Hyper-phosphorylated retinoblastoma protein suppresses telomere elongation

Masaharu Takemura, Kazuto Sugimura, Katsuzumi Okumura, Siripan Limsirichaikul, Motoshi Suzuki, Yoshiji Yamada, Shonen Yoshida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Immortalized cell lines maintain telomeres by the expression of telomerase or by a mechanism designated alternative lengthening of telomeres (ALT). Although DNA polymerase α (pol-α) is reported to be required for telomere maintenance, the critical role of pol-α in telomere maintenance has not been firmly determined. We examined the role of retinoblastoma protein (pRb) and pol-α in the regulation of telomere length, using telomere-fiber FISH. Telomere length varied dependent on the intracellular abundance of pol-α or pRb in HeLa cells. A proportion of hyper-phosphorylated pRb (ppRb) molecules localized to sites of telomeric DNA replication in HeLa cells. Pol-α might thus contribute to telomere maintenance, and might be regulated by ppRb.

Original languageEnglish
Pages (from-to)630-635
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number2
DOIs
Publication statusPublished - 13-03-2008

Fingerprint

Retinoblastoma Protein
Telomere
Elongation
Telomerase
DNA-Directed DNA Polymerase
Maintenance
HeLa Cells
Cells
Telomere Homeostasis
Molecules
Fibers
DNA
DNA Replication
Cell Line

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Takemura, M., Sugimura, K., Okumura, K., Limsirichaikul, S., Suzuki, M., Yamada, Y., & Yoshida, S. (2008). Hyper-phosphorylated retinoblastoma protein suppresses telomere elongation. Bioscience, Biotechnology and Biochemistry, 72(2), 630-635. https://doi.org/10.1271/bbb.70715
Takemura, Masaharu ; Sugimura, Kazuto ; Okumura, Katsuzumi ; Limsirichaikul, Siripan ; Suzuki, Motoshi ; Yamada, Yoshiji ; Yoshida, Shonen. / Hyper-phosphorylated retinoblastoma protein suppresses telomere elongation. In: Bioscience, Biotechnology and Biochemistry. 2008 ; Vol. 72, No. 2. pp. 630-635.
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Takemura, M, Sugimura, K, Okumura, K, Limsirichaikul, S, Suzuki, M, Yamada, Y & Yoshida, S 2008, 'Hyper-phosphorylated retinoblastoma protein suppresses telomere elongation', Bioscience, Biotechnology and Biochemistry, vol. 72, no. 2, pp. 630-635. https://doi.org/10.1271/bbb.70715

Hyper-phosphorylated retinoblastoma protein suppresses telomere elongation. / Takemura, Masaharu; Sugimura, Kazuto; Okumura, Katsuzumi; Limsirichaikul, Siripan; Suzuki, Motoshi; Yamada, Yoshiji; Yoshida, Shonen.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 2, 13.03.2008, p. 630-635.

Research output: Contribution to journalArticle

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