Human thymus antigen: Characterization and its expression on human leukemias

Junichiro Fujimoto, Yoshifumi Ishii, Hirofumi Koshiba, Akihiro Matsuura, Minoru Ogasawara, Toshimitsu Uede, Kokichi Kikuchi

Research output: Contribution to journalArticle

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Abstract

Specific anti‐human thymus xenoantiserum (ATS) was utilized for characterizing a human thymus antigen (HTA) preferentially expressed on human thymocytes. Binding of ATS with different cell types was studied by immunofluorescence and immunoperoxidase techniques, as well as by radioimmunoprecipitation (RIP) followed by SDS‐polyacrylamide gel electrophoresis (SDS‐PAGE). ATS reacted selectively with the majority (87%) of human thymocytes among normal lymphoid cell populations tested. In the thymus, HTA was expressed largely on cortical thymocytes but not on those cells located in the thymic medulla. When 20 cases of different types of lymphatic leukemias were studied with ATS, HTA was found on acute lymphoblastic leukemia cells rosetting with sheep erythrocytes (T‐ALL) but not on other lymphatic leukemia cases, including chronic lymphocytic leukemia cells rosetting with sheep red blood cells (T‐CLL). SDS‐PAGE analysis of immunoprecipitates formed between ATS and radiolabeled cell‐surface components of human thymocytes demonstrated that ATS bound a 48,000‐molecular‐weight component that could be adsorbed to Sepharose 4B coupled with Lens culinaris hemagglutinin and could be labeled by periodate‐tritiated borohydride, indicating that HTA is a sialoglycoprotein. Similar antigen molecules were also precipitated by ATS from T‐ALL cells but not from other lymphatic leukemia cells. Five T‐ALL‐derived cell lines were tested with ATS by immunofluorescence and by RIP and SDS‐PAGE, but only two cell lines (MOLT‐3 and P12/Ich) possessed HTA molecules on their cell membrane.

Original languageEnglish
Pages (from-to)145-156
Number of pages12
JournalAmerican Journal of Hematology
Volume10
Issue number2
DOIs
Publication statusPublished - 01-01-1981

Fingerprint

Thymus Gland
Leukemia
Antigens
Thymocytes
Electrophoresis
Gels
Fluorescent Antibody Technique
Sheep
Erythrocytes
Sialoglycoproteins
Lens Plant
Cell Line
Borohydrides
Hemagglutinins
B-Cell Chronic Lymphocytic Leukemia
Immunoenzyme Techniques
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Sepharose
Cell Membrane
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Fujimoto, Junichiro ; Ishii, Yoshifumi ; Koshiba, Hirofumi ; Matsuura, Akihiro ; Ogasawara, Minoru ; Uede, Toshimitsu ; Kikuchi, Kokichi. / Human thymus antigen : Characterization and its expression on human leukemias. In: American Journal of Hematology. 1981 ; Vol. 10, No. 2. pp. 145-156.
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abstract = "Specific anti‐human thymus xenoantiserum (ATS) was utilized for characterizing a human thymus antigen (HTA) preferentially expressed on human thymocytes. Binding of ATS with different cell types was studied by immunofluorescence and immunoperoxidase techniques, as well as by radioimmunoprecipitation (RIP) followed by SDS‐polyacrylamide gel electrophoresis (SDS‐PAGE). ATS reacted selectively with the majority (87{\%}) of human thymocytes among normal lymphoid cell populations tested. In the thymus, HTA was expressed largely on cortical thymocytes but not on those cells located in the thymic medulla. When 20 cases of different types of lymphatic leukemias were studied with ATS, HTA was found on acute lymphoblastic leukemia cells rosetting with sheep erythrocytes (T‐ALL) but not on other lymphatic leukemia cases, including chronic lymphocytic leukemia cells rosetting with sheep red blood cells (T‐CLL). SDS‐PAGE analysis of immunoprecipitates formed between ATS and radiolabeled cell‐surface components of human thymocytes demonstrated that ATS bound a 48,000‐molecular‐weight component that could be adsorbed to Sepharose 4B coupled with Lens culinaris hemagglutinin and could be labeled by periodate‐tritiated borohydride, indicating that HTA is a sialoglycoprotein. Similar antigen molecules were also precipitated by ATS from T‐ALL cells but not from other lymphatic leukemia cells. Five T‐ALL‐derived cell lines were tested with ATS by immunofluorescence and by RIP and SDS‐PAGE, but only two cell lines (MOLT‐3 and P12/Ich) possessed HTA molecules on their cell membrane.",
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Fujimoto, J, Ishii, Y, Koshiba, H, Matsuura, A, Ogasawara, M, Uede, T & Kikuchi, K 1981, 'Human thymus antigen: Characterization and its expression on human leukemias', American Journal of Hematology, vol. 10, no. 2, pp. 145-156. https://doi.org/10.1002/ajh.2830100206

Human thymus antigen : Characterization and its expression on human leukemias. / Fujimoto, Junichiro; Ishii, Yoshifumi; Koshiba, Hirofumi; Matsuura, Akihiro; Ogasawara, Minoru; Uede, Toshimitsu; Kikuchi, Kokichi.

In: American Journal of Hematology, Vol. 10, No. 2, 01.01.1981, p. 145-156.

Research output: Contribution to journalArticle

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