Human RNPS1 and Its Associated Factors: A Versatile Alternative Pre-mRNA Splicing Regulator in Vivo

Eiji Sakashita, Sawako Tatsumi, Dieter Werner, Hitoshi Endo, Akira Maeda

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Human RNPS1 was originally purified and characterized as a pre-mRNA splicing activator, and its role in the postsplicing process has also been proposed recently. To search for factors that functionally interact with RNPS1, we performed a yeast two-hybrid screen with a human cDNA library. Four factors were identified: p54 (also called SRp54; a member of the SR protein family), human transformer 2β (hTra2β; an exonic splicing enhancer-binding protein), hLucA (a potential component of U1 snRNP), and pinin (also called DRS and MemA; a protein localized in nuclear speckles). The N-terminal region containing the serine-rich (S) domain, the central RNA recognition motif (RRM), and the C-terminal arginine/serine/proline-rich (RS/P) domain of RNPS1 interact with p54, pinin, and hTra2β, respectively. Protein-protein binding between RNPS1 and these factors was verified in vitro and in vivo. Overexpression of RNPS1 in HeLa cells induced exon skipping in a model β-globin pre-mRNA and a human tra-2β pre-mRNA. Coexpression of RNPS1 with p54 cooperatively stimulated exon inclusion in an ATP synthase γ-subunit pre-mRNA. The RS/P domain and RRM are necessary for the exon-skipping activity, whereas the S domain is important for the cooperative effect with p54. RNPS1 appears to be a versatile factor that regulates alternative splicing of a variety of pre-mRNAs.

Original languageEnglish
Pages (from-to)1174-1187
Number of pages14
JournalMolecular and Cellular Biology
Volume24
Issue number3
DOIs
Publication statusPublished - 01-02-2004

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RNA Precursors
Exons
Serine
U1 Small Nuclear Ribonucleoproteins
Proteins
Globins
Alternative Splicing
Gene Library
HeLa Cells
Proline
Protein Binding
Arginine
Carrier Proteins
Adenosine Triphosphate
Yeasts
RNA Recognition Motif

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Sakashita, Eiji ; Tatsumi, Sawako ; Werner, Dieter ; Endo, Hitoshi ; Maeda, Akira. / Human RNPS1 and Its Associated Factors : A Versatile Alternative Pre-mRNA Splicing Regulator in Vivo. In: Molecular and Cellular Biology. 2004 ; Vol. 24, No. 3. pp. 1174-1187.
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Human RNPS1 and Its Associated Factors : A Versatile Alternative Pre-mRNA Splicing Regulator in Vivo. / Sakashita, Eiji; Tatsumi, Sawako; Werner, Dieter; Endo, Hitoshi; Maeda, Akira.

In: Molecular and Cellular Biology, Vol. 24, No. 3, 01.02.2004, p. 1174-1187.

Research output: Contribution to journalArticle

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