Human ubiquitin-protein ligase Nedd4: Expression, subcellular localization and selective interaction with ubiquitin-conjugating enzymes

Tadashi Anan, Yoichi Nagata, Hisashi Koga, Yoshiomi Honda, Nami Yabuki, Chikara Miyamoto, Akira Kuwano, Ichiro Matsuda, Fumio Endo, Hideyuki Saya, Mitsuyoshi Nakao

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)


Background: Nedd4 is a ubiquitin-protein ligase containing a calcium/lipid-binding domain, multiple WW domains and a C-terminal Hect domain, which is required for both the ubiquitin transfer and the association with E2 ubiquitin-conjugating enzymes. Nedd4 has been reported to be involved in the selective ubiquitination of some regulatory proteins in transcription and membrane transport. Results: Three mRNA species for human Nedd4 were found to be 6.4-, 7.8- and 9.5-kb in size, and their expression patterns varied among normal tissues and cancer cell lines, indicating the tissue- and cell-specificities of Nedd4 expression. The Nedd4 protein, ≃120 kDa in weight, was found in the cytoplasm, mainly in the perinuclear region and cytoplasmic periphery, of human cultured cells. Neural differentiation induced not only the down-regulation of Nedd4 but also the localization of the protein to both the cytoplasm and neurites. To identify the ubiquitination pathway that is linked to Nedd4, we demonstrated that specific E2 enzymes, including human Ubc4, UbcH5B, UbcH5C, UbcH6 and UbcH7, could transfer ubiquitin molecules to Nedd4 at the active cysteine residue, whereas E6AP accepted ubiquitins from Ubc4, UbcH5B, UbcH5C and UbcH7. Furthermore, nuclear localization of N-terminal deletion mutant Nedd4 enabled us to investigate the interaction between Nedd4 and E2 enzyme (Ubc4 or UbcH7) in the cell. The simultaneous expression of the full-length Nedd4 and E2 enzyme revealed that both proteins mostly colocalized in the cytoplasmic periphery, while the N-terminal deleted Nedd4 induced the nuclear and perinuclear colocalization with E2 enzyme. Conclusion: Our findings suggested that Nedd4 plays an important role in the cell regulation, including neural differentiation through cooperation with specific E2 ubiquitination pathways.

Original languageEnglish
Pages (from-to)751-763
Number of pages13
JournalGenes to Cells
Issue number11
Publication statusPublished - 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology


Dive into the research topics of 'Human ubiquitin-protein ligase Nedd4: Expression, subcellular localization and selective interaction with ubiquitin-conjugating enzymes'. Together they form a unique fingerprint.

Cite this