Human herpesvirus 6 infection of human epidermal cell line

Pathogenesis of skin manifestations

Tetsushi Yoshikawa, Fumi Goshima, Shiho Akimoto, Takao Ozaki, Takuya Iwasaki, Takeshi Kurata, Yoshizo Asano, Yukihiro Nishiyama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV-6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV-6. HHV-6 causes abortive infection in the A431 cells, because neither late antigen (OHV-3 antigen) nor progeny virus is produced. Maximum levels of HHV-6 antigen (IEA/ex3 antigen)-positive cells (36.4%) were observed 48 hr after viral infection. Cocultivation of HHV-6-infected cord blood mononuclear cells with A431 cells was necessary for the establishment of a sufficient level of viral infection. Cell-to-cell contact between the infected cord blood mononuclear cells and A431 cells was crucial for increasing infection efficiency. To determine the biological effect of HHV-6 infection, flow cytometric analysis was carried out in HHV-6- and mock-infected A431 cells. Although no alteration was observed in VCAM-1 and ELAM-1 expression, that of HLA-ABC, HLA-DR, and ICAM-1 was upregulated after infection with HHV-6.

Original languageEnglish
Pages (from-to)62-68
Number of pages7
JournalJournal of Medical Virology
Volume71
Issue number1
DOIs
Publication statusPublished - 01-09-2003

Fingerprint

Human Herpesvirus 6
Skin Manifestations
Herpesviridae Infections
Cell Line
Antigens
Virus Diseases
Fetal Blood
Blood Cells
Infection
E-Selectin
Vascular Cell Adhesion Molecule-1
HLA-DR Antigens
Intercellular Adhesion Molecule-1
Coculture Techniques
Viruses
Skin

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Yoshikawa, Tetsushi ; Goshima, Fumi ; Akimoto, Shiho ; Ozaki, Takao ; Iwasaki, Takuya ; Kurata, Takeshi ; Asano, Yoshizo ; Nishiyama, Yukihiro. / Human herpesvirus 6 infection of human epidermal cell line : Pathogenesis of skin manifestations. In: Journal of Medical Virology. 2003 ; Vol. 71, No. 1. pp. 62-68.
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abstract = "In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV-6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV-6. HHV-6 causes abortive infection in the A431 cells, because neither late antigen (OHV-3 antigen) nor progeny virus is produced. Maximum levels of HHV-6 antigen (IEA/ex3 antigen)-positive cells (36.4{\%}) were observed 48 hr after viral infection. Cocultivation of HHV-6-infected cord blood mononuclear cells with A431 cells was necessary for the establishment of a sufficient level of viral infection. Cell-to-cell contact between the infected cord blood mononuclear cells and A431 cells was crucial for increasing infection efficiency. To determine the biological effect of HHV-6 infection, flow cytometric analysis was carried out in HHV-6- and mock-infected A431 cells. Although no alteration was observed in VCAM-1 and ELAM-1 expression, that of HLA-ABC, HLA-DR, and ICAM-1 was upregulated after infection with HHV-6.",
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Yoshikawa, T, Goshima, F, Akimoto, S, Ozaki, T, Iwasaki, T, Kurata, T, Asano, Y & Nishiyama, Y 2003, 'Human herpesvirus 6 infection of human epidermal cell line: Pathogenesis of skin manifestations', Journal of Medical Virology, vol. 71, no. 1, pp. 62-68. https://doi.org/10.1002/jmv.10461

Human herpesvirus 6 infection of human epidermal cell line : Pathogenesis of skin manifestations. / Yoshikawa, Tetsushi; Goshima, Fumi; Akimoto, Shiho; Ozaki, Takao; Iwasaki, Takuya; Kurata, Takeshi; Asano, Yoshizo; Nishiyama, Yukihiro.

In: Journal of Medical Virology, Vol. 71, No. 1, 01.09.2003, p. 62-68.

Research output: Contribution to journalArticle

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AU - Yoshikawa, Tetsushi

AU - Goshima, Fumi

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AU - Ozaki, Takao

AU - Iwasaki, Takuya

AU - Kurata, Takeshi

AU - Asano, Yoshizo

AU - Nishiyama, Yukihiro

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N2 - In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV-6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV-6. HHV-6 causes abortive infection in the A431 cells, because neither late antigen (OHV-3 antigen) nor progeny virus is produced. Maximum levels of HHV-6 antigen (IEA/ex3 antigen)-positive cells (36.4%) were observed 48 hr after viral infection. Cocultivation of HHV-6-infected cord blood mononuclear cells with A431 cells was necessary for the establishment of a sufficient level of viral infection. Cell-to-cell contact between the infected cord blood mononuclear cells and A431 cells was crucial for increasing infection efficiency. To determine the biological effect of HHV-6 infection, flow cytometric analysis was carried out in HHV-6- and mock-infected A431 cells. Although no alteration was observed in VCAM-1 and ELAM-1 expression, that of HLA-ABC, HLA-DR, and ICAM-1 was upregulated after infection with HHV-6.

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