Human immunodeficiency virus type 1 subtype C exhibits higher transactivation activity of tat than subtypes B and E

Takeshi Kurosu, Tetsu Mukai, Satoshi Komoto, Madiha S. Ibrahim, Yong Gang Li, Takeshi Kobayashi, Shoutaro Tsuji, Kazuyoshi Ikuta

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Although human immunodeficiency virus type 1 (HIV-1) subtypes C and E are expanding faster and seem to be of greater global significance than HIV-1 subtype B, there is only little information about Tat activity of such non-B subtypes. Here, we showed evidence that subtype C Tat exhibits higher transcriptional activity from the HIV-1 long-terminal repeat (LTR) in a human T-cell line, compared with subtypes B and E. This higher activity of subtype C Tat was not due to the LTR, but to the Tat sequence variability. We examined three candidate regions with sequence for the higher activity of subtype C Tat, such as the cysteine-rich motif, the basic domain, and the 2nd exon. The results showed that the variation in subtype C Tat at two amino acid residues, Ser 57 and Glu 63 in stead of Arg 57 and Gln 63 in subtypes B and E, within and close to the basic domain were involved in the higher activity of subtype C Tat. This variation did not affect its nuclear localization activity. Thus, there may be a significant advantage for the high Tat activity on subtype C replication.

Original languageEnglish
Pages (from-to)787-799
Number of pages13
JournalMICROBIOLOGY and IMMUNOLOGY
Volume46
Issue number11
DOIs
Publication statusPublished - 01-01-2002

Fingerprint

Transcriptional Activation
HIV-1
HIV Long Terminal Repeat
Terminal Repeat Sequences
Cysteine
Exons
T-Lymphocytes
Amino Acids
Cell Line

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Kurosu, Takeshi ; Mukai, Tetsu ; Komoto, Satoshi ; Ibrahim, Madiha S. ; Li, Yong Gang ; Kobayashi, Takeshi ; Tsuji, Shoutaro ; Ikuta, Kazuyoshi. / Human immunodeficiency virus type 1 subtype C exhibits higher transactivation activity of tat than subtypes B and E. In: MICROBIOLOGY and IMMUNOLOGY. 2002 ; Vol. 46, No. 11. pp. 787-799.
@article{effdd12389b345a6b206a4911bfddcbd,
title = "Human immunodeficiency virus type 1 subtype C exhibits higher transactivation activity of tat than subtypes B and E",
abstract = "Although human immunodeficiency virus type 1 (HIV-1) subtypes C and E are expanding faster and seem to be of greater global significance than HIV-1 subtype B, there is only little information about Tat activity of such non-B subtypes. Here, we showed evidence that subtype C Tat exhibits higher transcriptional activity from the HIV-1 long-terminal repeat (LTR) in a human T-cell line, compared with subtypes B and E. This higher activity of subtype C Tat was not due to the LTR, but to the Tat sequence variability. We examined three candidate regions with sequence for the higher activity of subtype C Tat, such as the cysteine-rich motif, the basic domain, and the 2nd exon. The results showed that the variation in subtype C Tat at two amino acid residues, Ser 57 and Glu 63 in stead of Arg 57 and Gln 63 in subtypes B and E, within and close to the basic domain were involved in the higher activity of subtype C Tat. This variation did not affect its nuclear localization activity. Thus, there may be a significant advantage for the high Tat activity on subtype C replication.",
author = "Takeshi Kurosu and Tetsu Mukai and Satoshi Komoto and Ibrahim, {Madiha S.} and Li, {Yong Gang} and Takeshi Kobayashi and Shoutaro Tsuji and Kazuyoshi Ikuta",
year = "2002",
month = "1",
day = "1",
doi = "10.1111/j.1348-0421.2002.tb02766.x",
language = "English",
volume = "46",
pages = "787--799",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "11",

}

Human immunodeficiency virus type 1 subtype C exhibits higher transactivation activity of tat than subtypes B and E. / Kurosu, Takeshi; Mukai, Tetsu; Komoto, Satoshi; Ibrahim, Madiha S.; Li, Yong Gang; Kobayashi, Takeshi; Tsuji, Shoutaro; Ikuta, Kazuyoshi.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 46, No. 11, 01.01.2002, p. 787-799.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Human immunodeficiency virus type 1 subtype C exhibits higher transactivation activity of tat than subtypes B and E

AU - Kurosu, Takeshi

AU - Mukai, Tetsu

AU - Komoto, Satoshi

AU - Ibrahim, Madiha S.

AU - Li, Yong Gang

AU - Kobayashi, Takeshi

AU - Tsuji, Shoutaro

AU - Ikuta, Kazuyoshi

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Although human immunodeficiency virus type 1 (HIV-1) subtypes C and E are expanding faster and seem to be of greater global significance than HIV-1 subtype B, there is only little information about Tat activity of such non-B subtypes. Here, we showed evidence that subtype C Tat exhibits higher transcriptional activity from the HIV-1 long-terminal repeat (LTR) in a human T-cell line, compared with subtypes B and E. This higher activity of subtype C Tat was not due to the LTR, but to the Tat sequence variability. We examined three candidate regions with sequence for the higher activity of subtype C Tat, such as the cysteine-rich motif, the basic domain, and the 2nd exon. The results showed that the variation in subtype C Tat at two amino acid residues, Ser 57 and Glu 63 in stead of Arg 57 and Gln 63 in subtypes B and E, within and close to the basic domain were involved in the higher activity of subtype C Tat. This variation did not affect its nuclear localization activity. Thus, there may be a significant advantage for the high Tat activity on subtype C replication.

AB - Although human immunodeficiency virus type 1 (HIV-1) subtypes C and E are expanding faster and seem to be of greater global significance than HIV-1 subtype B, there is only little information about Tat activity of such non-B subtypes. Here, we showed evidence that subtype C Tat exhibits higher transcriptional activity from the HIV-1 long-terminal repeat (LTR) in a human T-cell line, compared with subtypes B and E. This higher activity of subtype C Tat was not due to the LTR, but to the Tat sequence variability. We examined three candidate regions with sequence for the higher activity of subtype C Tat, such as the cysteine-rich motif, the basic domain, and the 2nd exon. The results showed that the variation in subtype C Tat at two amino acid residues, Ser 57 and Glu 63 in stead of Arg 57 and Gln 63 in subtypes B and E, within and close to the basic domain were involved in the higher activity of subtype C Tat. This variation did not affect its nuclear localization activity. Thus, there may be a significant advantage for the high Tat activity on subtype C replication.

UR - http://www.scopus.com/inward/record.url?scp=0036443904&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036443904&partnerID=8YFLogxK

U2 - 10.1111/j.1348-0421.2002.tb02766.x

DO - 10.1111/j.1348-0421.2002.tb02766.x

M3 - Article

C2 - 12516777

AN - SCOPUS:0036443904

VL - 46

SP - 787

EP - 799

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 11

ER -