TY - JOUR
T1 - Human immunodeficiency virus-1 Nef suppresses Hsp70-mediated Tat activation
AU - Sugiyama, Ryuichi
AU - Naganuma, Haruki
AU - Nishitsuji, Hironori
AU - Takaku, Hiroshi
N1 - Funding Information:
We thank Drs. M. Kannagi and T. Hayashi for providing pNef-V5 and its mutants, Dr. I.S.Y. Chen for providing pNL4-3lucΔenv and Dr. A. Ryo for providing pCMV-Myc-Ubi. This work was supported in part by a Grant-in-Aid for High Technology Research (no. 09309011) from the Ministry of Education, Science, Sports, and Culture in Japan, by a Grant-in-Aid for AIDS research from the Ministry of Health, Labor, and Welfare in Japan, and by a Grant from the Ministry of Education, Culture, Sports, Science, and Technology in Japan (MEXT) as part of the Strategic Research Foundation Grant-Aided Project for Private Universities.
PY - 2011/11/4
Y1 - 2011/11/4
N2 - The human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) contains binding sites for several host transcription factors that contribute to HIV-1 gene expression. Although previous reports have indicated that HIV-1 Nef positively or negatively regulates HIV-1 gene expression, the precise molecular mechanisms by which this occurs remain largely unknown. In this study, we report that Nef suppressed LTR-driven transcription only in the presence of HIV-1 Tat, which was localized to the cytoplasm and degraded by the proteasome. However, the depletion of Hsp70 was found to reduce the suppressive effect of Nef on HIV-1 gene expression. These results suggest that Nef suppresses Hsp70-mediated HIV-1 Tat activation.
AB - The human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) contains binding sites for several host transcription factors that contribute to HIV-1 gene expression. Although previous reports have indicated that HIV-1 Nef positively or negatively regulates HIV-1 gene expression, the precise molecular mechanisms by which this occurs remain largely unknown. In this study, we report that Nef suppressed LTR-driven transcription only in the presence of HIV-1 Tat, which was localized to the cytoplasm and degraded by the proteasome. However, the depletion of Hsp70 was found to reduce the suppressive effect of Nef on HIV-1 gene expression. These results suggest that Nef suppresses Hsp70-mediated HIV-1 Tat activation.
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U2 - 10.1016/j.febslet.2011.09.029
DO - 10.1016/j.febslet.2011.09.029
M3 - Article
C2 - 21970979
AN - SCOPUS:80255140478
SN - 0014-5793
VL - 585
SP - 3367
EP - 3371
JO - FEBS Letters
JF - FEBS Letters
IS - 21
ER -
