Hybrid dynamic coating with n-dodecyl β-D-maltoside and methyl cellulose for high-performance carbohydrate analysis on poly(methyl methacrylate) chips

Fuquan Dang, Kazuaki Kakehi, Jingjun Cheng, Osamu Tabata, Masaya Kurokawa, Kazuki Nakajima, Mitsuru Ishikawa, Yoshinobu Baba

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Hybrid dynamic coating using n-dodecyl β-D-maltoside (DDM) and methyl cellulose (MC) has been developed for suppression of analyte adsorption and electroosmotic flow (EOF) in a poly(methyl methacrylate) (PMMA) channel. The adsorption of APTS-labeled sugars in a PMMA channel was obviously suppressed with DDM dynamic coating; however, EOF was reduced only by a factor of ∼25%, resulting in irreproducible separations. In contrast, both analyte adsorption and EOF in a PMMA channel were efficiently minimized with MC coating; however, concentrated MC above 0.3% was required to achieve high-performance separations, which greatly increased viscosity of the solution and caused difficulties during buffer loading and rinsing. In addition, n-dodecyltrimethylammonium chloride did not show observable effects on reducing analyte adsorption, although it has the same hydrophobic alkyl chain as DDM. These results strongly indicated that the polysaccharide moiety of surface modifiers has a specific affinity to surface charges and is crucial to achieving efficient and stable dynamic coating on the PMMA surface. Hybrid dynamic coating with 0.25% DDM and 0.03% MC was found to minimize both analyte adsorption and EOF in a PMMA channel to a negligible level, while still keeping a low viscosity of the solution. High-speed and high-throughput profiling of the N-linked glycans derived from α1-acid glycoprotein, fetuin, and ribonuclease B was demonstrated in both single-channel and 10-channel PMMA chips using DDM-MC hybrid coating. We propose that DDM-MC hybrid coating might be a general method for suppressing analyte adsorption and EOF in polymer MCE devices. The current MCE-based method might be a promising alternative for high-throughput screening of carbohydrate alterations in glycoproteins.

Original languageEnglish
Pages (from-to)1452-1458
Number of pages7
JournalAnalytical Chemistry
Volume78
Issue number5
DOIs
Publication statusPublished - 01-03-2006

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Methylcellulose
Polymethyl Methacrylate
Carbohydrates
Coatings
Adsorption
Fetuin-B
Polysaccharides
Glycoproteins
Throughput
Viscosity
Surface charge
Sugars
Buffers
Screening
Polymers
Acids

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Dang, Fuquan ; Kakehi, Kazuaki ; Cheng, Jingjun ; Tabata, Osamu ; Kurokawa, Masaya ; Nakajima, Kazuki ; Ishikawa, Mitsuru ; Baba, Yoshinobu. / Hybrid dynamic coating with n-dodecyl β-D-maltoside and methyl cellulose for high-performance carbohydrate analysis on poly(methyl methacrylate) chips. In: Analytical Chemistry. 2006 ; Vol. 78, No. 5. pp. 1452-1458.
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abstract = "Hybrid dynamic coating using n-dodecyl β-D-maltoside (DDM) and methyl cellulose (MC) has been developed for suppression of analyte adsorption and electroosmotic flow (EOF) in a poly(methyl methacrylate) (PMMA) channel. The adsorption of APTS-labeled sugars in a PMMA channel was obviously suppressed with DDM dynamic coating; however, EOF was reduced only by a factor of ∼25{\%}, resulting in irreproducible separations. In contrast, both analyte adsorption and EOF in a PMMA channel were efficiently minimized with MC coating; however, concentrated MC above 0.3{\%} was required to achieve high-performance separations, which greatly increased viscosity of the solution and caused difficulties during buffer loading and rinsing. In addition, n-dodecyltrimethylammonium chloride did not show observable effects on reducing analyte adsorption, although it has the same hydrophobic alkyl chain as DDM. These results strongly indicated that the polysaccharide moiety of surface modifiers has a specific affinity to surface charges and is crucial to achieving efficient and stable dynamic coating on the PMMA surface. Hybrid dynamic coating with 0.25{\%} DDM and 0.03{\%} MC was found to minimize both analyte adsorption and EOF in a PMMA channel to a negligible level, while still keeping a low viscosity of the solution. High-speed and high-throughput profiling of the N-linked glycans derived from α1-acid glycoprotein, fetuin, and ribonuclease B was demonstrated in both single-channel and 10-channel PMMA chips using DDM-MC hybrid coating. We propose that DDM-MC hybrid coating might be a general method for suppressing analyte adsorption and EOF in polymer MCE devices. The current MCE-based method might be a promising alternative for high-throughput screening of carbohydrate alterations in glycoproteins.",
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Hybrid dynamic coating with n-dodecyl β-D-maltoside and methyl cellulose for high-performance carbohydrate analysis on poly(methyl methacrylate) chips. / Dang, Fuquan; Kakehi, Kazuaki; Cheng, Jingjun; Tabata, Osamu; Kurokawa, Masaya; Nakajima, Kazuki; Ishikawa, Mitsuru; Baba, Yoshinobu.

In: Analytical Chemistry, Vol. 78, No. 5, 01.03.2006, p. 1452-1458.

Research output: Contribution to journalArticle

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T1 - Hybrid dynamic coating with n-dodecyl β-D-maltoside and methyl cellulose for high-performance carbohydrate analysis on poly(methyl methacrylate) chips

AU - Dang, Fuquan

AU - Kakehi, Kazuaki

AU - Cheng, Jingjun

AU - Tabata, Osamu

AU - Kurokawa, Masaya

AU - Nakajima, Kazuki

AU - Ishikawa, Mitsuru

AU - Baba, Yoshinobu

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N2 - Hybrid dynamic coating using n-dodecyl β-D-maltoside (DDM) and methyl cellulose (MC) has been developed for suppression of analyte adsorption and electroosmotic flow (EOF) in a poly(methyl methacrylate) (PMMA) channel. The adsorption of APTS-labeled sugars in a PMMA channel was obviously suppressed with DDM dynamic coating; however, EOF was reduced only by a factor of ∼25%, resulting in irreproducible separations. In contrast, both analyte adsorption and EOF in a PMMA channel were efficiently minimized with MC coating; however, concentrated MC above 0.3% was required to achieve high-performance separations, which greatly increased viscosity of the solution and caused difficulties during buffer loading and rinsing. In addition, n-dodecyltrimethylammonium chloride did not show observable effects on reducing analyte adsorption, although it has the same hydrophobic alkyl chain as DDM. These results strongly indicated that the polysaccharide moiety of surface modifiers has a specific affinity to surface charges and is crucial to achieving efficient and stable dynamic coating on the PMMA surface. Hybrid dynamic coating with 0.25% DDM and 0.03% MC was found to minimize both analyte adsorption and EOF in a PMMA channel to a negligible level, while still keeping a low viscosity of the solution. High-speed and high-throughput profiling of the N-linked glycans derived from α1-acid glycoprotein, fetuin, and ribonuclease B was demonstrated in both single-channel and 10-channel PMMA chips using DDM-MC hybrid coating. We propose that DDM-MC hybrid coating might be a general method for suppressing analyte adsorption and EOF in polymer MCE devices. The current MCE-based method might be a promising alternative for high-throughput screening of carbohydrate alterations in glycoproteins.

AB - Hybrid dynamic coating using n-dodecyl β-D-maltoside (DDM) and methyl cellulose (MC) has been developed for suppression of analyte adsorption and electroosmotic flow (EOF) in a poly(methyl methacrylate) (PMMA) channel. The adsorption of APTS-labeled sugars in a PMMA channel was obviously suppressed with DDM dynamic coating; however, EOF was reduced only by a factor of ∼25%, resulting in irreproducible separations. In contrast, both analyte adsorption and EOF in a PMMA channel were efficiently minimized with MC coating; however, concentrated MC above 0.3% was required to achieve high-performance separations, which greatly increased viscosity of the solution and caused difficulties during buffer loading and rinsing. In addition, n-dodecyltrimethylammonium chloride did not show observable effects on reducing analyte adsorption, although it has the same hydrophobic alkyl chain as DDM. These results strongly indicated that the polysaccharide moiety of surface modifiers has a specific affinity to surface charges and is crucial to achieving efficient and stable dynamic coating on the PMMA surface. Hybrid dynamic coating with 0.25% DDM and 0.03% MC was found to minimize both analyte adsorption and EOF in a PMMA channel to a negligible level, while still keeping a low viscosity of the solution. High-speed and high-throughput profiling of the N-linked glycans derived from α1-acid glycoprotein, fetuin, and ribonuclease B was demonstrated in both single-channel and 10-channel PMMA chips using DDM-MC hybrid coating. We propose that DDM-MC hybrid coating might be a general method for suppressing analyte adsorption and EOF in polymer MCE devices. The current MCE-based method might be a promising alternative for high-throughput screening of carbohydrate alterations in glycoproteins.

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