kohji

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Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells

Abstract

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