TY - JOUR
T1 - Human herpesvirus 6 envelope cholesterol is required for virus entry
AU - Huang, Honglan
AU - Li, Yongmei
AU - Sadaoka, Tomohiko
AU - Tang, Huanmin
AU - Yamamoto, Takahito
AU - Yamanishi, Koichi
AU - Mori, Yasuko
PY - 2006/2
Y1 - 2006/2
N2 - In this study, the role of cholesterol in the envelope of human herpesvirus 6 (HHV-6) was examined by using methyl-β-cyclodextrin (MβCD) depletion. When cholesterol was removed from HHV-6 virions with MβCD, infectivity was abolished, but it could be rescued by the addition of exogenous cholesterol. HHV-6 binding was affected slightly by MβCD treatment. In contrast, envelope cholesterol depletion markedly affected HHV-6 infectivity and HHV-6-induced cell fusion. These results suggest that the cholesterol present in the HHV-6 envelope plays a prominent role in the fusion process and is a key component in viral entry.
AB - In this study, the role of cholesterol in the envelope of human herpesvirus 6 (HHV-6) was examined by using methyl-β-cyclodextrin (MβCD) depletion. When cholesterol was removed from HHV-6 virions with MβCD, infectivity was abolished, but it could be rescued by the addition of exogenous cholesterol. HHV-6 binding was affected slightly by MβCD treatment. In contrast, envelope cholesterol depletion markedly affected HHV-6 infectivity and HHV-6-induced cell fusion. These results suggest that the cholesterol present in the HHV-6 envelope plays a prominent role in the fusion process and is a key component in viral entry.
UR - https://www.scopus.com/pages/publications/31644438190
UR - https://www.scopus.com/pages/publications/31644438190#tab=citedBy
U2 - 10.1099/vir.0.81551-0
DO - 10.1099/vir.0.81551-0
M3 - Article
C2 - 16432012
AN - SCOPUS:31644438190
SN - 0022-1317
VL - 87
SP - 277
EP - 285
JO - Journal of General Virology
JF - Journal of General Virology
IS - 2
ER -