Hyper-phosphorylated retinoblastoma protein suppresses telomere elongation

Masaharu Takemura, Kazuto Sugimura, Katsuzumi Okumura, Siripan Limsirichaikul, Motoshi Suzuki, Yoshiji Yamada, Shonen Yoshida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Immortalized cell lines maintain telomeres by the expression of telomerase or by a mechanism designated alternative lengthening of telomeres (ALT). Although DNA polymerase α (pol-α) is reported to be required for telomere maintenance, the critical role of pol-α in telomere maintenance has not been firmly determined. We examined the role of retinoblastoma protein (pRb) and pol-α in the regulation of telomere length, using telomere-fiber FISH. Telomere length varied dependent on the intracellular abundance of pol-α or pRb in HeLa cells. A proportion of hyper-phosphorylated pRb (ppRb) molecules localized to sites of telomeric DNA replication in HeLa cells. Pol-α might thus contribute to telomere maintenance, and might be regulated by ppRb.

Original languageEnglish
Pages (from-to)630-635
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number2
DOIs
Publication statusPublished - 13-03-2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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