Human herpesvirus 6 infection of human epidermal cell line: Pathogenesis of skin manifestations

Tetsushi Yoshikawa, Fumi Goshima, Shiho Akimoto, Takao Ozaki, Takuya Iwasaki, Takeshi Kurata, Yoshizo Asano, Yukihiro Nishiyama

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV-6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV-6. HHV-6 causes abortive infection in the A431 cells, because neither late antigen (OHV-3 antigen) nor progeny virus is produced. Maximum levels of HHV-6 antigen (IEA/ex3 antigen)-positive cells (36.4%) were observed 48 hr after viral infection. Cocultivation of HHV-6-infected cord blood mononuclear cells with A431 cells was necessary for the establishment of a sufficient level of viral infection. Cell-to-cell contact between the infected cord blood mononuclear cells and A431 cells was crucial for increasing infection efficiency. To determine the biological effect of HHV-6 infection, flow cytometric analysis was carried out in HHV-6- and mock-infected A431 cells. Although no alteration was observed in VCAM-1 and ELAM-1 expression, that of HLA-ABC, HLA-DR, and ICAM-1 was upregulated after infection with HHV-6.

Original languageEnglish
Pages (from-to)62-68
Number of pages7
JournalJournal of Medical Virology
Issue number1
Publication statusPublished - 01-09-2003

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases


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