Human herpesvirus 6 latently infects mononuclear cells but not liver tissue

Tetsushi Yoshikawa, Kyoko Suzuki, Masaru Ihira, Hiroshi Furukawa, Sadao Suga, Takuya Iwasaki, Takeshi Kurata, Katsuhiro Asonuma, Koichi Tanaka, Yoshizo Asano

Research output: Contribution to journalArticle

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Abstract

Aim - To investigate whether human herpesvirus 6 (HHV-6) can cause latent infection of liver tissue. Methods - Peripheral blood and liver tissue were coRRected from 25 living related liver transplant recipients at the time of transplantation. An avidin-biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to detect HHV-6 DNA in the liver tissue and mononuclear cells. Variant of HHV-6 was determined by the presence of the Hind III site in a second PCR product. Results - Immunohistochemical analysis for HHV-6 antigen was negative in all the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA was detected in peripheral blood mononuclear cells in nine of 25 recipients. All nine HHV-6 identified in the mononuclear cells were variant B. Conclusions - HHV-6 variant B latently infects mononuclear cells but not liver tissue.

Original languageEnglish
Pages (from-to)65-67
Number of pages3
JournalJournal of Clinical Pathology
Volume52
Issue number1
DOIs
Publication statusPublished - 01-01-1999

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Human Herpesvirus 6
Liver
Antigens
Polymerase Chain Reaction
DNA Viruses
Avidin
DNA
Biotin
Peroxidase
Blood Cells
Transplantation

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Yoshikawa, Tetsushi ; Suzuki, Kyoko ; Ihira, Masaru ; Furukawa, Hiroshi ; Suga, Sadao ; Iwasaki, Takuya ; Kurata, Takeshi ; Asonuma, Katsuhiro ; Tanaka, Koichi ; Asano, Yoshizo. / Human herpesvirus 6 latently infects mononuclear cells but not liver tissue. In: Journal of Clinical Pathology. 1999 ; Vol. 52, No. 1. pp. 65-67.
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abstract = "Aim - To investigate whether human herpesvirus 6 (HHV-6) can cause latent infection of liver tissue. Methods - Peripheral blood and liver tissue were coRRected from 25 living related liver transplant recipients at the time of transplantation. An avidin-biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to detect HHV-6 DNA in the liver tissue and mononuclear cells. Variant of HHV-6 was determined by the presence of the Hind III site in a second PCR product. Results - Immunohistochemical analysis for HHV-6 antigen was negative in all the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA was detected in peripheral blood mononuclear cells in nine of 25 recipients. All nine HHV-6 identified in the mononuclear cells were variant B. Conclusions - HHV-6 variant B latently infects mononuclear cells but not liver tissue.",
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Yoshikawa, T, Suzuki, K, Ihira, M, Furukawa, H, Suga, S, Iwasaki, T, Kurata, T, Asonuma, K, Tanaka, K & Asano, Y 1999, 'Human herpesvirus 6 latently infects mononuclear cells but not liver tissue', Journal of Clinical Pathology, vol. 52, no. 1, pp. 65-67. https://doi.org/10.1136/jcp.52.1.65

Human herpesvirus 6 latently infects mononuclear cells but not liver tissue. / Yoshikawa, Tetsushi; Suzuki, Kyoko; Ihira, Masaru; Furukawa, Hiroshi; Suga, Sadao; Iwasaki, Takuya; Kurata, Takeshi; Asonuma, Katsuhiro; Tanaka, Koichi; Asano, Yoshizo.

In: Journal of Clinical Pathology, Vol. 52, No. 1, 01.01.1999, p. 65-67.

Research output: Contribution to journalArticle

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T1 - Human herpesvirus 6 latently infects mononuclear cells but not liver tissue

AU - Yoshikawa, Tetsushi

AU - Suzuki, Kyoko

AU - Ihira, Masaru

AU - Furukawa, Hiroshi

AU - Suga, Sadao

AU - Iwasaki, Takuya

AU - Kurata, Takeshi

AU - Asonuma, Katsuhiro

AU - Tanaka, Koichi

AU - Asano, Yoshizo

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Aim - To investigate whether human herpesvirus 6 (HHV-6) can cause latent infection of liver tissue. Methods - Peripheral blood and liver tissue were coRRected from 25 living related liver transplant recipients at the time of transplantation. An avidin-biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to detect HHV-6 DNA in the liver tissue and mononuclear cells. Variant of HHV-6 was determined by the presence of the Hind III site in a second PCR product. Results - Immunohistochemical analysis for HHV-6 antigen was negative in all the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA was detected in peripheral blood mononuclear cells in nine of 25 recipients. All nine HHV-6 identified in the mononuclear cells were variant B. Conclusions - HHV-6 variant B latently infects mononuclear cells but not liver tissue.

AB - Aim - To investigate whether human herpesvirus 6 (HHV-6) can cause latent infection of liver tissue. Methods - Peripheral blood and liver tissue were coRRected from 25 living related liver transplant recipients at the time of transplantation. An avidin-biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to detect HHV-6 DNA in the liver tissue and mononuclear cells. Variant of HHV-6 was determined by the presence of the Hind III site in a second PCR product. Results - Immunohistochemical analysis for HHV-6 antigen was negative in all the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA was detected in peripheral blood mononuclear cells in nine of 25 recipients. All nine HHV-6 identified in the mononuclear cells were variant B. Conclusions - HHV-6 variant B latently infects mononuclear cells but not liver tissue.

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