Human B-lymphocytes express α2-6-sialylated 6-sulfo-N- acetyllactosamine serving as a preferred ligand for CD22/siglec-2

Naoko Kimura, Katsuyuki Ohmori, Keiko Miyazaki, Mineko Izawa, Yuji Matsuzaki, Yosuke Yasuda, Hiromu Takematsu, Yasunori Kozutsumi, Akihiko Moriyama, Reiji Kannagi

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

CD22/Siglec-2, an important inhibitory co-receptor on B-lymphocytes, is known to recognize α2-6-sialylated glycan as a specific ligand. Here we propose that the α2-6-sialylated and 6-GlcNAc-sulfated determinant serves as a preferred ligand for CD22 because the binding of a human B-cell line to CD22 was almost completely abrogated after incubating the cells with NaClO 3, an inhibitor of cellular sulfate metabolism, and was also significantly inhibited by a newly generated monoclonal antibody specific to the α2-6-sialylated 6-sulfo-N-acetyllactosamine (LacNAc) determinant (KN343, murine IgM). The α2-6-sialylated 6-sulfo-LacNAc determinant defined by the antibody was significantly expressed on a majority of normal human peripheral B-lymphocytes as well as follicular B-lymphocytes in peripheral lymph nodes. The determinant was also expressed in endothelial cells of high endothelial venules of secondary lymphoid tissues, including lymph nodes, tonsils, and intestine-associated lymphoid tissues, more strongly than on B-lymphocytes, suggesting a role for CD22 in B-cell interaction with blood vessels and trafficking. These results indicate that the α2-6-sialylated 6-sulfo-LacNAc determinant serves as an endogenous ligand for human CD22 and suggest the possibility that 6-GlcNAc sulfation as well as α2-6- sialylation may regulate CD22/Siglec-2 functions in humans.

Original languageEnglish
Pages (from-to)32200-32207
Number of pages8
JournalJournal of Biological Chemistry
Volume282
Issue number44
DOIs
Publication statusPublished - 02-11-2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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