Human infection with Plasmodium knowlesi on the Laos-Vietnam border 11 Medical and Health Sciences 1108 Medical Microbiology

Tiengkham Pongvongsa, Richard Culleton, Hoang Ha, Le Thanh, Panom Phongmany, Ron P. Marchand, Satoru Kawai, Kazuhiko Moji, Shusuke Nakazawa, Yoshimasa Maeno

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Border malaria in the Greater Mekong region of Southeast Asia poses a serious threat to the health of the ethnic minority populations of the region. Traditionally thought to be caused primarily by the malaria parasites Plasmodium falciparum and Plasmodium vivax, recently a zoonotic parasite, Plasmodium knowlesi, has been identified in some countries of the region. The presence of this parasite poses a challenge to malaria control programmes, as it is maintained in a zoonotic reservoir of forest-dwelling macaque monkeys. Methods: A cross-sectional malaria parasite species prevalence survey was conducted along the Laos-Vietnam border in the central part of the two countries. Human blood samples were collected from Savannakhet in Laos and Quang Tri in Vietnam between August and October 2010 and assayed for the presence of human malaria parasite species and P. knowlesi. A PCR targeting the 18S small subunit ribosomal RNA gene and circumsporozoite protein gene was used for Plasmodium species identification. Results: Nine cases of P. knowlesi were detected by PCR in blood samples from the Laos side and three from the Vietnam side. All P. knowlesi infections were found in co-infection with P. vivax, with some triple infections of P. knowlesi, P. vivax and P. falciparum detected in Laos. Phylogenetic analysis of these parasites suggests that P. knowlesi is circulating in the Laos-Vietnam border region. Conclusion: This report shows that P. knowlesi is transmited on both sides of the Vietnam-Laos border. Continued monitoring of the range and prevalence of P. knowlesi on both the sides of Laos-Vietnam border is of importance to the National Malaria Control Programmes of both countries.

Original languageEnglish
Article number33
JournalTropical Medicine and Health
Volume46
Issue number1
DOIs
Publication statusPublished - 18-09-2018

Fingerprint

Plasmodium knowlesi
Laos
Vietnam
Microbiology
Malaria
Parasites
Health
Infection
Plasmodium vivax
Zoonoses
Minority Health
Polymerase Chain Reaction
Southeastern Asia
Plasmodium
Falciparum Malaria
Macaca
Plasmodium falciparum
Coinfection
rRNA Genes
Haplorhini

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Pongvongsa, Tiengkham ; Culleton, Richard ; Ha, Hoang ; Thanh, Le ; Phongmany, Panom ; Marchand, Ron P. ; Kawai, Satoru ; Moji, Kazuhiko ; Nakazawa, Shusuke ; Maeno, Yoshimasa. / Human infection with Plasmodium knowlesi on the Laos-Vietnam border 11 Medical and Health Sciences 1108 Medical Microbiology. In: Tropical Medicine and Health. 2018 ; Vol. 46, No. 1.
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title = "Human infection with Plasmodium knowlesi on the Laos-Vietnam border 11 Medical and Health Sciences 1108 Medical Microbiology",
abstract = "Background: Border malaria in the Greater Mekong region of Southeast Asia poses a serious threat to the health of the ethnic minority populations of the region. Traditionally thought to be caused primarily by the malaria parasites Plasmodium falciparum and Plasmodium vivax, recently a zoonotic parasite, Plasmodium knowlesi, has been identified in some countries of the region. The presence of this parasite poses a challenge to malaria control programmes, as it is maintained in a zoonotic reservoir of forest-dwelling macaque monkeys. Methods: A cross-sectional malaria parasite species prevalence survey was conducted along the Laos-Vietnam border in the central part of the two countries. Human blood samples were collected from Savannakhet in Laos and Quang Tri in Vietnam between August and October 2010 and assayed for the presence of human malaria parasite species and P. knowlesi. A PCR targeting the 18S small subunit ribosomal RNA gene and circumsporozoite protein gene was used for Plasmodium species identification. Results: Nine cases of P. knowlesi were detected by PCR in blood samples from the Laos side and three from the Vietnam side. All P. knowlesi infections were found in co-infection with P. vivax, with some triple infections of P. knowlesi, P. vivax and P. falciparum detected in Laos. Phylogenetic analysis of these parasites suggests that P. knowlesi is circulating in the Laos-Vietnam border region. Conclusion: This report shows that P. knowlesi is transmited on both sides of the Vietnam-Laos border. Continued monitoring of the range and prevalence of P. knowlesi on both the sides of Laos-Vietnam border is of importance to the National Malaria Control Programmes of both countries.",
author = "Tiengkham Pongvongsa and Richard Culleton and Hoang Ha and Le Thanh and Panom Phongmany and Marchand, {Ron P.} and Satoru Kawai and Kazuhiko Moji and Shusuke Nakazawa and Yoshimasa Maeno",
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Pongvongsa, T, Culleton, R, Ha, H, Thanh, L, Phongmany, P, Marchand, RP, Kawai, S, Moji, K, Nakazawa, S & Maeno, Y 2018, 'Human infection with Plasmodium knowlesi on the Laos-Vietnam border 11 Medical and Health Sciences 1108 Medical Microbiology', Tropical Medicine and Health, vol. 46, no. 1, 33. https://doi.org/10.1186/s41182-018-0116-7

Human infection with Plasmodium knowlesi on the Laos-Vietnam border 11 Medical and Health Sciences 1108 Medical Microbiology. / Pongvongsa, Tiengkham; Culleton, Richard; Ha, Hoang; Thanh, Le; Phongmany, Panom; Marchand, Ron P.; Kawai, Satoru; Moji, Kazuhiko; Nakazawa, Shusuke; Maeno, Yoshimasa.

In: Tropical Medicine and Health, Vol. 46, No. 1, 33, 18.09.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Human infection with Plasmodium knowlesi on the Laos-Vietnam border 11 Medical and Health Sciences 1108 Medical Microbiology

AU - Pongvongsa, Tiengkham

AU - Culleton, Richard

AU - Ha, Hoang

AU - Thanh, Le

AU - Phongmany, Panom

AU - Marchand, Ron P.

AU - Kawai, Satoru

AU - Moji, Kazuhiko

AU - Nakazawa, Shusuke

AU - Maeno, Yoshimasa

PY - 2018/9/18

Y1 - 2018/9/18

N2 - Background: Border malaria in the Greater Mekong region of Southeast Asia poses a serious threat to the health of the ethnic minority populations of the region. Traditionally thought to be caused primarily by the malaria parasites Plasmodium falciparum and Plasmodium vivax, recently a zoonotic parasite, Plasmodium knowlesi, has been identified in some countries of the region. The presence of this parasite poses a challenge to malaria control programmes, as it is maintained in a zoonotic reservoir of forest-dwelling macaque monkeys. Methods: A cross-sectional malaria parasite species prevalence survey was conducted along the Laos-Vietnam border in the central part of the two countries. Human blood samples were collected from Savannakhet in Laos and Quang Tri in Vietnam between August and October 2010 and assayed for the presence of human malaria parasite species and P. knowlesi. A PCR targeting the 18S small subunit ribosomal RNA gene and circumsporozoite protein gene was used for Plasmodium species identification. Results: Nine cases of P. knowlesi were detected by PCR in blood samples from the Laos side and three from the Vietnam side. All P. knowlesi infections were found in co-infection with P. vivax, with some triple infections of P. knowlesi, P. vivax and P. falciparum detected in Laos. Phylogenetic analysis of these parasites suggests that P. knowlesi is circulating in the Laos-Vietnam border region. Conclusion: This report shows that P. knowlesi is transmited on both sides of the Vietnam-Laos border. Continued monitoring of the range and prevalence of P. knowlesi on both the sides of Laos-Vietnam border is of importance to the National Malaria Control Programmes of both countries.

AB - Background: Border malaria in the Greater Mekong region of Southeast Asia poses a serious threat to the health of the ethnic minority populations of the region. Traditionally thought to be caused primarily by the malaria parasites Plasmodium falciparum and Plasmodium vivax, recently a zoonotic parasite, Plasmodium knowlesi, has been identified in some countries of the region. The presence of this parasite poses a challenge to malaria control programmes, as it is maintained in a zoonotic reservoir of forest-dwelling macaque monkeys. Methods: A cross-sectional malaria parasite species prevalence survey was conducted along the Laos-Vietnam border in the central part of the two countries. Human blood samples were collected from Savannakhet in Laos and Quang Tri in Vietnam between August and October 2010 and assayed for the presence of human malaria parasite species and P. knowlesi. A PCR targeting the 18S small subunit ribosomal RNA gene and circumsporozoite protein gene was used for Plasmodium species identification. Results: Nine cases of P. knowlesi were detected by PCR in blood samples from the Laos side and three from the Vietnam side. All P. knowlesi infections were found in co-infection with P. vivax, with some triple infections of P. knowlesi, P. vivax and P. falciparum detected in Laos. Phylogenetic analysis of these parasites suggests that P. knowlesi is circulating in the Laos-Vietnam border region. Conclusion: This report shows that P. knowlesi is transmited on both sides of the Vietnam-Laos border. Continued monitoring of the range and prevalence of P. knowlesi on both the sides of Laos-Vietnam border is of importance to the National Malaria Control Programmes of both countries.

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