TY - JOUR
T1 - Hyper-phosphorylated retinoblastoma protein suppresses telomere elongation
AU - Takemura, Masaharu
AU - Sugimura, Kazuto
AU - Okumura, Katsuzumi
AU - Limsirichaikul, Siripan
AU - Suzuki, Motoshi
AU - Yamada, Yoshiji
AU - Yoshida, Shonen
N1 - Funding Information:
We thank F. Ishikawa and A. Nabetani of Kyoto University for providing antibodies to TRF1. This study was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2008
Y1 - 2008
N2 - Immortalized cell lines maintain telomeres by the expression of telomerase or by a mechanism designated alternative lengthening of telomeres (ALT). Although DNA polymerase α (pol-α) is reported to be required for telomere maintenance, the critical role of pol-α in telomere maintenance has not been firmly determined. We examined the role of retinoblastoma protein (pRb) and pol-α in the regulation of telomere length, using telomere-fiber FISH. Telomere length varied dependent on the intracellular abundance of pol-α or pRb in HeLa cells. A proportion of hyper-phosphorylated pRb (ppRb) molecules localized to sites of telomeric DNA replication in HeLa cells. Pol-α might thus contribute to telomere maintenance, and might be regulated by ppRb.
AB - Immortalized cell lines maintain telomeres by the expression of telomerase or by a mechanism designated alternative lengthening of telomeres (ALT). Although DNA polymerase α (pol-α) is reported to be required for telomere maintenance, the critical role of pol-α in telomere maintenance has not been firmly determined. We examined the role of retinoblastoma protein (pRb) and pol-α in the regulation of telomere length, using telomere-fiber FISH. Telomere length varied dependent on the intracellular abundance of pol-α or pRb in HeLa cells. A proportion of hyper-phosphorylated pRb (ppRb) molecules localized to sites of telomeric DNA replication in HeLa cells. Pol-α might thus contribute to telomere maintenance, and might be regulated by ppRb.
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U2 - 10.1271/bbb.70715
DO - 10.1271/bbb.70715
M3 - Article
C2 - 18256459
AN - SCOPUS:40449132519
SN - 0916-8451
VL - 72
SP - 630
EP - 635
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 2
ER -