Human ISLET isolation in 104 consecutive cases

Factors affecting isolation success

Pierre Y. Benhamou, Philip C. Watt, Yoko Mullen, Sue Ingles, Yasuo Watanabe, Yuji Nomura, Christine Hober, Masaaki Miyamoto, Takashi Kenmochi, Edward P. Passaro, Michael J. Zinner, F. Charles Brunicardi

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

One of the major steps toward successful islet transplantation for the treatment of type diabetes is to obtain islets of sufficient number and viability. Using a standardized method of isolating islets, the goal of this study was to analyze the factors influencing the outcome of islet isolation. A total of 104 cadaveric human pancrcata were processed for islets by the same team. Data from the islet-processing charts were reviewed retrospectively. The two endpoints were the recovery of islets, viable after 2 days of culture (group V=viable, group NV=non viable) and the islet yield. Viable islets were recovered in 61% of cases (n=63). Minimal blood glucose recorded during hospitalization was very significantly lower in group V (124±6 vs. 148±9, P=0.01). Lack of significant medical history in the donor was associated with better viability as compared with various donor predispositions (chi- 2 4.21, P=0.04). Cold ischemia time (8.1 ±0.6 hr in group V vs. 9.8±0.9 hr in group NV, P=0.07) and collagenase lot (5 lots tested, chi- 2 13.1, P=0.01) also affected the recovery of viable islets. Hospital time was shorter in group V (65.3 ± 6.8 vs. 80.9±17.9 hr, P=0.35). Multivariate logistic regression analyses of viable islet recovery identified minimal blood glucose (P=0.03) and collagenase lot (P=0.06) as the most significant risk factors. However, the best multivariate predictive model—which includes blood glucose, collagenase lot, donor age and surgical procurement team—correctly predicted 66.2% of cases only. Multivariate analysis of final islet yield designed hospitalization length, cardiorespiratory arrest, surgical procurement team, and collagenase lot as the best predictors. These data obtained in a large series of pancreata emphasized several donor and technical factors that should target the attention of islet transplant researchers in order to improve islet yield and viability.

Original languageEnglish
Pages (from-to)1804-1810
Number of pages7
JournalTransplantation
Volume57
Issue number12
DOIs
Publication statusPublished - 01-01-1994

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Collagenases
Blood Glucose
Hospitalization
Cold Ischemia
Islets of Langerhans Transplantation
Statistical Factor Analysis
Pancreas
Multivariate Analysis
Logistic Models
Regression Analysis
Research Personnel
Transplants
Therapeutics

All Science Journal Classification (ASJC) codes

  • Transplantation

Cite this

Benhamou, P. Y., Watt, P. C., Mullen, Y., Ingles, S., Watanabe, Y., Nomura, Y., ... Brunicardi, F. C. (1994). Human ISLET isolation in 104 consecutive cases: Factors affecting isolation success. Transplantation, 57(12), 1804-1810. https://doi.org/10.1097/00007890-199457120-00021
Benhamou, Pierre Y. ; Watt, Philip C. ; Mullen, Yoko ; Ingles, Sue ; Watanabe, Yasuo ; Nomura, Yuji ; Hober, Christine ; Miyamoto, Masaaki ; Kenmochi, Takashi ; Passaro, Edward P. ; Zinner, Michael J. ; Brunicardi, F. Charles. / Human ISLET isolation in 104 consecutive cases : Factors affecting isolation success. In: Transplantation. 1994 ; Vol. 57, No. 12. pp. 1804-1810.
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abstract = "One of the major steps toward successful islet transplantation for the treatment of type diabetes is to obtain islets of sufficient number and viability. Using a standardized method of isolating islets, the goal of this study was to analyze the factors influencing the outcome of islet isolation. A total of 104 cadaveric human pancrcata were processed for islets by the same team. Data from the islet-processing charts were reviewed retrospectively. The two endpoints were the recovery of islets, viable after 2 days of culture (group V=viable, group NV=non viable) and the islet yield. Viable islets were recovered in 61{\%} of cases (n=63). Minimal blood glucose recorded during hospitalization was very significantly lower in group V (124±6 vs. 148±9, P=0.01). Lack of significant medical history in the donor was associated with better viability as compared with various donor predispositions (chi- 2 4.21, P=0.04). Cold ischemia time (8.1 ±0.6 hr in group V vs. 9.8±0.9 hr in group NV, P=0.07) and collagenase lot (5 lots tested, chi- 2 13.1, P=0.01) also affected the recovery of viable islets. Hospital time was shorter in group V (65.3 ± 6.8 vs. 80.9±17.9 hr, P=0.35). Multivariate logistic regression analyses of viable islet recovery identified minimal blood glucose (P=0.03) and collagenase lot (P=0.06) as the most significant risk factors. However, the best multivariate predictive model—which includes blood glucose, collagenase lot, donor age and surgical procurement team—correctly predicted 66.2{\%} of cases only. Multivariate analysis of final islet yield designed hospitalization length, cardiorespiratory arrest, surgical procurement team, and collagenase lot as the best predictors. These data obtained in a large series of pancreata emphasized several donor and technical factors that should target the attention of islet transplant researchers in order to improve islet yield and viability.",
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Benhamou, PY, Watt, PC, Mullen, Y, Ingles, S, Watanabe, Y, Nomura, Y, Hober, C, Miyamoto, M, Kenmochi, T, Passaro, EP, Zinner, MJ & Brunicardi, FC 1994, 'Human ISLET isolation in 104 consecutive cases: Factors affecting isolation success', Transplantation, vol. 57, no. 12, pp. 1804-1810. https://doi.org/10.1097/00007890-199457120-00021

Human ISLET isolation in 104 consecutive cases : Factors affecting isolation success. / Benhamou, Pierre Y.; Watt, Philip C.; Mullen, Yoko; Ingles, Sue; Watanabe, Yasuo; Nomura, Yuji; Hober, Christine; Miyamoto, Masaaki; Kenmochi, Takashi; Passaro, Edward P.; Zinner, Michael J.; Brunicardi, F. Charles.

In: Transplantation, Vol. 57, No. 12, 01.01.1994, p. 1804-1810.

Research output: Contribution to journalArticle

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AU - Benhamou, Pierre Y.

AU - Watt, Philip C.

AU - Mullen, Yoko

AU - Ingles, Sue

AU - Watanabe, Yasuo

AU - Nomura, Yuji

AU - Hober, Christine

AU - Miyamoto, Masaaki

AU - Kenmochi, Takashi

AU - Passaro, Edward P.

AU - Zinner, Michael J.

AU - Brunicardi, F. Charles

PY - 1994/1/1

Y1 - 1994/1/1

N2 - One of the major steps toward successful islet transplantation for the treatment of type diabetes is to obtain islets of sufficient number and viability. Using a standardized method of isolating islets, the goal of this study was to analyze the factors influencing the outcome of islet isolation. A total of 104 cadaveric human pancrcata were processed for islets by the same team. Data from the islet-processing charts were reviewed retrospectively. The two endpoints were the recovery of islets, viable after 2 days of culture (group V=viable, group NV=non viable) and the islet yield. Viable islets were recovered in 61% of cases (n=63). Minimal blood glucose recorded during hospitalization was very significantly lower in group V (124±6 vs. 148±9, P=0.01). Lack of significant medical history in the donor was associated with better viability as compared with various donor predispositions (chi- 2 4.21, P=0.04). Cold ischemia time (8.1 ±0.6 hr in group V vs. 9.8±0.9 hr in group NV, P=0.07) and collagenase lot (5 lots tested, chi- 2 13.1, P=0.01) also affected the recovery of viable islets. Hospital time was shorter in group V (65.3 ± 6.8 vs. 80.9±17.9 hr, P=0.35). Multivariate logistic regression analyses of viable islet recovery identified minimal blood glucose (P=0.03) and collagenase lot (P=0.06) as the most significant risk factors. However, the best multivariate predictive model—which includes blood glucose, collagenase lot, donor age and surgical procurement team—correctly predicted 66.2% of cases only. Multivariate analysis of final islet yield designed hospitalization length, cardiorespiratory arrest, surgical procurement team, and collagenase lot as the best predictors. These data obtained in a large series of pancreata emphasized several donor and technical factors that should target the attention of islet transplant researchers in order to improve islet yield and viability.

AB - One of the major steps toward successful islet transplantation for the treatment of type diabetes is to obtain islets of sufficient number and viability. Using a standardized method of isolating islets, the goal of this study was to analyze the factors influencing the outcome of islet isolation. A total of 104 cadaveric human pancrcata were processed for islets by the same team. Data from the islet-processing charts were reviewed retrospectively. The two endpoints were the recovery of islets, viable after 2 days of culture (group V=viable, group NV=non viable) and the islet yield. Viable islets were recovered in 61% of cases (n=63). Minimal blood glucose recorded during hospitalization was very significantly lower in group V (124±6 vs. 148±9, P=0.01). Lack of significant medical history in the donor was associated with better viability as compared with various donor predispositions (chi- 2 4.21, P=0.04). Cold ischemia time (8.1 ±0.6 hr in group V vs. 9.8±0.9 hr in group NV, P=0.07) and collagenase lot (5 lots tested, chi- 2 13.1, P=0.01) also affected the recovery of viable islets. Hospital time was shorter in group V (65.3 ± 6.8 vs. 80.9±17.9 hr, P=0.35). Multivariate logistic regression analyses of viable islet recovery identified minimal blood glucose (P=0.03) and collagenase lot (P=0.06) as the most significant risk factors. However, the best multivariate predictive model—which includes blood glucose, collagenase lot, donor age and surgical procurement team—correctly predicted 66.2% of cases only. Multivariate analysis of final islet yield designed hospitalization length, cardiorespiratory arrest, surgical procurement team, and collagenase lot as the best predictors. These data obtained in a large series of pancreata emphasized several donor and technical factors that should target the attention of islet transplant researchers in order to improve islet yield and viability.

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Benhamou PY, Watt PC, Mullen Y, Ingles S, Watanabe Y, Nomura Y et al. Human ISLET isolation in 104 consecutive cases: Factors affecting isolation success. Transplantation. 1994 Jan 1;57(12):1804-1810. https://doi.org/10.1097/00007890-199457120-00021