Abstract
In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV-6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV-6. HHV-6 causes abortive infection in the A431 cells, because neither late antigen (OHV-3 antigen) nor progeny virus is produced. Maximum levels of HHV-6 antigen (IEA/ex3 antigen)-positive cells (36.4%) were observed 48 hr after viral infection. Cocultivation of HHV-6-infected cord blood mononuclear cells with A431 cells was necessary for the establishment of a sufficient level of viral infection. Cell-to-cell contact between the infected cord blood mononuclear cells and A431 cells was crucial for increasing infection efficiency. To determine the biological effect of HHV-6 infection, flow cytometric analysis was carried out in HHV-6- and mock-infected A431 cells. Although no alteration was observed in VCAM-1 and ELAM-1 expression, that of HLA-ABC, HLA-DR, and ICAM-1 was upregulated after infection with HHV-6.
| Original language | English |
|---|---|
| Pages (from-to) | 62-68 |
| Number of pages | 7 |
| Journal | Journal of Medical Virology |
| Volume | 71 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 01-09-2003 |
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All Science Journal Classification (ASJC) codes
- Virology
- Infectious Diseases
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Human herpesvirus 6 infection of human epidermal cell line : Pathogenesis of skin manifestations. / Yoshikawa, Tetsushi; Goshima, Fumi; Akimoto, Shiho; Ozaki, Takao; Iwasaki, Takuya; Kurata, Takeshi; Asano, Yoshizo; Nishiyama, Yukihiro.
In: Journal of Medical Virology, Vol. 71, No. 1, 01.09.2003, p. 62-68.Research output: Contribution to journal › Article
TY - JOUR
T1 - Human herpesvirus 6 infection of human epidermal cell line
T2 - Pathogenesis of skin manifestations
AU - Yoshikawa, Tetsushi
AU - Goshima, Fumi
AU - Akimoto, Shiho
AU - Ozaki, Takao
AU - Iwasaki, Takuya
AU - Kurata, Takeshi
AU - Asano, Yoshizo
AU - Nishiyama, Yukihiro
PY - 2003/9/1
Y1 - 2003/9/1
N2 - In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV-6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV-6. HHV-6 causes abortive infection in the A431 cells, because neither late antigen (OHV-3 antigen) nor progeny virus is produced. Maximum levels of HHV-6 antigen (IEA/ex3 antigen)-positive cells (36.4%) were observed 48 hr after viral infection. Cocultivation of HHV-6-infected cord blood mononuclear cells with A431 cells was necessary for the establishment of a sufficient level of viral infection. Cell-to-cell contact between the infected cord blood mononuclear cells and A431 cells was crucial for increasing infection efficiency. To determine the biological effect of HHV-6 infection, flow cytometric analysis was carried out in HHV-6- and mock-infected A431 cells. Although no alteration was observed in VCAM-1 and ELAM-1 expression, that of HLA-ABC, HLA-DR, and ICAM-1 was upregulated after infection with HHV-6.
AB - In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV-6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV-6. HHV-6 causes abortive infection in the A431 cells, because neither late antigen (OHV-3 antigen) nor progeny virus is produced. Maximum levels of HHV-6 antigen (IEA/ex3 antigen)-positive cells (36.4%) were observed 48 hr after viral infection. Cocultivation of HHV-6-infected cord blood mononuclear cells with A431 cells was necessary for the establishment of a sufficient level of viral infection. Cell-to-cell contact between the infected cord blood mononuclear cells and A431 cells was crucial for increasing infection efficiency. To determine the biological effect of HHV-6 infection, flow cytometric analysis was carried out in HHV-6- and mock-infected A431 cells. Although no alteration was observed in VCAM-1 and ELAM-1 expression, that of HLA-ABC, HLA-DR, and ICAM-1 was upregulated after infection with HHV-6.
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UR - http://www.scopus.com/inward/citedby.url?scp=0141794182&partnerID=8YFLogxK
U2 - 10.1002/jmv.10461
DO - 10.1002/jmv.10461
M3 - Article
VL - 71
SP - 62
EP - 68
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 1
ER -