Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae

Ryuji Nomura, Asuka Kiyota, Etsuko Suzaki, Katsuko Kataoka, Yoshihide Ohe, Kaoru Miyamoto, Takao Senda, Toyoshi Fujimoto

Research output: Contribution to journalArticle

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Abstract

CD13, a receptor for human coronavirus 229E (HCoV-229E), was identified as a major component of the Triton X-100-resistant membrane microdomain in human fibroblasts. The incubation of living fibroblasts with an anti-CD13 antibody on ice gave punctate labeling that was evenly distributed on the cell surface, but raising the temperature to 37°C before fixation caused aggregation of the labeling. The aggregated labeling of CD13 colocalized with caveolin-1 in most cells. The HCoV-229E virus particle showed a binding and redistribution pattern that was similar to that caused by the anti-CD13 antibody: the virus bound to the cell evenly when incubated on ice but became colocalized with caveolin-1 at 37°C; importantly, the virus also caused sequestration of CD13 to the caveolin-1-positive area. Electron microscopy confirmed that HCoV-229E was localized near or at the orifice of caveolae after incubation at 37°C. The depletion of plasmalemmal cholesterol with methyl β-cyclodextrin significantly reduced the HCoV-229E redistribution and subsequent infection. A caveolin-1 knockdown by RNA interference also reduced the HCoV-229E infection considerably. The results indicate that HCoV-229E first binds to CD13 in the Triton X-100-resistant microdomain, then clusters CD13 by cross-linking, and thereby reaches the caveolar region before entering cells.

Original languageEnglish
Pages (from-to)8701-8708
Number of pages8
JournalJournal of Virology
Volume78
Issue number16
DOIs
Publication statusPublished - 01-08-2004

Fingerprint

Human coronavirus 229E
Human Coronavirus 229E
Caveolae
Caveolin 1
Octoxynol
Ice
cells
fibroblasts
Anti-Idiotypic Antibodies
ice
Fibroblasts
Coronavirus Infections
Membrane Microdomains
Viruses
viruses
antibodies
cyclodextrins
Cyclodextrins
RNA Interference
RNA interference

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Nomura, R., Kiyota, A., Suzaki, E., Kataoka, K., Ohe, Y., Miyamoto, K., ... Fujimoto, T. (2004). Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae. Journal of Virology, 78(16), 8701-8708. https://doi.org/10.1128/JVI.78.16.8701-8708.2004
Nomura, Ryuji ; Kiyota, Asuka ; Suzaki, Etsuko ; Kataoka, Katsuko ; Ohe, Yoshihide ; Miyamoto, Kaoru ; Senda, Takao ; Fujimoto, Toyoshi. / Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae. In: Journal of Virology. 2004 ; Vol. 78, No. 16. pp. 8701-8708.
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abstract = "CD13, a receptor for human coronavirus 229E (HCoV-229E), was identified as a major component of the Triton X-100-resistant membrane microdomain in human fibroblasts. The incubation of living fibroblasts with an anti-CD13 antibody on ice gave punctate labeling that was evenly distributed on the cell surface, but raising the temperature to 37°C before fixation caused aggregation of the labeling. The aggregated labeling of CD13 colocalized with caveolin-1 in most cells. The HCoV-229E virus particle showed a binding and redistribution pattern that was similar to that caused by the anti-CD13 antibody: the virus bound to the cell evenly when incubated on ice but became colocalized with caveolin-1 at 37°C; importantly, the virus also caused sequestration of CD13 to the caveolin-1-positive area. Electron microscopy confirmed that HCoV-229E was localized near or at the orifice of caveolae after incubation at 37°C. The depletion of plasmalemmal cholesterol with methyl β-cyclodextrin significantly reduced the HCoV-229E redistribution and subsequent infection. A caveolin-1 knockdown by RNA interference also reduced the HCoV-229E infection considerably. The results indicate that HCoV-229E first binds to CD13 in the Triton X-100-resistant microdomain, then clusters CD13 by cross-linking, and thereby reaches the caveolar region before entering cells.",
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Nomura, R, Kiyota, A, Suzaki, E, Kataoka, K, Ohe, Y, Miyamoto, K, Senda, T & Fujimoto, T 2004, 'Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae', Journal of Virology, vol. 78, no. 16, pp. 8701-8708. https://doi.org/10.1128/JVI.78.16.8701-8708.2004

Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae. / Nomura, Ryuji; Kiyota, Asuka; Suzaki, Etsuko; Kataoka, Katsuko; Ohe, Yoshihide; Miyamoto, Kaoru; Senda, Takao; Fujimoto, Toyoshi.

In: Journal of Virology, Vol. 78, No. 16, 01.08.2004, p. 8701-8708.

Research output: Contribution to journalArticle

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AU - Nomura, Ryuji

AU - Kiyota, Asuka

AU - Suzaki, Etsuko

AU - Kataoka, Katsuko

AU - Ohe, Yoshihide

AU - Miyamoto, Kaoru

AU - Senda, Takao

AU - Fujimoto, Toyoshi

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