TY - JOUR
T1 - Humoral immunoresponse to varicella-zoster virus pernasally coadministered with Escherichia coli enterotoxin in mice
AU - Tsuji, Takao
AU - Shiraki, Kimiyasu
AU - Sato, Hiroshi
AU - Yue-Mea, Jiang
AU - Honma, Yasuko
AU - Yoshikawa, Tetsushi
AU - Asano, Yoshizo
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan.
PY - 2000/4/3
Y1 - 2000/4/3
N2 - It is evaluated whether Escherichia coli enterotoxin is useful for induction of immunity to varicella-zoster virus (VZV) as a mucosal adjuvant in mice. When a commercially available live varicella vaccine (Oka strain) and toxin were administered simultaneously via a nasal route three times at 2 or 6 month intervals, an antibody neutralizing VZV was detected in half or all of the mice vaccinated, respectively. The antibody specific to the vaccine strain of VZV reacted to five proteins, molecular weights of which were 110 K, 100 K, 62 K, 54 K and 46 K. These proteins were composed of glycosylated products of all kinds of glycoproteins. These results suggest that although a nasal administration of the vaccine without the adjuvant has little immunogenicity in mice, the simultaneous administration of the live vaccine and the toxin over a long period induces a specific humoral immunity to VZV. Copyright (C) 2000 Elsevier Science Ltd.
AB - It is evaluated whether Escherichia coli enterotoxin is useful for induction of immunity to varicella-zoster virus (VZV) as a mucosal adjuvant in mice. When a commercially available live varicella vaccine (Oka strain) and toxin were administered simultaneously via a nasal route three times at 2 or 6 month intervals, an antibody neutralizing VZV was detected in half or all of the mice vaccinated, respectively. The antibody specific to the vaccine strain of VZV reacted to five proteins, molecular weights of which were 110 K, 100 K, 62 K, 54 K and 46 K. These proteins were composed of glycosylated products of all kinds of glycoproteins. These results suggest that although a nasal administration of the vaccine without the adjuvant has little immunogenicity in mice, the simultaneous administration of the live vaccine and the toxin over a long period induces a specific humoral immunity to VZV. Copyright (C) 2000 Elsevier Science Ltd.
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U2 - 10.1016/S0264-410X(99)00487-9
DO - 10.1016/S0264-410X(99)00487-9
M3 - Article
C2 - 10706968
AN - SCOPUS:0034004292
SN - 0264-410X
VL - 18
SP - 2049
EP - 2054
JO - Vaccine
JF - Vaccine
IS - 19
ER -
