TY - JOUR
T1 - Hydroxylation of oleanolic acid to queretaroic acid by cytochrome P450 from Nonomuraea recticatena
AU - Fujii, Yoshikazu
AU - Hirosue, Shinji
AU - Fujii, Tadashi
AU - Matsumoto, Naoki
AU - Agematu, Hitosi
AU - Arisawa, Akira
PY - 2006
Y1 - 2006
N2 - A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.
AB - A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.
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U2 - 10.1271/bbb.60126
DO - 10.1271/bbb.60126
M3 - Article
C2 - 16960364
AN - SCOPUS:33749018513
SN - 0916-8451
VL - 70
SP - 2299
EP - 2302
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 9
ER -