Hydroxylation of oleanolic acid to queretaroic acid by cytochrome P450 from Nonomuraea recticatena

Yoshikazu Fujii; Shinji Hirosue; Tadashi Fujii; Naoki Matsumoto; Hitosi Agematu; Akira Arisawa

doi:10.1271/bbb.60126

Hydroxylation of oleanolic acid to queretaroic acid by cytochrome P450 from Nonomuraea recticatena

Yoshikazu Fujii
, Shinji Hirosue
, Tadashi Fujii
, Naoki Matsumoto
, Hitosi Agematu
, Akira Arisawa

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.

Original language	English
Pages (from-to)	2299-2302
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	70
Issue number	9
DOIs	https://doi.org/10.1271/bbb.60126
Publication status	Published - 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Medicine

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10.1271/bbb.60126

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abstract = "A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17\%.",

author = "Yoshikazu Fujii and Shinji Hirosue and Tadashi Fujii and Naoki Matsumoto and Hitosi Agematu and Akira Arisawa",

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T1 - Hydroxylation of oleanolic acid to queretaroic acid by cytochrome P450 from Nonomuraea recticatena

AU - Fujii, Yoshikazu

AU - Hirosue, Shinji

AU - Fujii, Tadashi

AU - Matsumoto, Naoki

AU - Agematu, Hitosi

AU - Arisawa, Akira

PY - 2006

Y1 - 2006

N2 - A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.

AB - A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.

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SN - 0916-8451

VL - 70

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 9

ER -

Hydroxylation of oleanolic acid to queretaroic acid by cytochrome P450 from Nonomuraea recticatena

Abstract

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