Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae

Ryuji Nomura, Asuka Kiyota, Etsuko Suzaki, Katsuko Kataoka, Yoshihide Ohe, Kaoru Miyamoto, Takao Senda, Toyoshi Fujimoto

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Abstract

CD13, a receptor for human coronavirus 229E (HCoV-229E), was identified as a major component of the Triton X-100-resistant membrane microdomain in human fibroblasts. The incubation of living fibroblasts with an anti-CD13 antibody on ice gave punctate labeling that was evenly distributed on the cell surface, but raising the temperature to 37°C before fixation caused aggregation of the labeling. The aggregated labeling of CD13 colocalized with caveolin-1 in most cells. The HCoV-229E virus particle showed a binding and redistribution pattern that was similar to that caused by the anti-CD13 antibody: the virus bound to the cell evenly when incubated on ice but became colocalized with caveolin-1 at 37°C; importantly, the virus also caused sequestration of CD13 to the caveolin-1-positive area. Electron microscopy confirmed that HCoV-229E was localized near or at the orifice of caveolae after incubation at 37°C. The depletion of plasmalemmal cholesterol with methyl β-cyclodextrin significantly reduced the HCoV-229E redistribution and subsequent infection. A caveolin-1 knockdown by RNA interference also reduced the HCoV-229E infection considerably. The results indicate that HCoV-229E first binds to CD13 in the Triton X-100-resistant microdomain, then clusters CD13 by cross-linking, and thereby reaches the caveolar region before entering cells.

Original languageEnglish
Pages (from-to)8701-8708
Number of pages8
JournalJournal of Virology
Volume78
Issue number16
DOIs
Publication statusPublished - 08-2004

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Nomura, R., Kiyota, A., Suzaki, E., Kataoka, K., Ohe, Y., Miyamoto, K., Senda, T., & Fujimoto, T. (2004). Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae. Journal of Virology, 78(16), 8701-8708. https://doi.org/10.1128/JVI.78.16.8701-8708.2004