Human immunodeficiency virus-1 Nef suppresses Hsp70-mediated Tat activation

Ryuichi Sugiyama, Haruki Naganuma, Hironori Nishitsuji, Hiroshi Takaku

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


The human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) contains binding sites for several host transcription factors that contribute to HIV-1 gene expression. Although previous reports have indicated that HIV-1 Nef positively or negatively regulates HIV-1 gene expression, the precise molecular mechanisms by which this occurs remain largely unknown. In this study, we report that Nef suppressed LTR-driven transcription only in the presence of HIV-1 Tat, which was localized to the cytoplasm and degraded by the proteasome. However, the depletion of Hsp70 was found to reduce the suppressive effect of Nef on HIV-1 gene expression. These results suggest that Nef suppresses Hsp70-mediated HIV-1 Tat activation.

Original languageEnglish
Pages (from-to)3367-3371
Number of pages5
JournalFEBS Letters
Issue number21
Publication statusPublished - 04-11-2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


Dive into the research topics of 'Human immunodeficiency virus-1 Nef suppresses Hsp70-mediated Tat activation'. Together they form a unique fingerprint.

Cite this