Human Immunodeficiency Virus Type 1 hnRNP A/B-Dependent Exonic Splicing Silencer ESSV Antagonizes Binding of U2AF65 to Viral Polypyrimidine Tracts

Jeffrey K. Domsic, Yibin Wang, Akila Mayeda, Adrian R. Krainer, C. Martin Stoltzfus

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Human immunodeficiency virus type 1 (HIV-1) exonic splicing silencers (ESSs) inhibit production of certain spliced viral RNAs by repressing alternative splicing of the viral precursor RNA. Several HIV-1 ESSs interfere with spliceosome assembly by binding cellular hnRNP A/B proteins. Here, we have further characterized the mechanism of splicing repression using a representative HIV-1 hnRNP A/B-dependent ESS, ESSV, which regulates splicing at the vpr 3′ splice site. We show that hnRNP A/B proteins bound to ESSV are necessary to inhibit E complex assembly by competing with the binding of U2AF65 to the polypyrimidine tracts of repressed 3′ splice sites. We further show evidence suggesting that U1 snRNP binds the 5′ splice site despite an almost complete block of splicing by ESSV. Possible splicing-independent functions of U1 snRNP-5′ splice site interactions during virus replication are discussed.

Original languageEnglish
Pages (from-to)8762-8772
Number of pages11
JournalMolecular and Cellular Biology
Volume23
Issue number23
DOIs
Publication statusPublished - 12-2003

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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