TY - JOUR
T1 - Hypodermal expression of Caenorhabditis elegans TGF-β type I receptor SMA-6 is essential for the growth and maintenance of body length
AU - Yoshida, Satoru
AU - Morita, Kiyokazu
AU - Mochii, Makoto
AU - Ueno, Naoto
N1 - Funding Information:
We thank A. Fire, S. Xu, J. Aynn, and G. Seydoux for providing various vectors; A. Coulson for cosmids; R. Padgett for sma-6 cDNA; the C. elegans community for numerous personal communications; the C. elegans Genome Sequencing Center for invaluable genome sequence data; and many members of the N. Ueno laboratory for suggestions. Some C. elegans strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the National Institutes of Health National Center for Research Resources (NCRR).
PY - 2001/12/1
Y1 - 2001/12/1
N2 - There are several transforming growth factor-β (TGF-β) pathways in the nematode Caenorhabditis elegans. One of these pathways regulates body length and is composed of the ligand DBL-1, serine/threonine protein kinase receptors SMA-6 and DAF-4, and cytoplasmic signaling components SMA-2, SMA-3, and SMA-4. To further examine the molecular mechanisms of body-length regulation in the nematode by the TGF-β pathway, we examined the regional requirement for the type-I receptor SMA-6. Using a SMA-6::GFP (green fluorescent protein) reporter gene, sma-6 was highly expressed in the hypodermis, unlike the type-II receptor DAF-4, which is reported to be ubiquitously expressed. We then examined the ability of SMA-6 expression in different regions of the C. elegans body to rescue the sma-6 phenotype (small) and found that hypodermal expression of SMA-6 is necessary and sufficient for the growth and maintenance of body length. We also demonstrate that GATA sequences in the sma-6 promoter contribute to the hypodermal expression of sma-6.
AB - There are several transforming growth factor-β (TGF-β) pathways in the nematode Caenorhabditis elegans. One of these pathways regulates body length and is composed of the ligand DBL-1, serine/threonine protein kinase receptors SMA-6 and DAF-4, and cytoplasmic signaling components SMA-2, SMA-3, and SMA-4. To further examine the molecular mechanisms of body-length regulation in the nematode by the TGF-β pathway, we examined the regional requirement for the type-I receptor SMA-6. Using a SMA-6::GFP (green fluorescent protein) reporter gene, sma-6 was highly expressed in the hypodermis, unlike the type-II receptor DAF-4, which is reported to be ubiquitously expressed. We then examined the ability of SMA-6 expression in different regions of the C. elegans body to rescue the sma-6 phenotype (small) and found that hypodermal expression of SMA-6 is necessary and sufficient for the growth and maintenance of body length. We also demonstrate that GATA sequences in the sma-6 promoter contribute to the hypodermal expression of sma-6.
KW - Body length
KW - Caenorhabditis elegans
KW - Hypodermis
KW - Sma/Mab pathway
KW - TGF-β
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U2 - 10.1006/dbio.2001.0443
DO - 10.1006/dbio.2001.0443
M3 - Article
C2 - 11784045
AN - SCOPUS:0035584861
SN - 0012-1606
VL - 240
SP - 32
EP - 45
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -